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ETHOLOOP

Now in Nature Methods

Accurate tracking and analysis of animal behavior is a crucial element of modern
systems neuroscience. Under naturalistic situations, this can however be very
challenging. The EthoLoop framework is designed to follow freely roaming animals
in natural, three-dimensional (3D) environments, while providing
behavior-triggered stimuli or rewards in a closed-loop manner.







TRACKING IN THE DARK

EthoLoop uses multiple high-speed infrared cameras to track the position of
animals carrying miniature LEDs or retro-reflective markers in the dark. Each
camera interacts with an independent GPU based microprocessor where the 2D
position for each animal is calculated. All 2D coordinates are transmitted
wirelessly to a central computing unit where the 3D position is reconstructed in
real-time.

EthoLoop 3D Tracking



WATCHING CLOSELY


To have continuous close-up views from the tracked animal, the 3D coordinates
are used to control a high-resolution autofocus camera on a motorized gimbal.

EthoLoop Close-Up System



INTERACT WITH THE WILD


EthoLoop controls multiple remote controlled boxes that enable direct
interactions with naive animals in their naturalistic environment. These battery
powered boxes can wirelessly communicate and provide multi-modal stimuli and
liquid reward based on animal’s behavior analysis on the fly. Arbitrarily chosen
behaviors can thus be reinforced within minutes and thus replaces tedious
observations and lengthy animal training. 


EthoLoop RECO-Box


NEXT GENERATION NEUROETHOLOGY


Combining multiple wirelessly controlled reward boxes EthoLoop allows
well-controlled and reproducible behavioral experiments where feedback is
generated on-the-fly, based on real-time behavioral analysis of high resolution
close-up views. Wireless optogenetic manipulations or neurophysiological
recordings can easily be integrated with EthoLoop and thus enable the next
generation neuroethological experiments.




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