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PROTEIN GELS

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‹Protein Gel Electrophoresis
 * Protein Gels
   * Protein Gel Selection Guide
   * Protein Gel Conversion Tool
   * Protein Gels Welcome Packs
   * Novex Tris-Glycine Gels
   * NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels
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Explore our protein gel options. Choose from precast polyacrylamide gel
electrophoresis (PAGE) chemistries designed for specific applications including
broad range, high, or low molecular weight separations, SDS-PAGE, native PAGE,
or IEF. Mini gels and wider, higher throughput, midi gels are offered in a
variety of well and cassette formats. Or select a system for pouring and casting
your own gels.

Find the right gel for your protein
Convert from other suppliers' gels



Protein gels performance guarantee—Invitrogen precast protein gels are designed
and tested to deliver superior performance, reliability, reproducibility, and
consistency of quality. We guarantee it. Check out our  Certificate of Analysis.
No other supplier conducts such stringent quality testing.

Get started with our money-saving Protein Gels Welcome Packs

 * Featured protein gels
 * Compare chemistries
 * IEF and specialized gels
 * Handcast systems


FEATURED PROTEIN GEL CATEGORIES

 
Bis-Tris Gels
Tris-Glycine Gels
Tris-Acetate Gels
Tricine GelsAvailable precast gelsNuPAGE Bis-Tris Gels, Bolt Bis-Tris Plus
GelsNovex Tris-GlycineNuPAGE Tris-AcetateNovex TricineProtein sample typeBroad
range MW (6–400 kDa)Broad range MW (6–400 kDa)High range MW (40–500 kDa)Low
range MW (2.5–40 kDa)Product benefitsNeutral pH helps prevent protein
degradation for high sensitivity. Load up to 60 µL per well with Bolt gels. Fast
20–35 minute run times. Room temperature storage for up to 16 months.
Two buffer options: Choose MOPS buffer for higher range separations or MES
buffer for lower range separations.Traditional Laemmli-style gels with larger
loading capacity—load up to 60 µL per well.Neutral pH for better separation,
transfer, and analysis of high molecular weight proteins.Better separation,
transfer, and analysis of low molecular weight proteins.Recommended forWestern
blotting, in-gel staining, mass spectrometry, post translationally modified
proteins, dilute samples, and denaturing applications.Western blotting, in-gel
staining, samples containing detergent and high salt, and denaturing or native
PAGE applications.High molecular weight proteins, Western blotting, mass
spectrometry, post translationally modified proteins, and denaturing or native
PAGE applications.Low molecular weight proteins, western blotting, in-gel
staining, and denaturing applications.




BIS-TRIS

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeBolt Bis-Tris Plus
 * Time is crtitical
 * Dilute or larger (up to 60 µL) samples 
 * Broad-range separation of proteins
 * Western blot transfer and analysis, and all techniques in which protein
   integrity is crucial

8%
10%
12%
4–12%

6 to 400 kDa
 * Mini with larger well capacity (WedgeWell, up to 60 µL)

22–45 min16 months at room temperatureNuPAGE Bis-Tris
 * Broad-range separation of proteins
 * Western blot transfer and analysis, and all techniques in which protein
   integrity is crucial

8%
10%
12%
4–12%

1.5 to 300 kDa
 * Mini
 * Midi

35–50 min16 months at room temperature


TRIS-GLYCINE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf life

Novex Tris-Glycine

 

 * Broad-range separation of proteins using traditional Laemmli-style gels

6%
8%
10%
12%
4–20%
4–12%
14%
16%
8–16%
10–20%

8 to 250 kDa

 * Mini with larger well capacity (WedgeWell, up to 60 µL)
 * Midi

90 min

12 months


TRIS-ACETATE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeNuPAGE Tris-Acetate
 * Separation of larger proteins

7%
3–8%36 to 500 kDa
 * Mini
 * Midi

60 min8 months


TRICINE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeNovex Tricine
 * Separation of small proteins and peptides

10%
16%2 to 200 kDa
 * Mini

90 min1–2 months


NATIVE BIS-TRIS

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf life

NativePAGE Bis-Tris

 * Separation of native proteins

3–12%
4–16%15 to 10,000 kDa
 * Mini

90–120 min12 months


IEF

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeIEF
 * Separation of proteins based on isoelectric point

pH 3–7
pH 3–10pI 3.5 to pI 8.5
 * Mini

150 min2 months


ZYMOGRAM

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeZymogram
 * Separation of proteins based on size and activity

10% (with gelatin)10 to 220 kDa
 * Mini

90 min2 months


E-PAGE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeE-PAGE
 * High throughput for recombination product analysis and protein profiling

E-PAGE 96: 6%
E-PAGE 48: 8%10 to 220 kDa
 * 48-well
 * 96-well

14–25 min6 months



 
NativePAGE Bis-Tris Gels
View all
Isoelectric Focusing Gels
View all
Novex Zymogram Gels
View all
E-PAGE Precast
Gels
View allGel chemistryBis-TrispH 3–7, pH 3–10Tris-glycine with gelatin8% or
6%Protein sample type15–10,000 kDapI 3.5–8.510–220 kDa10–200 kDaProduct
benefitsSeparation of native proteins, neutral pH for high sensitivity
applicationsSeparation of proteins based on isoelectric pointSeparation of
proteins based on size and activityHigh-throughput gel system, that eliminates
the need for buffers



 
SureCast Handcast System
Explore
Empty Gel Cassettes
Explore
Bolt Empty Cassettes
ExploreCassette typeResuable, glassSingle use, pre-sealedSingle use,
pre-sealedProduct benefits
 * Leak-free design—minimize the number of failed gels, waste less time
 * Superior glass plate durability
 * Unique tilt feature—minimize spillage and confidently load acrylamide
   solutions
 * Simple component assembly—using a single-motion load-and-lock mechanism

 * Ready-to-pour cassettes, casting stand not required, cassettes sealed on
   three sides (no gel leakage)
 * Special coating provides uniform polymerization rate for a more consistent
   handcast gel

 * High capacity sample wells—load up to 60 µL per well
 * Ready-to-pour cassettes, casting stand not required, cassettes sealed on
   three sides (no gel leakage)
 * Special coating provides uniform polymerization rate for a more consistent
   handcast gel

Gel dimensionsMini: 8x8 cm (1 mm thick)

Mini: 8x8 cm (1 or 1.5 mm thick)

Midi: 8x13 cm (1 mm thick)

Mini: 8x8 cm (1 mm thick)Gel tankMini Gel Tank, Xcell SureLock Mini-Cell

Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell

Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)

Mini Gel Tank, Xcell SureLock Mini-CellReagentsSureCast reagents, other popular
polyacrylamide reagentsSureCast reagents, other popular polyacrylamide
reagentsSureCast reagents, other popular polyacrylamide reagentsCombos
available1-, 2-, 10-, 12-, and 15-wells1-, 2-, 5-, 10-, 12-, 15-, and
2D-wells10- or 12-wells (high capacity wells)


Featured protein gels


FEATURED PROTEIN GEL CATEGORIES

 
Bis-Tris Gels
Tris-Glycine Gels
Tris-Acetate Gels
Tricine GelsAvailable precast gelsNuPAGE Bis-Tris Gels, Bolt Bis-Tris Plus
GelsNovex Tris-GlycineNuPAGE Tris-AcetateNovex TricineProtein sample typeBroad
range MW (6–400 kDa)Broad range MW (6–400 kDa)High range MW (40–500 kDa)Low
range MW (2.5–40 kDa)Product benefitsNeutral pH helps prevent protein
degradation for high sensitivity. Load up to 60 µL per well with Bolt gels. Fast
20–35 minute run times. Room temperature storage for up to 16 months.
Two buffer options: Choose MOPS buffer for higher range separations or MES
buffer for lower range separations.Traditional Laemmli-style gels with larger
loading capacity—load up to 60 µL per well.Neutral pH for better separation,
transfer, and analysis of high molecular weight proteins.Better separation,
transfer, and analysis of low molecular weight proteins.Recommended forWestern
blotting, in-gel staining, mass spectrometry, post translationally modified
proteins, dilute samples, and denaturing applications.Western blotting, in-gel
staining, samples containing detergent and high salt, and denaturing or native
PAGE applications.High molecular weight proteins, Western blotting, mass
spectrometry, post translationally modified proteins, and denaturing or native
PAGE applications.Low molecular weight proteins, western blotting, in-gel
staining, and denaturing applications.


Compare chemistries


BIS-TRIS

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeBolt Bis-Tris Plus
 * Time is crtitical
 * Dilute or larger (up to 60 µL) samples 
 * Broad-range separation of proteins
 * Western blot transfer and analysis, and all techniques in which protein
   integrity is crucial

8%
10%
12%
4–12%

6 to 400 kDa
 * Mini with larger well capacity (WedgeWell, up to 60 µL)

22–45 min16 months at room temperatureNuPAGE Bis-Tris
 * Broad-range separation of proteins
 * Western blot transfer and analysis, and all techniques in which protein
   integrity is crucial

8%
10%
12%
4–12%

1.5 to 300 kDa
 * Mini
 * Midi

35–50 min16 months at room temperature


TRIS-GLYCINE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf life

Novex Tris-Glycine

 

 * Broad-range separation of proteins using traditional Laemmli-style gels

6%
8%
10%
12%
4–20%
4–12%
14%
16%
8–16%
10–20%

8 to 250 kDa

 * Mini with larger well capacity (WedgeWell, up to 60 µL)
 * Midi

90 min

12 months


TRIS-ACETATE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeNuPAGE Tris-Acetate
 * Separation of larger proteins

7%
3–8%36 to 500 kDa
 * Mini
 * Midi

60 min8 months


TRICINE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeNovex Tricine
 * Separation of small proteins and peptides

10%
16%2 to 200 kDa
 * Mini

90 min1–2 months


NATIVE BIS-TRIS

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf life

NativePAGE Bis-Tris

 * Separation of native proteins

3–12%
4–16%15 to 10,000 kDa
 * Mini

90–120 min12 months


IEF

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeIEF
 * Separation of proteins based on isoelectric point

pH 3–7
pH 3–10pI 3.5 to pI 8.5
 * Mini

150 min2 months


ZYMOGRAM

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeZymogram
 * Separation of proteins based on size and activity

10% (with gelatin)10 to 220 kDa
 * Mini

90 min2 months


E-PAGE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage
run timeShelf lifeE-PAGE
 * High throughput for recombination product analysis and protein profiling

E-PAGE 96: 6%
E-PAGE 48: 8%10 to 220 kDa
 * 48-well
 * 96-well

14–25 min6 months


IEF and specialized gels

 
NativePAGE Bis-Tris Gels
View all
Isoelectric Focusing Gels
View all
Novex Zymogram Gels
View all
E-PAGE Precast
Gels
View allGel chemistryBis-TrispH 3–7, pH 3–10Tris-glycine with gelatin8% or
6%Protein sample type15–10,000 kDapI 3.5–8.510–220 kDa10–200 kDaProduct
benefitsSeparation of native proteins, neutral pH for high sensitivity
applicationsSeparation of proteins based on isoelectric pointSeparation of
proteins based on size and activityHigh-throughput gel system, that eliminates
the need for buffers


Handcast systems

 
SureCast Handcast System
Explore
Empty Gel Cassettes
Explore
Bolt Empty Cassettes
ExploreCassette typeResuable, glassSingle use, pre-sealedSingle use,
pre-sealedProduct benefits
 * Leak-free design—minimize the number of failed gels, waste less time
 * Superior glass plate durability
 * Unique tilt feature—minimize spillage and confidently load acrylamide
   solutions
 * Simple component assembly—using a single-motion load-and-lock mechanism

 * Ready-to-pour cassettes, casting stand not required, cassettes sealed on
   three sides (no gel leakage)
 * Special coating provides uniform polymerization rate for a more consistent
   handcast gel

 * High capacity sample wells—load up to 60 µL per well
 * Ready-to-pour cassettes, casting stand not required, cassettes sealed on
   three sides (no gel leakage)
 * Special coating provides uniform polymerization rate for a more consistent
   handcast gel

Gel dimensionsMini: 8x8 cm (1 mm thick)

Mini: 8x8 cm (1 or 1.5 mm thick)

Midi: 8x13 cm (1 mm thick)

Mini: 8x8 cm (1 mm thick)Gel tankMini Gel Tank, Xcell SureLock Mini-Cell

Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell

Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)

Mini Gel Tank, Xcell SureLock Mini-CellReagentsSureCast reagents, other popular
polyacrylamide reagentsSureCast reagents, other popular polyacrylamide
reagentsSureCast reagents, other popular polyacrylamide reagentsCombos
available1-, 2-, 10-, 12-, and 15-wells1-, 2-, 5-, 10-, 12-, 15-, and
2D-wells10- or 12-wells (high capacity wells)




POLYACRYLAMIDE GELS

All Invitrogen precast protein gels are high quality polyacrylamide gels
designed to deliver superior performance, reliability, reproducibility, and
consistency of quality. Our extensive portfolio of precast protein gels provides
a wide variety of gel options available for your research. Whether your protein
is 2.5 kDa or as large as 500 kDa, or you’re looking for separation under native
conditions or by isoelectric point—we have the right gel for your application.

Our precast protein polyacrylamide gels are offered in four different
chemistries. The choice of whether to use one chemistry or another depends on
the abundance of the protein you’re separating, the size of the protein, and
your downstream application.


SDS PAGE PROTEIN SEPARATION

For denaturing gel electrophoresis or SDS PAGE there are many options available
based on the application, and size of the protein of interest. For separation of
a broad range of proteins, two chemistries, Bis-Tris and Tris-glycine, have been
optimized for performance and long shelf life. Choose Bis-Tris gel chemistry
when you have a low abundance of protein or when your downstream applications
require high protein integrity, such as posttranslational modification analysis,
mass spectrometry, or sequencing. Bis-Tris chemistry provides a neutral (pH 7.0)
environment during electrophoresis, which may result in better sample integrity
and stability of the gel. This helps reduce protein modifications and helps
produce sharp band resolution. Bis-Tris gel chemistry is offered in our Bolt
Bis-Tris Plus and NuPAGE Bis-Tris gels. Bolt Bis-Tris Plus mini gels build on
the legacy of the highly published NuPAGE Bis-Tris gels, feature larger capacity
wells- for loading up to 60 µl per well, and can be run in as little as 20
minutes.

To separate high-abundance proteins, select our robust Novex Tris-Glycine gel
chemistry, which offers maximum protein separation performance and crisp,
straight bands.

For separation of high molecular weight proteins, Tris-acetate gel chemistry is
recommended, offered in NuPAGE Tris-Acetate gels.

For separation of low molecular weight proteins, use Novex Tricine gels which
provide increased resolution of proteins with molecular weights as low as 2.5
kDa.


NATIVE PAGE PROTEIN SEPARATION

For separation of proteins under nondenaturing conditions or native page, three
gel systems are recommended- NativePAGE Bis-Tris, Novex Tris-Glycine and NuPAGE
Tris-Acetate. NativePAGE Bis-Tris gels, are based on the blue native
polyacrylamide gel electrophoresis (BN-PAGE) which helps overcome the
limitations of traditional native electrophoresis by providing a near-neutral
operating pH and detergent compatibility. Both Novex Tris-Glycine and NuPAGE
Tris-Acetate gels can be run under native and non-reducing conditions for
separation of proteins by net charge, size and shape.

For separation of proteins by isoelectroctric point (pI), use Novex IEF gels.
Novex IEF gels effectively create a pH gradient to separate proteins according
to their unique pI.


HOW-TO VIDEOS

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HOW TO CHOOSE THE RIGHT PROTEIN GEL FOR YOUR RESEARCH APPLICATION

This webinar will highlight the different protein modifications occurring during
electrophoresis, the importance of pH in sample integrity, how to choose the
right gel chemistry for specific research applications, and how to introduce the
right new gel chemistry in your research workflow.



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HOW TO SEPARATE PROTEINS USING AN INVITROGEN PRECAST SDS-PAGE GEL

This video will show you how to separate proteins by using Invitrogen Mini Gel
Tank and Invitrogen precast SDS-PAGE gels.



Need help determining the best gel to use?


PROTEIN GEL SELECTION GUIDE

Find the exact gel and buffers needed for your electrophoresis experiments.


GEL SELECTION CHART

Find the right gel for your research needs based on molecular weight, downstream
applications, and throughput requirements.


PROTEIN GEL OPTIONS BROCHURE

Learn how the right protein gel chemistry can help you achieve better protein
separation and western blots.





PROTEIN GELS PERFORMANCE GUARANTEE

We stand behind the performance of our protein gels.

Learn more


Resources

Download: Protein Gel Guide



Download: Protein gel electrophoresis technical handbook



Download: Western Detection Workflow brochure



Access: Protocols, tips & tricks, and electrophoresis troubleshooting





For Research Use Only. Not for use in diagnostic procedures.


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