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Leopoldsburg (Municipality, Province of Limburg, Belgium) Senyati Safari Camp
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FAST DATING NEAR GEMBLOUX BELGIUM

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European Research and Innovation Days | Brussels, Belgium Find the best place to
stay — Belfry of Gembloux



Author information Article notes Copyright and License information Disclaimer.
Received Dec 30; Accepted Feb Associated Data Supplementary Materials
microorganismss Abstract Studies in plant-microbiome currently use diverse
protocols, making their comparison difficult and biased. Keywords: plant
microbiome, bias, harvesting protocol, standardization, apple fruit, lettuce
roots.

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 7. Establishing the Nitrogen Dilution Curve for Potato Cultivar Bintje in
    Belgium | SpringerLink?

Introduction Plant tissues provide several niches for microbial growth and a
rough distinction can be made between the aboveground plant organs, referred to
as phyllosphere and the belowground microbial niches: the rhizosphere the soil
directly surrounding plant roots from which the physicochemical properties are
influenced by the root ; the rhizoplane the root surface ; and the root
endosphere the compartment formed by the apoplastic spaces between the root
cells.

Table 1 Diversity of protocol for harvesting microbiomes from apple carposphere
and lettuce roots. Open in a separate window. Materials and Methods 2. Table 2
Methods description of apple carposphere harvesting A and lettuce rhizoplane
harvesting B. Results 3. Apple Carposphere Microbiota 3. Figure 1. Figure 2.
Figure 3. Lettuce Rhizoplane Microbiota 3. Cultivable Microbiota Enumeration The
results of the average cumulative number of CFU harvested by each protocol
following successive washes are illustrated in Figure 4.

Figure 4.


EUROPEAN RESEARCH AND INNOVATION DAYS | BRUSSELS, BELGIUM

Figure 5. Figure 6. Discussion In the scientific history, any emerging concept
relying on fast moving technologies has been prone to very important bias and
errors at its infancy. Impact of the Protocol on the Quantity and Diversity of
Harvested Microorganisms Between studies found in the literature, protocols
differ in washing buffers water, phosphate, saline, or phosphate saline ,
washing time, washing methodology [washing, grinding therefore also including
endophytes , shaking, sonication, or a combination of them].

Conclusions These two study cases indicate that the washing protocol
significantly influences the quantity and the bacterial diversity of
microorganisms harvested and that four successive washes can increase up to
three-times the quantity of harvested microorganisms. Click here for additional
data file. Author Contributions Conceptualization, A. Conflicts of Interest The
authors declare no conflict of interest. References 1. Reinhold-Hurek B.
Lugtenberg B. Plant-growth-promoting rhizobacteria. Compant S. Plant
growth-promoting bacteria in the rhizo- and endosphere of plants: Their role,
colonization, mechanisms involved and prospects for utilization.

Soil Biol. Vorholt J. Microbial life in the phyllosphere. Bringel F. Pivotal
roles of phyllosphere microorganisms at the interface between plant functioning
and atmospheric trace gas dynamics. Glassner H. Characterization of endophytic
bacteria from cucurbit fruits with potential benefits to agriculture in melons
Cucumis melo L.

FEMS Microbiol. Ramey B. Von, Fuqua C. Biofilm formation in plant-microbe
associations. Abdelfattah A. Spatial and compositional variation in the fungal
communities of organic and conventionally grown apple fruit at the consumer
point-of-purchase. Massart S. Biological control in the microbiome era:
Challenges and opportunities.

Izard J. Metagenomics for Microbiology.

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Elsevier Inc. Pollock J. Boers S. Understanding and overcoming the pitfalls and
biases of next-generation sequencing NGS methods for use in the routine clinical
microbiological diagnostic laboratory. Barillot C. A standardized method for the
sampling of rhizosphere and rhizoplan soil bacteria associated to a herbaceous
root system. Knief C. Analysis of plant microbe interactions in the era of next
generation sequencing technologies.

Plant Sci. Tian X. Song Z. Run-to-run sequencing variation can introduce
taxon-specific bias in the evaluation of fungal microbiomes.

Phytobiomes J. Kloepper J. A review of issues related to measuring colonization
of plant roots by bacteria. Donegan K. Evaluation of methods for sampling,
recovery, and enumeration of bacteria applied to the phylloplane.
Richter-heitmann T. Evaluation of strategies to separate root-associated
microbial communities: A crucial choice in rhizobiome research.

Yeasts and yeast-like organisms associated with fruits and blossoms of different
fruit trees. Wilson C. A selection strategy for microbial antagonists to control
postharvest diseases of fruits and vegetables. Acknowledgments Restek Corp.
References 1. Vass AA Beyond the grave-understanding human decomposition.
Microbiol Today — View Article Google Scholar 2. J Forensic Sci — View Article
Google Scholar 3. Statheropoulos M, Spiliopoulou C, Agapiou A A study of
volatile organic compounds evolved from decaying human body.

Forensic Sci Int — View Article Google Scholar 4. Appl Micribiol Biotechnol —
View Article Google Scholar 5. Environ Geol — View Article Google Scholar 6.
Boumba VA, Ziavrou KS, Vougiouklakis T Biochemical pathways generating
post-mortem volatile compounds co —detected during forensic ethanol analyses.
View Article Google Scholar 7. View Article Google Scholar 8.

View Article Google Scholar 9. J Forensic Sci 3: — View Article Google Scholar
Statheropoulos M, Mikedi K, Agapiou A, Georgiadou A, Karma S Discrimant analysis
of volatile organic compounds data related to a new location method of entrapped
people in collapsed buildings of an earthquake. Analytica Chimica Acta — Sci Tot
Envir — Forensic Sci Int 6— Anal Bioanal Chem — Forensic Sci Int 46— Bioresour
Technol 71— Naturwissenschaften — Int J Legal Med 43— Forensic Sci Int 35—
Springfield: Thomas.

Komar D The use of cadaver dogs in locating scattered, scavenged human remains:
preliminary fields test results. Environ Monit Assess — New York: Springer.
Biotechnol Agron Soc Environ — Eur J Lipid Sci Technol 73— Analytica Chimica
Acta 9— London: Academic Press Ltd. Anal Chem A—A. J High Resolut Chromatogr —
Talanta — Phillips JB, Beens J Comprehensive two-dimensional gas chromatography:
a hyphenated method with strong coupling between the two dimensions. J
Chromatogr A — J Chromatogr A 45— In: Ramos L, editor.

Amsterdam: Elsevier. Silva I, Rocha SM, Coimbra MA, Marriott PJ Headspace
solid-phase microextraction combined with comprehensive two-dimensional gas
chromatography time-of-flight mass spectrometry for the determination of
volatile compounds from marine salt. Grassberger M, Frank C Initial study of
arthropod succession on pig carrion a central European urban habitat.

J Med Entomol — Annu Rev Entomol — Antenna — J Med Entomol 44 5 : — Forensic Sci
Int 50— Harsanyi L Differential diagnosis of human and animal bone. Three main
site types where black alder grows can be identified Dethioux, ; Claessens, in
relation to the type of water supply:. Marshy sites that have waterlogged
subsoil throughout the year. On these, black alder forms pure stands owing to
its physiological adaptations that allow it to grow in anaerobic soils.

This is the Alnetum community according to the habitat classification of
Noirfalise Riverside sites in which the soil in the rooting zone is well aerated
during the growing season. On these, black alder is often mixed with ash, elm,
maple, willow and other species that are adapted to alluvial sites Alno-Padion
community. Plateau sites with high soil moisture contents, for example deep
loamy soils, generally with a temporary water-table and where the stand is
dominated by other species e. Several authors have constructed height growth
curves Sopp, ; Lockow, ; Mitscherlich, , in Schober, ; Johansson, ; Giurgiu and
Draghiciu, ; Thibaut et al.

Despite the different methods and approaches to construction, the shape of
curves is always very similar Figure 3. A comparison of the curves that go
through 24 m height at age 50 shows only small differences in shape despite the
fact that site index ranges differ between countries Figure 4. It seems from
them that alder grows faster in southeastern Europe Hungary and Romania when
young Figure 3. Periodic measurements of mean or top height and age on permanent
sample plots.

Top height is the mean height of the largest diameter trees per hectare in the
stand. Range of site index height at 50 years of black alder for different
countries in Europe complete references in Table 1. All models show that black
alder height growth pattern is characterized by a very high growth rates when
young Martin, , Lockow, , and Thibaut et al. By age 25, black alder reaches half
the height it will achieve at maturity. Considering all the species together on
a suitable site, the height growth of black alder is similar to that of ash and
birch up to age Height or diameter reached at a reference age i.


FIND THE BEST PLACE TO STAY — BELFRY OF GEMBLOUX

Published site index curves range from In the sets of site index curves, the
differences are evident by age 20 5. On the best sites in Northern Germany,
Slovenia and Southern France, records exist of black alder having a height of 35
m at maturity Glavac, ; Lhote, The rectangle highlights different diameters in a
same age class in relation with different competition levels Claessens et al. In
Belgium, when modelling height growth, Thibaut et al.

The genetic origin can also have a very marked influence on the pattern of
growth. In The Netherlands, Verweij has shown the existence of a lowland peat
ecotype that grows fast for the first 10—15 years but then slows down very
considerably. This ecotype has been grown widely in shelterbelts but is not
suitable for timber production. Its use was thought by Schmidt-Vogt , Liepe ,
Franke , Cech and Hendry to explain the alder decline observed during the early
part of the twentieth century in German plantations. Glavac , Stortelder et al.

All authors have found that productivity is greatest on riverside sites followed
by plateau sites with high levels of soil moisture. These are followed by marshy
sites and plateau sites that suffer from periods of soil drought. Stortelder et
al. Despite the fact that alder is the only tree that will grow in marshes,
production is often slow and the wood of low quality in these conditions. For
alder stands in Southern Belgium, Claessens developed two models for forecasting
site index.

One uses abiotic parameters, including elevation, type of humus and ecoregion,
and the other uses vegetation types.

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