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NEON NXT ELECTROPORATION SYSTEM:
TRUSTED TRANSFECTION PERFORMANCE


Request Demo/Quote   Protocol library


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‹Cell Transfection: High Efficiency Solutions
 * Transfection Reagents ›
 * Neon NxT Electroporation System ›
 * Transfection Applications ›
 * Overview of Transfection Methods ›
 * Transfection Resources ›



Protect your valuable research time and samples with a compact electroporation
machine designed and engineered for performance, speed, simplicity, and
flexibility. The combination of the Invitrogen Neon NxT Electroporation System’s
proprietary pipette tip technology, simplified workflow, and flexibility helps
reduce sample loss, lowers contamination risk, saves you steps, and can put you
in control of your experiments.


BENEFITS OF THE NEON NXT ELECTROPORATION SYSTEM

 * Proven transfection efficiency and cell viability—transfect challenging cell
   lines with high efficiency and high cell viability
 * Flexibility when you need it—precisely optimize electroporation parameters
   for different cell types, cell densities, payloads, and electroporation
   protocols.
 * Enhanced genome editing efficiency—optimized buffer for improved delivery of
   CRISPR molecules
 * Save valuable research time—transfect cells quickly with an easy three step
   workflow, a single buffer kit and intuitive user interface
 * Preserve your precious samples—minimize sample transfer loss and
   contamination risk

Download brochure

 

Invitrogen TransfectionLab, a cloud-based app can automatically generate
step-by-step guide tailored for each electroporation experiment, starting from
the design stage. You can remotely set up multiple plate layouts for up to 384
samples, and any protocol or plate layout saved in the app can be accessed
directly on the connected Neon NxT system.





ORDER THE NEON NXT ELECTROPORATION SYSTEM

neon1s,neon1sk,n1025,n10025,n1096,n10096,nt96,neon1p,neon1ps

Catalog # Name Size Price (CAD) Qty NEON1S Neon™ NxT Electroporation System Each
13,770.00
NEON1SK Neon™ NxT Electroporation System Starter Pack Each
18,180.00
N1025 Neon™ NxT Electroporation System 10-μL Kit 25 x 2 reactions
1,006.00
N10025 Neon™ NxT Electroporation System 100-μL Kit 25 x 2 reactions
1,012.00
N1096 Neon™ NxT Electroporation System 10-μL Kit 96 x 2 reactions
2,970.00
N10096 Neon™ NxT Electroporation System 100-μL Kit 96 x 2 reactions
2,975.00
NT96 Neon™ NxT Electroporation System Tubes 96 tubes
856.00
NEON1P Neon™ NxT Electroporation System Pipette 1 pipette
1,146.00
NEON1PS Neon™ NxT Electroporation System Pipette Station 1 station
1,660.00

NEON1S,NEON1SK,N1025,N10025,N1096,N10096,NT96,NEON1P,NEON1PS


HOW TO USE THE NEON NXT ELECTROPORATION SYSTEM




CUSTOMER STORY

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Neon NxT University Lab Partners Testimonial Video

In this video, Samantha Renusch, the Education Program Manager at University Lab
Partners, shares her experience using the Neon NxT Electroporation System.






ACHIEVE HIGH TRANSFECTION EFFICIENCY AND HIGH CELL VIABILITY IN A BROAD RANGE OF
CELL LINES USING THIS TRANSFECTION SYSTEM

The Neon NxT Electroporation System is an electroporation instrument that offers
up to 90% transfection and gene-editing efficiency in extremely
difficult-to-transfect cells, including immune, primary, and stem cells. Over
150 cell lines have been tested with optimized ready-to-use conditions for
efficiency and viability.

Search the Transfection protocols and citations database for optimized
electroporation protocols on different cell lines.

Figure 1. Transfection efficiency with the Neon NxT Electroporation System.
Cells were transfected with GFP plasmid DNA or GFP mRNA in 10 μL or 100 μL
electroporation reactions. Transfection efficiency is reported as the percentage
of GFP-positive cells (n = 3). Note: Naive T cells were only electroporated in
100 μL reactions.



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Figure 2. Cell viability following electroporation on the Neon NxT system. Cells
were transfected with GFP plasmid DNA or GFP mRNA in 10 μL or 100 μL
electroporation reactions. Transfected cells were stained with Invitrogen SYTO
Red Dead Cell Stain and assessed for viability on the Invitrogen ATTUNE NxT Flow
Cytometer. Cell viability (%) is reported as the mean of 3
measurements. Note: Naive T cells were only electroporated in 100 μL reactions.



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Figure 3. Performance of Neon NxT Resuspension Genome Editing Buffer in
CRISPR-Cas9–based genome editing experiments. Cells were electroporated in 10 μL
or 100 μL reactions. (A) GFP donor DNA knock-in efficiency reported as the
percentage of GFP-positive cells. (B) Viability of cells after GFP donor DNA
knock-in. (C) Knockout efficiency reported as percentage of initial GFP-positive
cells not expressing GFP after electroporation. (D) Post-electroporation
viability of knockout cells.




ENHANCING GENOME EDITING EFFICIENCY

Genome editing holds great promise in the pursuit of a greater understanding of
human health and disease. A critical, yet challenging step in the genome editing
workflow is the effective delivery of CRISPR ribonucleoprotein (RNP), DNA and
RNA molecules into the cell line of choice—also known as transfection. As
demonstrated above, electroporation is the most widely used delivery method for
its ability to achieve high transfection efficiency, even with hard-to-transfect
cells.

When used with the Neon NxT Electroporation System, our gene editing reagents
and the Neon NxT Resuspension Genome Editing Buffer can help improve gene
editing performance with specific payloads like CRISPR Cas9 for knockout or
knock-in experiments with mammalian cells including primary, stem, and
difficult-to-transfect cells (Figure 3).

Explore our comprehensive suite of gene editing workflow solutions at
thermofisher.com/geneediting



WHY IS THE NEON NXT ELECTROPORATION SYSTEM A BETTER ALTERNATIVE TO THE
ELECTROPORATION CUVETTE?

Figure 4. Neon NxT pipette tip advantages compared to standard electroporation
cuvette. The proprietary biologically compatible (A) Neon NxT tip presents a
proven capillary electroporation technology that has important advantages over
(B) electroporation cuvettes.

Unlike standard cuvette-based electroporation chambers, the Neon NxT
electroporation system uses a proprietary biologically compatible pipette tip
chamber that generates a more uniform electric field. This design allows for a
better maintenance of physiological conditions resulting in high cell survival
compared to conventional electroporation.†

 * Creates a more uniform electric field
 * Maintains a stable pH throughout the electroporation chamber
 * Generates minimal heat
 * Forms fewer ions
 * Reduces shear force experienced by cells






PRECISELY OPTIMIZE ELECTROPORATION PARAMETERS FOR DIFFERENT CELL TYPES, CELL
DENSITIES, PAYLOADS, AND ELECTROPORATION PROTOCOLS


CUSTOMIZABLE ELECTROPORATION PARAMETERS

With the Neon NxT Electroporation System, you can precisely control the
parameters that matter most for your experiment without wasting precious time
adjusting those that do not. This transfection system allows you to modify:

 * Pulse voltage
 * Pulse width
 * Number of pulses
 * Cell type
 * Buffer type
 * Payload type


VERSATILITY FOR DIFFERENT PAYLOADS, CELL TYPES, AND CELL DENSITIES

Deliver DNA, RNA, or protein to a wide variety of mammalian cells, with the
flexibility to transfect 1 x 104 to 1 x 107 cells per reaction.



TRANSFECT CELLS QUICKLY WITH AN EASY THREE-STEP WORKFLOW, A SINGLE BUFFER KIT,
AND AN INTUITIVE USER INTERFACE


SIMPLIFIED ELECTROPORATION WORKFLOW

Easy to follow and execute, the Neon NxT Electroporation System streamlined
workflow requires minimal training and improves consistency and reproducibility.

Using this transfection system, the electroporation process is simple: first,
take up cells and plasmid mix into the Neon NxT pipette tip. Second, plug into
the pipette holder, and press Electroporate. Finally, pipette the transfected
cells back into your culture vessel. As the transfection occurs in the pipette
tip, the transfection is completed in just 3 simple steps: Aspirate,
Electroporate, Dispense.

Step 1

ASPIRATE

Prepare cells by suspending them in a Neon NxT buffer. Load cells and payload
mix suspended in
Neon NxT buffer into the Neon NxT pipette tip.Step 2

ELECTROPORATE


Apply electrical pulses to cells to deliver the payload in the specialized
buffer.Step 3

DISPENSE

Restore growth conditions to allow the transfected cells to recover.Step 4

ANALYZE CELLS


Evaluate: e.g., gene expression, genome editing, silencing, and cell line
development


SHORTER PROCESSING TIME USING THIS ELECTROPORATION SYSTEM

In addition to simplifying the workflow, our proprietary electroporation
technology shortens the overall process to 10–15 minutes, compared to a
conventional electroporation system as the transfection occurs in the Neon NxT
pipette tip.†


A SINGLE BUFFER KIT FOR MANY MAMMALIAN CELL TYPES

Avoid the hassle of searching for a buffer kit that will work with your cell
line. We have simplified the process with one buffer kit that is compatible with
over 150 mammalian cell lines. We recommend using Invitrogen Neon NxT
Resuspension R Buffer at voltages below 1,900 V and Invitrogen Neon NxT
Resuspension T Buffer at higher voltages.


TIME-SAVING PLATE SETUP

Rather than adjusting electroporation parameters between samples with Quick
Start, set up an entire plate beforehand using the Neon NxT Electroporation
System’s intuitive user interface. Monitor progress on the screen as you
electroporate each of your samples, and electroporate up to 24 samples in less
than 20 minutes.







MINIMIZE SAMPLE TRANSFER LOSS AND CONTAMINATION RISK OF VALUABLE CELLS WITH
UNIQUE AND COMPACT ELECTROPORATION INSTRUMENT DESIGN


MINIMAL SAMPLE TRANSFER LOSS

Your samples are precious, but some volume is inevitably lost during transfer to
and from a conventional electroporation cuvette. Electroporation on the Neon NxT
Electroporation System occurs within the pipette tip, helping to minimize sample
loss and the amount of shear force experienced by cells.



MINIMAL SAMPLE CONTAMINATION

The compact footprint of the Neon NxT system, including the pulse generator and
pipette station, makes it small enough to fit in most biosafety cabinets (BSCs).
Your samples always stay within the aseptic area while using the instrument,
helping reduce the risk of contaminating precious cells. The Neon NxT system
also has a new cable management feature that makes using it in the BSC
hassle-free.

Figure 5. Dimensions of the Neon NxT Electroporation System. The Neon NxT pulse
generator (9.5 in. W x 7.6 in. H x 9.9 in. D) and Neon NxT pipette station (4.9
in. W x 11.5 in. H x 5.9 in. D) fit inside most BSCs, which helps minimize
sample contamination.







SPECIFICATION COMPARISON BETWEEN THE NEON NXT ELECTROPORATION SYSTEM AND THE
NEON TRANSFECTION SYSTEM

Specification
Neon NxT Electroporation System
Neon Transfection SystemElectroporation Volume10 µL or 100 µL10 µL or 100
µLElectroporation buffer volume*2 mL3 mLTip attachmentClipTip
technologyFrictionElectroporation pulses1-101-10Pulse duration1-100 ms1-100
msPulse voltage500-2,500 V500-2,500 VArc detectionYesNoCloud
connectivityYesNoPulse generator dimensions**9.5 x 7.6 x 9.9 in. (W x H x D)
11. 9 lb (5.4 kg)9.5 x 8.9 x 13.6 in. (W x H x D)
13. 8 lb (6.25 kg)Cable management feature***YesNoTouch display8-inch capacitive
touchscreen7-inch touchscreenElectrical rating100-240 VAC, 270 W100-240 C, 150 W

*The buffer tube of the Neon NxT Electroporation System has a 2 mL level
indicator.
**The Neon NxT pulse generator can be moved into or out of a typical BSC without
removing the sash.
***Excess cable length can be secured behind the Neon NxT system with the
attachable cable organizer.


THE SAME TECHNOLOGY AND PERFORMANCE AS THE NEON TRANSFECTION SYSTEM

Figure 6. Performance of the Neon NxT Electroporation System and the Neon
Transfection System. Performance was evaluated by transfecting different
mammalian cell lines with GFP plasmid DNA or GFP mRNA. (A) GFP plasmid DNA
transfection efficiency reported as the percentage of GFP-positive cells. (B)
Viability of cells after transfection with GFP plasmid DNA. (C) GFP mRNA
transfection efficiency reported as the percentage of GFP-positive cells. (D)
Viability of cells after transfection with GFP mRNA.



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† Kim JA, Cho K, Shin MS, et al. (2008) A novel electroporation method using a
capillary and wire-type electrode. Biosens Bioelectron 23(9):1353–1360.


GIBCO CTS XENON ELECTROPORATION SYSTEM FOR CELL THERAPY MANUFACTURING


A closed, scalable electroporation system for GMP-compliant cell therapy
manufacturing. Process- and protocol-compatible with the Neon NxT Transfection
System, the Xenon system helps enable smooth scale-up from research to process
development to commercial manufacturing of up to 25 mL at a time.

Learn more


Resources

Documents

 * Quick reference guide: Neon NxT Electroporation System
 * Specification Sheet: Neon NxT Electroporation System
 * User Guide: Neon NxT Electroporation System
 * Infographic Flyer: Invitrogen Neon NxT Electroporation System
 * Application note: Achieve exceptional genome editing efficiency with the Neon
   NxT Resuspension Genome Editing Buffer
 * Application note: Streamlining the electroporation workflow with Neon NxT
   Electroporation System: a comparative study with the Neon Transfection System
 * Neon Transfection Instrument Discontinuation Letter


TRANSFECTION PROTOCOLS AND CITATIONS

Search this library to find protocols and citations curated to fit your
experimental needs. Filter by cell type, payload, and product of choice to
easily unlock your answers.
Explore protocols and citations

Cell Culture and Transfection Learning Center
Access cell culture and transfection application resources for more success as
you plan and execute your experiments.

Support
 * Services and Support
 * Visit our Instrument Service Coverage Lookup page to check your existing
   service coverage and renew


TRANSFECTION FAQS

Search our extensive collection of Transfection FAQs to find the answers related
to Neon Electroporation Transfection.

See electroporation FAQs
See Neon system FAQs


SOFTWARE UPDATES

Download the latest software for the Neon NxT Electroporation System

 * Release note
 * Neon NxT Instrument Firmware 1.1.0




For Research Use Only. Not for use in diagnostic procedures.

Stylesheet for Classic Wide Template adjustments


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