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 * Home›
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 * Sanger-Sequenzierung›
 * Sanger Sequencing and Fragment Analysis Software

Maximize the potential of your Sanger sequencing and fragment analysis workflow
with Cloud-based apps ›





SANGER SEQUENCING AND FRAGMENT ANALYSIS SOFTWARE

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‹Sanger-Sequenzierung
 * Sanger Sequencing and Fragment Analysis Software
   * MicrobeBridge Software
   * Minor Variant Finder Software
 * Electrophoresis with Sanger Sequencing ›
 * Sanger Sequencing Reaction Purification ›
 * PCR for Sanger Sequencing ›
 * DNA Isolation for Sanger Sequencing ›
 * Sequencing Reaction for Sanger Sequencing ›

We offer a wide portfolio of Applied Biosystems software solutions for viewing
and interpreting your Sanger sequencing and fragment analysis results.  Learn
about our free tools and commercially available products below.

 * Data Collection Software
 * Sanger sequencing software

 * Primer Design Tool
 * Fragment analysis software

Looking for software support? 
Visit our Capillary Electrophoresis Software Support Center.





DATA COLLECTION SOFTWARE

SoftwareDescriptionOperating system requirements

Data Collection Software

Download software patches ›
Upgrade to the latest version

Data Collection Software is an integrated software for instrument control, data
collection, quality control, base-calling, and size-calling of samples.
Operation of the instrument is dependent on this software.

Windows™ 7
Windows™ 10

SeqStudio Genetic Analyzer software

Download the latest software update ›SeqStudio Genetic Analyzer software is the
embedded software on the SeqStudio instrument.Not ApplicablePlatform for
Science, Sanger Sequencing Solution Supports end-to-end sample tracking from
sample accessioning to sequencing including lineage, volumes and concentrations.
Enables managers to look for bottlenecks in sample processing and lab scientists
to identify samples ready for processing at each step in the process.Platform
for Science


SANGER SEQUENCING SOFTWARE

Viewer

SoftwareDescriptionOperating system requirements

Sequence Scanner Software v2.0

Download ›

Sequence Scanner Software allows you to view, edit, print, and export data
generated using Applied Biosystems genetic analyzer instruments after data has
been processed by Sequencing Analysis Software.

Windows™ 7

Quality Check Module

Sign in ›

The Quality Check (QC) Module evaluates trace quality. The easy-to-interpret
analysis summary gives a snapshot of Sanger sequence trace quality. The Trace
Details page allows you to evaluate and edit traces as needed. The Flag Settings
page allows you to adjust quality thresholds.Free access on Thermo Fisher Cloud

Primary analysis tool

SoftwareDescriptionOperating system requirements

Sequencing Analysis Software

Download software patches ›

Sequencing Analysis Software uses a base-caller algorithm that performs base
calling for pure and mixed base calls.  The software analyzes, displays, edits,
saves, and prints sample files that are generated from Applied Biosystems DNA
analyzers and genetic analyzers.

Windows™ 7
Windows™ 10

Secondary analysis tool

SoftwareDescriptionOperating system requirements

Minor Variant Finder Software

Download trial version ›

Minor Variant Finder Software enables 5% somatic variant detection using Sanger
sequencing. The improved sensitivity makes Sanger sequencing a fast, cost
effective, and accurate way to call low-frequency somatic variants where the
number of relevant targets is limited.

Windows™ 7 SP1, 32-bit or 64-bit or Windows™ 10

SeqScape Software

Download trial version ›

SeqScape Software is a resequencing package designed for mutation detection and
analysis, SNP discovery and validation, pathogen sub-typing, allele
identification, and sequence confirmation. It provides library functions for
comparison to a known group of sequences, as well as features to assist with 21
CFR Part 11 compliance (Security, Audit and electronic signature features),
which can be important in clinical research labs.

Windows™ 7
Windows™ 10

Variant Reporter Software

Download trial version ›
Variant Reporter Software is designed for reference-based and
non-reference-based analysis such as mutation detection and analysis, SNP
discovery and validation, and sequence confirmation. The robust algorithms will
call SNPs, mutations, insertions, deletions, and heterozygous
insertions⁄deletions for data generated using Applied Biosystems genetic
analyzers.

Windows™ 7
Windows™ 10

Variant Analysis (VA) Module

Sign in ›

The Variant Analysis (VA) Module provides fast analysis of Sanger sequencing
data. The VA Module can automatically retrieve reference sequences from the
genomic database, report variants with genomic coordinates, and report genomic
annotations for SNPs. With highly overlapped forward/reverse strands, the VA
Module reports very high sensitivity for SNP calls. The VA Module also reports
and exports variant files in standard .vcf format. There is no software
maintenance required from users.Free access on Thermo Fisher Cloud

Next Generation Confirmation (NGC) Module

Sign in ›

The Next-Generation Confirmation (NGC) Module lets users compare results from
standard NGS variant files with results from Sanger sequencing instruments
within the Thermo Fisher Cloud environment.
Critical decisions often require validation of NGS results using robust Sanger
sequencing. The NGC module provides fast analysis of AB1 files and reports
variants in genomic coordinates. The results are automatically annotated with
known SNPs from the current genomic database.Free access on Thermo Fisher Cloud

MicroSEQ ID Microbial Identification Software

MicroSEQ ID Microbial Identification Software is a tool for identification of
bacteria and fungi. The software analyzes data generated using an Applied
Biosystems MicroSEQ chemistry kit and an Applied Biosystems capillary-based
genetic analyzer.Windows™ 7

MicrobeBridge Software

Download trial version ›

 
MicrobeBridge Software is a streamlined, desktop software solution that connects
DNA sequences generated on Applied Biosystems Sanger sequencers with the Centers
for Disease Control and Prevention (CDC)’s MicrobeNet™ database for bacterial
identification using 16S rRNA gene sequencing analysis. There is no need for
local database setup, so computer resources are easily developed.

Windows™ 7
Windows™ 10

Fragment analysis software
Sizing software

SoftwareDescriptionOperating system requirements

Sizing Analysis Module, Peak Scanner Software

Sign in ›

Peak Scanner module is a DNA fragment sizing software that performs DNA fragment
analysis, separate a mixture of DNA fragments according to their sizes, provide
a profile of the separation, and precisely calculate the sizes of the fragments.
The software allows you to view, edit, analyze, print, and export fragment
analysis data generated using Applied Biosystems genetic analyzers.Free access
on Thermo Fisher Cloud

Microsatellite Analysis Software

Sign in ›

Microsatellite Analysis Software(MSA) is a microsatellite genotyping software
that allows you to analyze a mixture of DNA fragments, separated by size. This
analysis provides a profile of the separation, precisely calculates the sizes of
the fragments, and determines the microsatellite alleles present in the sample.
Microsatellite Analysis is commonly used for Microsatellite Instability in
cancer, Triplet Repeat Expansion in neurodegenerative diseases, Species
Identification & Characterization; and Human Sample Authentication.
Free access on Thermo Fisher Cloud

Peak Scanner Software

Download ›

Peak Scanner Software is a DNA sizing software that can either be downloaded for
free or purchased for free as a software kit.

Use this software to perform DNA fragment analysis, separate a mixture of DNA
fragments according to their sizes, provide a profile of the separation, and
precisely calculate the sizes of the fragments. The software allows you to view,
edit, analyze, print, and export fragment analysis data generated using Applied
Biosystems genetic analyzers. 

Windows™ 7

GeneMapper Software

Download trial version ›
Download software patches ›

GeneMapper Software is a flexible genotyping software package that provides DNA
sizing and quality allele calls for all Applied Biosystems electrophoresis-based
genotyping systems. This software specializes in multi-application
functionality, including Applied Biosystems amplified fragment length
polymorphism (AFLP) analysis, loss of heterozygosity (LOH), microsatellite, and
SNP genotyping analysis. In addition, the security and audit features help users
meet 21 CFR 11 requirements.

Windows™ 7
Windows™ 10

Primer design tool

SoftwareDescriptionOperating system requirements

Primer Designer Tool

Access tool ›

The free, online Primer Designer Tool allows you to search for the right
PCR/Sanger sequencing primer pair from a database of ~650,000 predesigned primer
pairs for resequencing the human exome and human mitochondrial genome.

Web tool

Methyl Primer Express Software v1.0

Download software ›

Methyl Primer Express Software v1.0 enables you to design high-quality PCR
primers for methylation mapping experiments. Simply cut and paste in your region
of interest. The tool searches for CpG islands and simulates bisulfite
modification of DNA in silico. 

NGS confirmation with Sanger sequencing



3500 Series Genetic Analyzers for Sanger sequencing and fragment analysis



Sanger Analysis Modules—free cloud-based tools






RESOURCES

 *  Poster: The first low input, three-day NGS Ion AmpliSeq Methylation Panel
   and protocol
 *  Technical Bulletin: Bisulfite methylation library production and analysis
   using the Ion AmpliSeq Library Kit Plus
 *  Technical Bulleting: Bisulfite methylation library production and analysis
   using the Ion AmpliSeq Kit for Chef DL8

Interested in commercializing your assay?
We can help ›


SUPPORT

 * Next-Generation Sequencing Support Center
   
 * Technical support




For Research Use Only. Not for use in diagnostic procedures.


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