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Skip to main content Global Developer, Manufacturer, and Supplier of High-Quality Reagents, Analytical Instruments, and Precision Diagnostics. INCLUDES R&D SystemsTM Novus BiologicalsTM Tocris BioscienceTM ProteinSimpleTM ACDTM ExosomeDxTM Asuragen® LunaphoreTM +44 1235 529449 United Kingdom - (English) Country/Region selector Please enter your country/region. 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About Bio-Techne Bio-Techne Brands Corporate & Social Responsibility Investor Relations Quality Latest at Bio-Techne -------------------------------------------------------------------------------- Careers Events Programs & Promotions Support -------------------------------------------------------------------------------- Contact & Support Distributors BREADCRUMB 1. Home 2. Products 3. Multiplex Cytokine Assay Kits 4. Multiplex Cytokine Assay Kits Luminex Assays 5. Human Tumor Biomarker Luminex® Performance Assay (FCSTM25) HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY R&D SYSTEMS, PART OF BIO-TECHNE | CATALOG # FCSTM25 (1) Product Datasheet / COA / SDS (11) LUMINEX HIGH PERFORMANCE ASSAYS Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure you are getting the best performance out of your multiplex assay. Panels are customizable so you can order a selection of the analytes or the entire panel. Build your own Luminex Assay with our Luminex Assay Customization Tool. -------------------------------------------------------------------------------- Build Now KEY PRODUCT DETAILS Panel: Luminex Performance Human Tumor Biomarker Panel Species: Human Analytes Detected: * CA125/MUC16 * CA15-3/MUC-1 * CA19-9 * CD40 Ligand/TNFSF5 * CEACAM-5/CD66e * CYFRA21-1 * Chorionic Gonadotropin beta Chain (HCG beta) * Chromogranin A * Enolase 2/Neuron-specific Enolase * FGF basic/FGF2/bFGF * Fas/TNFRSF6/CD95 * Fas Ligand/TNFSF6 * Glypican 1 * HE4/WFDC2 * HGF * IL-6 * IL-8/CXCL8 * Kallikrein 3/PSA * Leptin/OB * MIF * Osteopontin/OPN * Prolactin * SCF/c-kit Ligand * TGF-alpha * TNF-alpha * Thyroglobulin * VEGF * alpha-Fetoprotein/AFP Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services . ORDER INFORMATION Bio-Techne Ltd. tel: +44 1235 529449 tel: 0800373415 (free phone) Contact Us » 100% Guarantee on all R&D Systems products. SHIPPING INFORMATION Free Shipping to United Kingdom Products in stock will be shipped in 1-3 business days unless specified. Setting the standard in quality research reagents KEY PRODUCT DETAILS Panel: Luminex Performance Human Tumor Biomarker Panel Species: Human Analytes Detected: * CA125/MUC16 * CA15-3/MUC-1 * CA19-9 * CD40 Ligand/TNFSF5 * CEACAM-5/CD66e * CYFRA21-1 * Chorionic Gonadotropin beta Chain (HCG beta) * Chromogranin A * Enolase 2/Neuron-specific Enolase * FGF basic/FGF2/bFGF * Fas/TNFRSF6/CD95 * Fas Ligand/TNFSF6 * Glypican 1 * HE4/WFDC2 * HGF * IL-6 * IL-8/CXCL8 * Kallikrein 3/PSA * Leptin/OB * MIF * Osteopontin/OPN * Prolactin * SCF/c-kit Ligand * TGF-alpha * TNF-alpha * Thyroglobulin * VEGF * alpha-Fetoprotein/AFP Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services . VIEW ALL MULTIPLEX CYTOKINE ASSAY KITS LUMINEX ASSAYS » View Full Technical Details for Human Tumor Biomarker Luminex® Performance Assay * Technical Data * Citations / Reviews (1) Product Features Performance Validation Kit Components Precision and Accuracy Build Your Assay Which Luminex Assay is right for me? About Magnetic Luminex Assays Scientific Data Preparation and Storage Product Specific Notices Product Datasheets PRODUCT FEATURES FOR HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples. * Simultaneously profile up to 28 analytes of your choice in one sample * Magnetic format allows for easier wash steps * Undergoes similar validation testing as Quantikine® ELISA Kits * Precise and Specific * Requires a small sample volume (<50 µL) * Run your assay in just 3-5 hours * Mass-calibrated standards for consistent results with every new lot of material * Enough reagents for one 96-well plate PERFORMANCE VALIDATION FOR HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet. KIT COMPONENTS FOR HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY * Premixed cocktail of antibody-coated Magnetic beads * Premixed cocktail of biotinylated detection antibody * Standard Cocktail(s) * Bead Diluent * Biotin Antibody Diluent * Calibrator Diluent * Wash Buffer * Streptavidin-PE * One flat-bottom 96-well Microplate * Foil Plate Sealers (4) * Mixing Bottle * Standard Value Card** **A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay. PRECISION Intra-Assay Precision - is generated from the mean of the %CV’s from 40 reportable results across two different concentrations of analytes in a single serum assay. Inter-Assay Precision - is generated from the mean of the %CV’s across two different concentrations of analytes across thirty six different serum assays. n=40 (20x2 levels) n=36 Analyte Intra-Assay (%CV) Inter-Assay (%CV) AFP 7.0 10.3 CA125/MUC16 6.8 9.9 CA15-5/MUC-1 6.3 8.4 CA19-9 4.0 10.7 CD40 Ligand/TNFSF5 4.8 8.8 CEACAM-5/CD66e 8.0 9.7 Chromogranin A 3.5 9.0 CYFRA21-1 5.7 9.7 Enolase 2 8.6 10.8 Fas/TNFRSF6/CD95 5.6 8.6 Fas Ligand 6.2 9.4 FGF basic 2.4 8.1 Glypican-1 7.9 9.7 HCG-beta 4.8 7.9 HE4/WFDC2 5.2 8.6 HGF 6.2 9.0 IL-6 3.7 8.0 IL-8/CXCL8 5.9 9.3 Leptin/OB 4.4 8.8 MIF 5.1 8.0 Osteopontin/OPN 5.9 9.2 Kallikrein 3/PSA 5.1 8.7 Prolactin 7.9 9.7 SCF/c-kit Ligand 6.1 9.7 TGF alpha 5.4 9.1 Thyroglobulin 9.0 10.0 TNF alpha 4.0 9.5 VEGF 4.9 8.7 ACCURACY Linearity - The data represents mean spiked or natural linearity in serum matrix samples (n=4). Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples (n=4). Linearity Spiked Recovery Analyte Average % Average % AFP* 117 HE CA125/MUC16 120 86.8 CA15-3/MUC-1* 113 HE CA19-9 132 63.9 CD40 Ligand/TNFSF5* 107 HE CEACAM-5/CD66e* 123 HE Chromogranin A* 111 HE CYFRA21-1 129 78.2 Enolase 2 127 62.5 Fas/TNFRSF6/CD95* 106 HE Fas Ligand 122 82.4 FGF basic 135 74.6 Glypican-1 126 72.9 HCG-B 131 58.1 HE4/WFDC2* 114 HE HGF 134 62.5 IL-6 128 65.6 IL-8/CXCL8* 111 HE Leptin/OB* 110 HE MIF* 134 HE Osteopontin/OPN* 105 HE Kallikrein-3/PSA 122 78.9 Prolactin* 106 HE SCF/c-kit Ligand 185 42.8 TGF alpha 122 77.6 Thyroglobulin 123 65.8 TNF alpha 170 54.2 VEGF 123 67.5 *Natural linearity HE - Spiked recovery was not observed due to natural linearity in high endogenous samples BUILD YOUR ASSAY MAKE YOUR SELECTIONS BELOW TO CONFIGURE YOUR CUSTOM LUMINEX ASSAY Filter Analytes Select All Select None alpha-Fetoprotein/AFP Fas/TNFRSF6/CD95 Leptin/OB CA125/MUC16 Fas Ligand/TNFSF6 MIF CA15-3/MUC-1 FGF basic/FGF2/bFGF Osteopontin/OPN CA19-9 Glypican 1 Prolactin CD40 Ligand/TNFSF5 HE4/WFDC2 SCF/c-kit Ligand CEACAM-5/CD66e HGF TGF-alpha Chorionic Gonadotropin beta Chain (HCG beta) IL-6 Thyroglobulin Chromogranin A IL-8/CXCL8 TNF-alpha CYFRA21-1 Kallikrein 3/PSA VEGF Enolase 2/Neuron-specific Enolase ASSAYS Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed. If you run into bead region incompatibilities, send your Luminex code to our team and find out more. This product is only available in the mixing displayed below. For more options, please use the full tool. Premixed Please select at least one mixing option to see the kits. WOULD YOU LIKE TO SPLIT BASED ON DILUTION? Do not split Split based on serum Split based on plasma Split based on cell culture supernates WHICH LUMINEX ASSAY IS RIGHT FOR ME? Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com . MORE INFORMATION ABOUT MAGNETIC BEAD-BASED MULTIPLEX KITS FOR THE LUMINEX® PLATFORM Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms. Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems. SCIENTIFIC DATA EXAMPLES FOR HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY Human Tumor Biomarker Luminex Panel Standard Curves Human Tumor Biomarker Luminex Panel Serum Sample Values Serum samples from 20 apparently healthy individuals were run on the Human Tumor Biomarker Luminex Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median. Human Tumor Biomarker Luminex Panel EDTA Plasma Sample Values Human Tumor Biomarker Luminex Panel Heparin Plasma Sample Values Human Tumor Biomarker Luminex Panel Cell Culture Supernate Sample Values Human Tumor Biomarker Luminex Panel Cell Lysate Sample Values Human Tumor Biomarker Luminex Panel Tissue Lysate Sample Values Human Tumor Biomarker Luminex Panel Healthy Controls vs. Colorectal Cancer Serum Samples Colorectal cancer serum samples were tested alongside healthy controls on the Human Tumor Biomarker Panel and significant differences were observed over many analytes. A selection of these are shown below. Human Tumor Biomarker Luminex Panel Healthy Controls vs. Pancreatic Cancer Serum Samples Pancreatic cancer serum samples were tested alongside healthy controls on the Human Tumor Biomarker Panel and significant differences were observed over many analytes. A selection of these are shown below. Human Tumor Biomarker Luminex Panel Protein-Plus Streck Plasma Sample Values Human Tumor Biomarker Luminex Panel Streck Plasma Sample Values PREPARATION AND STORAGE Stability & Storage Store the unopened product at 2 - 8 °C. Do not use past expiration date. PRODUCT SPECIFIC NOTICES FOR HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY Luminex® is a registered trademark of Luminex Corporation. Citations Reviews CITATIONS FOR HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY Powered by Bioz See more details on Bioz CUSTOMER REVIEWS FOR HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY 5 out of 5 1 Customer Rating 5 Stars 100% 4 Stars 0% 3 Stars 0% 2 Stars 0% 1 Stars 0% Have you used Human Tumor Biomarker Luminex® Performance Assay? Submit a review and receive an Amazon gift card! $25/€18/£15/$25CAN/¥2500 Yen for a review with an image $10/€7/£6/$10CAN/¥1110 Yen for a review without an image Submit a review CUSTOMER IMAGES * * (1) -------------------------------------------------------------------------------- Showing 1 - 1 of 1 review Showing All Filter By: All Ratings 5 Clear Filters X * HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY Name: Anonymous Sample Tested: EDTA Plasma Verified Customer | Posted 10/11/2024 There are no reviews that match your criteria. Showing 1 - 1 of 1 review Showing All * « * < * * > * » PRODUCT DOCUMENTS FOR HUMAN TUMOR BIOMARKER LUMINEX® PERFORMANCE ASSAY PRODUCT DATASHEETS Download Product Datasheets COA CERTIFICATE OF ANALYSIS To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components. SDS Download SDS Global Developer, Manufacturer, and Supplier of High-Quality Reagents, Analytical Instruments, and Precision Diagnostics. INCLUDES R&D Systems™ Novus Biologicals™ Tocris Bioscience™ ProteinSimple™ ACD™ ExosomeDx™ Asuragen™ Lunaphore™ CONTACT INFORMATION 614 McKinley Place NE Minneapolis, MN 55413 USA TEL 612 379 2956 Toll-free 800 343 7475 FAX 612 656 4400 WHO WE ARE * About Bio-Techne * Bio-Techne Brands * Careers * Corporate & Social Responsibility * Terms and Conditions INVESTOR RELATIONS * Overview * News / Events * Company Information * Financial Information * Stock Data * SEC Filings * Corporate Governance STAY CONNECTED Contact Us -------------------------------------------------------------------------------- Privacy and Cookie Policy Site Map © Copyright 2024 Bio-Techne. All Rights Reserved. Bio-Techne uses cookies to provide you with a great website experience. By continuing to use this website you acknowledge this and agree to our cookie policy. Learn more. 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