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Barcode ID

WELCOME TO BOLD SYSTEMS VERSION 5


IDENTIFYING SPECIES THROUGH DNA BARCODES

BOLDv5 features a completely redesigned layout compared to BOLDv4, with a focus
on richer content and supporting materials. It also includes a more complex site
structure. Tutorials are being developed to facilitate the transition. In the
meantime, BOLDv4 remains accessible at  v4.boldsystems.org 

Learn the Layout


DATA PORTAL


A DATA PORTAL THAT PROVIDES ACCESS TO OVER 16M PUBLIC RECORDS REPRESENTING 1.1M
SPECIES

The records in the portal are public and fully accessible. The remaining records
await validation and release.

Data Portal




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BINS


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ANIMAL SPECIES


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PLANT SPECIES


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FUNGI & OTHER SPECIES



OVERVIEW


PROCESS OF IDENTIFYING SPECIES VIA STANDARDIZED DNA GENETIC MARKERS.

01.
DNA EXTRACTION:

The process begins with isolating DNA from the specimen. This step requires care
to ensure that the genetic material is free from contaminants and adequately
preserved for subsequent analysis.

02.
PCR (POLYMERASE CHAIN REACTION):

Using the extracted DNA, the PCR amplifies target gene regions, creating
numerous copies to facilitate detailed examination. This step is crucial for
enhancing the visibility of genetic markers necessary for identification.

03.
SEQUENCING:

The amplified DNA undergoes sequencing, where the order of nucleotides is
determined. This sequence represents the unique fingerprint of the species and
is essential for accurate identification.

04.
SEQUENCE MATCHING:

Finally, the obtained sequence is matched against reference libraries in BOLD.
This comparison allows for the identification of the specimen by finding the
closest records.


THROUGH THIS METHOD, UNKNOWN SAMPLES ARE IDENTIFIED TO SPECIES PRESENT IN THE
REFERENCE LIBRARY.

Identify Now

DATA


BARCODE INDEX NUMBERS (BINS)

The Barcode Index Number (BIN) system is an automated method for clustering DNA
barcode sequences from animals into operational taxonomic units that usually
correspond to species. Each cluster is assigned a unique identifier which serves
as a proxy for a species, enabling researchers to examine biodiversity patterns
even in the absence of formal species descriptions.

By standardizing DNA-based species identification, the BIN system facilitates
large-scale biodiversity assessments, supports species discovery, and enhances
ecological and conservation efforts.

Each BIN is linked to a dedicated page that consolidates all available data for
its members.

BIN Database

DATA DISSEMINATION


OUR DATABASE PARTNERS

Working with major biodiversity databasing initiatives, BOLD ensures
interoperability and maximizes access to DNA barcode data.



TIMELINE


THE HISTORY OF BOLD

BOLD started as a database and analytical platform but has since expanded its
role. With its rapidly growing database, broad taxonomic coverage, and novel
methods and functionalities, it has become a key component of the biodiversity
informatics landscape.


PROOF-OF-CONCEPT

BOLD was launched as a proof-of-concept platform at the University of Guelph,
designed to manage and disseminate DNA barcode records and standardize DNA
barcoding efforts.



2005




ESSENTIAL TOOL

BOLD2 introduced functionality to support a global network of early adopters of
DNA barcoding. It was accompanied by a publication detailing its storage,
management, and analytical capabilities.



2007




BARCODE INDEX NUMBER

BOLD3 introduced the Barcode Index Number (BIN) system, which improved species
identification and analysis by organizing similar sequences into distinct
clusters and registering them.



2013




EXPANDED CAPABILITIES

BOLD4 was released with advanced analysis tools, a revamped interface, and
expanded marker support.



2016




GLOBAL COLLABORATION AND INNOVATION

BOLD5 introduces a range of new capabilities, including a revamped data model,
support for a network of mirrors, new APIs, improved performance.



2024






GROWTH OF BARCODES & SPECIES ON BOLD




Advancing biodiversity science through DNA-based species identification.

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DATA

 * Portal
 * Data Packages
 * Primers
 * API
 * Taxonomy
 * CAOS
 * BOLDconnectR

RESEARCH TOOLS

 * Workbench
 * Barcode ID

ABOUT

 * Mission
 * Team
 * Sponsors & Partners
 * Citation
 * Contact

RESOURCES HUB

 * FAQ
 * Documentation
 * Standards
 * Templates
 * Communications

© BOLD Systems. All Rights Reserved.

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