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ANTS CAN 'SNIFF OUT' CANCER TUMORS IN URINE, SAYS NEW STUDY

The researchers taught the ants to link the scent of tumors with a reward by
dabbing a drop of sugar into the water.
Nergis Firtina
| Jan 25, 2023 04:43 AM EST
Created: Jan 25, 2023 04:43 AM EST
health
 * 
 * 
 * 
 * 

Formica fusca.

FrankRamspott/iStock 

A newly published study has announced that ants can detect cancer tumors by
smell, thanks to their strong olfactory receptors. Published in the journal
Proceedings of the Royal Society B: Biological Sciences this week, the study
shows Formica fusca ants are able to detect tumors by smelling patients' urine,
as reported by The Independent.




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"Ants can be used as bio-detectors to discriminate healthy individuals from
tumor-bearing ones," says Patrizia d'Ettorre, co-author of the study and a
scholar at the Sorbonne Sorbonne Paris North University. "They are easy to
train, learn fast, are very efficient, and are not expensive to keep," she
added.

On the other hand, Scientific American underlines volatile organic compounds,
which are released specifically by cancerous tumors, are frequently found in
physiological fluids, including perspiration and urine, as well as in breath
vapor.


HOW WAS THE PROCESS?

The study's lead author, Baptiste Piqueret, an ethologist at Sorbonne Paris
North University, and his team began by xenografting human breast cancer tumors
into mice and allowing them to develop. Then they took urine samples from
healthy and tumor-ridden animals.

The researchers taught the ants to link the scent of tumors with a reward by
dabbing a drop of sugar water in front of the urine from cancer-stricken mice.
The flies lingered near the urine of malignant mice for roughly 20 percent
longer than that of healthy mice when the team withdrew the sugar water because
they were seeking a tasty treat.

Silky ant (Formica fusca).

Carine Carnier/iStock 

The ants' smell association was established in just three training sessions or
around 10 minutes overall. That's a lot quicker than, say, training canines to
detect cancer, which can take up to six months.





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In addition to Piqueret's words, "We trained them with associative learning to
associate a given odor – cancer – with a reward and, after very few trials, they
learned the association," said d'Ettore.

"We demonstrated that ants can discriminate the urine of healthy mice from the
urine of tumor-bearing mice. This is more similar to a real-life situation than
using cultured cancer cells. We were surprised by how efficient and reliable the
ants are," she added, as per The Independent.


MORE ABOUT FORMICA FUSCA

A black ant known as Formica fusca is widespread throughout Europe, as well as
in some regions of Southern Asia and Africa. Its popular names include silky ant
and dusky ant. The Palaearctic region's range stretches from Italy in the south
to Fennoscandia in the north and from Portugal in the west to Japan in the east.

Using a classical conditioning strategy, workers of this ant species can learn
to link an olfactory signal to a reward (a sugar solution). Ants are quick
learners; all it takes for them to develop a true long-term memory is one
presentation of the stimuli. Additionally, this created memory is
extinction-resistant.

The study is published in the journal Proceedings of the Royal Society B:
Biological Sciences.





Study abstract:

Early detection of cancer is critical in medical sciences, as the sooner a
cancer is diagnosed, the higher are the chances of recovery. Tumour cells are
characterized by specific volatile organic compounds (VOCs) that can be used as
cancer biomarkers. Through olfactory associative learning, animals can be
trained to detect these VOCs. Insects such as ants have a refined sense of
smell, and can be easily and rapidly trained with olfactory conditioning. Using
urine from patient-derived xenograft mice as stimulus, we demonstrate that
individual ants can learn to discriminate the odour of healthy mice from that of
tumour-bearing mice and do so after only three conditioning trials. After
training, they spend approximately 20% more time in the vicinity of the learned
odour than beside the other stimulus. Chemical analyses confirmed that the
presence of the tumour changed the urine odour, supporting the behavioural
results. Our study demonstrates that ants reliably detect tumour cues in mice
urine and have the potential to act as efficient and inexpensive cancer
bio-detectors.




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