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KNOCKIN CELL LINE

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Ubigene has modified over 5000 genes from more than 200 cell lines with our
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Location: Home > Gene Editing Services > Stable Cell Lines > knockin cell line


CRISPR-U™ CELL LINES

CRISPR-U™

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Knockin Cell Lines

iPSC and ESC Gene editing

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method

Overexpression Cell Lines

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CLONING SERVICES

CRISPR

Knockout Plasmid

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VIRUS PACKAGING

· Lentivirus Packaging

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CRISPR LIBRARY

· Customized CRISPR Library

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· Knockout E.coli

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CELL ASSAY

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· Cytokine Secretion and Enzyme Activity Test

· Cytotoxicity Test

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KNOCKIN CELL LINE

The gRNA vector (containing Cas9) of the CRISPR/Cas9 system and the Donor
carrying target mutation or target gene sequences will be co-transfected into
cells by electroporation. Then the gRNA and Cas9 complex will cause a
double-strand break (DSB) on the target site of DNA and the cells will undergo
homologous recombination repair (HDR) using Donor as a template to recombine
target mutations to the target sites. After antibiotic screening, single-cell
clones will be generated. Positive clones that are successfully introduced the
target mutations be validated by target site amplification and sequencing. Final
deliverables will be the positive cell clones, related data and project reports.
Knockin Cell Service Details

Cell type Various types of cells including tumor cell lines, regular cell lines,
IPS/ES cell lines Service type Point Mutation / Reporter Gene Knock-in
Deliverables Cell pool/Single-cell clone Turnaround/Price
8 weeks，as low as $6480   Contact Us

200+Successful
Gene-editing Cell Line Types

Expand All
Respiratory System
+
Chinese hamster lung cells(V79)Human hypopharyngeal carcinoma cell
line(FaDu)Human Bronchial Epithelial Cell Line(16HBE)Human Bronchial Epithelial
Cell Line(BEAS-2B)Human Non-small Cell Lung Carcinoma Cell Line(HCC827)Human
Non-small Cell Lung Carcinoma Cell Line(NCI-H1299)Human Lung Squamous Cell
Carcinoma Cell Line(NCI-H226)Human lung squamous cell carcinoma cell
line(SK-MES-1)Human Lung Cancer Cell Line(NCI-H520)Human Lung Cancer Cell
Line(Calu-1)Human Lung Cancer Cell Line(A549)
Circulatory System
+
Mouse Myoblast Cell Line(C2C12)Human Coronary Artery Endothelial Cell
line(HCAEC)Rat Cardiac Myocytes(HL-1)Rat Cardiac Myocytes(H9C2)
Endocrine System
+
Human Breast Cancer Cell Line(MCF7)Mouse insulinoma β cell line(NIT-1)Human
Breast Cancer Cell Line(JIMT-1)Human breast cancer cell line(T-47D)Human
pancreatic cancer cell line(BxPC-3)Mouse Acinar Pancreatic Cell Line(266-6)Human
Prostate Cancer Cell Line(VCaP)Human Pancreatic Carcinoma Cell Line(MIA
PaCa-2)Mouse medullary breast cancer cell line(E0771)Mouse pancreatic cancer
cell line(Pan02)Human Metastatic Pancreatic Adenocarcinoma Cell
Line(AsPC-1)Human Breast Adenocarcinoma Cell Line(SK-BR-3)Human Pancreatic
Carcinoma Cell Line(PANC-1)Rat Breast Cancer Cell Line(4T1)Human Breast Cancer
Cell Line(ZR-75-1)Human Breast Cancer Cell Line(MDA-MB-231)
Brain and Nervous System
+
Human glioma cell line(U251MG)Mouse microglia cell line(BV2)Immortalize Human
Microvascular Endothelial Cell Line(hCMEC/D3)Mouse Anterior Parietal Bone Cell
Line(MC3T3-E1 Subclone 14)Human glioblastoma cell line(U-87 MG)Rat Glioblastoma
Cell Line(C6)Mouse neuroblastoma cell line(Neuro-2a)Human Neuroblastoma Cell
Line(SK-N-SH)
Blood and lymphatic System
+
Human B lymphoma cell line(Su-DHL-4)Human B Cell Lymphoma Cancer Cell Line
OCI-LY3(OCI-LY3)Human B Cell Lymphoma Cancer Cell Line(U2932)Human Acute Non-B
Non-T Lymphocytic Leukemia Cell Line(Reh)Human myelogenous leukemia cell
line(K-562)Human T lymphocyte cell line(Jurkat, Clone E6-1)Rat Basophil Leukemia
Cell Line(RBL-2H3)Human Monocytic Cell Line(THP-1)Porcine alveolar macrophage
cell line(3D4/21)Mouse Macrophage Cell Line(RAW264.7)
Urinary System
+
Mouse prostate cancer cell line(RM-1)Rat adrenal pheochromocytoma cell
line(PC-12)Distal nephron cell line(Distal nephron cell line（JU4s）)Dog Kidney
Cell Line(MDCK)Human prostate cancer cell line(22RV1)Human Embryonic Kidney Cell
Line(293T)Human Embryonic Kidney Cell Line(HEK293)Human Bladder Transitional
Cell Carcinoma Cell Line(T24)Human bladder carcinoma cell line(5637)Human
bladder carcinoma cell line(TCCSUP)
Digestive System
+
Human colon adenocarcinoma cell line(LS174T)Porcine intestinal epithelial cell
line(IPEC-J2)Human hepatoma cell line(HepaRG)Mouse Hepatocarcinoma Cell
Line(Hepa 1-6)Mouse Hepatocarcinoma Cell Line(H22)Human Hepatoma Cell
Line(SMMC-7721)Human renal carcinoma cell line(ACHN)African green monkey kidney
cell(Vero)Human renal cell carcinoma cell line(786-0)Human Esophageal Squamous
Carcinoma Cell Line(KYSE-30)Human Esophageal Squamous Carcinoma Cell
Line(KYSE-150)Human gastric cancer cell line(AGS)Human Gastric Cancer Cell
Line(SGC-7901)Human Gastric Cancer Cell Line(HGC-27)Human hepatobiliary cancer
cell line(RBE)Human hepatocellular carcinoma cell line(HuH-7)Human Hepatoma Cell
Line(Hep3B)Human liver cancer cell line(Hep G2)Human Normal Hepatocytes Cell
line(L-02)Human colon carcinoma cell line(T84)Human colorectal adenocarcinoma
cell line(Caco-2)Human colorectal adenocarcinoma cell line(NCI-H716)Human colon
adenocarcinoma cell line(DLD-1)Murine colorectal carcinoma cell
line(CT26.WT)Murine Colorectal Carcinoma Cell Line(MC38)Human caucasian colon
adenocarcinoma cell line(COLO 205)Human colon carcinoma cell line(RKO)Human
Colon Cancer Cell Line(HT-29)Human Colon Cancer Cell Line(SW620)Human Colon
Cancer Cell Line(SW480)Human Colon Cancer Cell Line(HCT 116)
Skeleton, Articulus, Soft Tissue, Derma System
+
Mouse osteoid cell line(MLO-Y4)Ameloblastoma(hTERT-AM)Mouse squamous carcinoma
cell line(SCC7)Mouse myeloma cell line(Sp2/0-Ag14)Human skin squamous carcinoma
cell line(A431)Murine melanoma cell line(B16-F10)Human Melanoma Cell
Line(M14)Human malignant melanoma cell line(A-375)Human fibrosarcoma cell
line(HT-1080)Human bone osteosarcoma epithelial cell line(U-2 OS)Human
Osteosarcoma Cell Line(MG-63)
Ocular, Otolaryngologic and Oral System
+
Human retinal pigment epithelial cell line(ARPE-19)Human choroidal melanoma cell
line(OCM-1)Human oral squamous carcinoma cell line(HSC3)Human Nasopharyngeal
Carcinoma Cell Line(C666-1)Human Nasopharyngeal Carcinoma Cell Line(CNE2Z)Human
nasopharyngeal carcinoma cell line(NPC-43)Rat Muller Cell Line(rmc-1)
Reproductive System
+
Human ovarian cancer cell line(OVCAR-3)Human Villous
Trophoblast(HTR-8/SVneo)Human Ovarian Adenocarcinoma Cell Line(CAOV3)Mouse
Testicular Stromal Cell Line(TM3)Mouse Pituitary Cell Line(Lbetat2)Human Ovarian
Cancer Cell Line(SK-OV-3)Chinese Hamster Ovary Cell Line(CHO-K1)Mouse Embryonic
Fibroblasts(NIH/3T3)Human Cervical Carcinoma Cell Line(HeLa 229)Human Cervical
Carcinoma Cell Line(HeLa)
Stem Cell
+
Stem Cell(H9)Human Embryonic Stem Cell(H1)Induced Pluripotent Stem Cell(ipsc)


CRISPR-U™ Technical advantage

Higher cutting and homologous recombination efficiency, 10-fold increase in
gene-editing efficiency

A large number of success case, successfully achieving over 5000 gene edits in
over 200 cell line types

Wide application, easily achieving gene knockout / point mutation / knock-in


Knockin Strategies
Protein fusion
Guide RNA and Cas9 complex cause a double-strand break (DSB) on the target site
of DNA. The donor vector carrying knockin sequence is the template for
homologous recombination repair (HDR), and it recombines to the target site.

Replacement of specific locus:

Work Flow and Validation

Case Study
T cells carrying chimeric antigen receptors (CARs) can mediate tumor rejection,
which is an effective way to cure B cell malignant tumors. By using CRISPR/Cas9
technology, TRAC gene was replaced by CD19 specific CAR sequence.CAR expression
in human peripheral blood T cells was found, and the immune response ability of
T cells was enhanced.
A

CRISPR/Cas9 mediated CAR gene integrated into TARC site.
1928z is a CD19 specific CAR sequence.



B

TCR/CAR flow plots.4 days after co-transfection of
Cas9, gRNA and donor vector, CAR proteins were detected.



Reference：
Eyquem, J., Mansilla-Soto, J., Giavridis, T., van der Stegen, S. J., Hamieh, M.,
Cunanan, K. M., ... & Sadelain, M. (2017). Targeting a CAR to the TRAC locus
with CRISPR/Cas9 enhances tumour rejection. Nature, 543(7643), 113.


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CuineaParaguayPeruPhilippinesPolandFrench PolynesiaPortugalPuerto
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PrincipeSaudi ArabiaSenegalSeychellesSierra
LeoneSingaporeSlovakiaSloveniaSolomon Is.SomaliSouth AfricaSpainSri
LankaSudanSurinameSwazilandSwedenSwitzerlandSyriaTaiwanTajikstanTanzaniaThailandTogoTongaTrinidad
and TobagoTunisiaTurkeyTurkmenistanUgandaUkraineUnited Arab EmiratesUnited
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EMAIL: info@ubigene.com
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TEL: +86 153 6067 3248 (Int'l)
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GENE EDITING SERVICES

 * Stable Cell Lines
 * Knockout
 * Knockdown
 * Overexpression
 * Knockin
 * iPSC and ESC Gene editing
   

 * Cloning Services
 * Knockout
 * Point Mutation
 * Knockin
 * Overexpression
 * Knockdown

 * Virus Packaging
 * Lentivirus Packaging
 * Adenovirus Packaging
 * AAV Packaging

 * CRISPR Library
 * Customized CRISPR Library
 * CRISPR Library In-stock

 * Microbe
 * Gene-editing E.coli
 * Gene-editing Salmonella
 * Gene-editing P. aeruginosa
 * Gene-editing S. aureus


EZ-EDITOR™ PRODUCTS

 * gRNA plasmid bank
 * Cell Lines 3000+
 * Culture Medium
 * Kits
 * Lentivirus
 * IVD positive controls
   

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CONTACT US

  Title： ProfessorExecutive ManagementDirectorPrincipal InvestigatorLab
ManagerStaff ScientistPostdocGraduate StudentResearch
AssociateStudentProcurementOther
  Country/Region： AngolaAfghanistanAlbaniaAlgeriaAndorraAnguillaBarbuda
AntiguaArgentinaArmeniaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermuda
Is.BoliviaBotswanaBrazilBruneiBulgariaBurkina-fasoBurmaBurundiCameroonCanadaCentral
African RepublicChadChileChinaColombiaCongoCook Is.Costa RicaCubaCyprusCzech
RepublicDenmarkDjiboutiDominica Rep.EcuadorEgyptEI
SalvadorEstoniaEthiopiaFijiFinlandFranceFrench
GuianaGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGrenadaGuamGuatemalaGuineaGuyanaHaitiHondurasHongkongHungaryIcelandIndiaIndonesiaIranIraqIrelandIsraelItalyJamaicaJapanJordanKampuchea
(Cambodia
)KazakstanKenyaKoreaKuwaitKyrgyzstanLaosLatviaLebanonLesothoLiberiaLibyaLiechtensteinLithuaniaLuxembourgMacaoMadagascarMalawiMalaysiaMaldivesMaliMaltaMauritiusMexicoMoldovaMonacoMongoliaMontserrat
Is.MoroccoMozambiqueNamibiaNauruNepalNetherlandsNew
ZealandNicaraguaNigerNigeriaNorth KoreaNorwayOmanPakistanPanamaPapua New
CuineaParaguayPeruPhilippinesPolandFrench PolynesiaPortugalPuerto
RicoQatarRomaniaRussiaSaint LueiaSaint VincentSan MarinoSao Tome and
PrincipeSaudi ArabiaSenegalSeychellesSierra
LeoneSingaporeSlovakiaSloveniaSolomon Is.SomaliSouth AfricaSpainSri
LankaSudanSurinameSwazilandSwedenSwitzerlandSyriaTaiwanTajikstanTanzaniaThailandTogoTongaTrinidad
and TobagoTunisiaTurkeyTurkmenistanUgandaUkraineUnited Arab EmiratesUnited
KiongdomUnited States of
AmericaUruguayUzbekistanVenezuelaVietnamYemenYugoslaviaZimbabweZaireZambia
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