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from QC to gene prediction and phylogenomics

BUSCO v5.6.1 is the current stable version!
Gitlab , a Conda package and Docker container are also available.

Based on evolutionarily-informed expectations of gene content of near-universal
single-copy orthologs, the BUSCO metric is complementary to technical metrics
like N50.

BUSCO was selected as one of the SIB Remarkable Outputs of 2021 !


# CITE US

The latest BUSCO paper, describing the novelties introduced in BUSCO v4 and v5
and the new BUSCO datasets (*_odb10) are described here . If you've used these
versions or datasets the correct citation would be:

Mosè Manni, Matthew R Berkeley, Mathieu Seppey, Felipe A Simão, Evgeny M
Zdobnov, BUSCO Update: Novel and Streamlined Workflows along with Broader and
Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral
Genomes.  Molecular Biology and Evolution, Volume 38, Issue 10, October 2021,
Pages 4647–4654

The following protocol covers the various BUSCO running modes and workflows,
BUSCO setup, guidelines to interpret the results, and additional analyses, e.g.,
for building phylogenomic trees and visualizing syntenies using BUSCO results:

Manni, M., Berkeley, M. R., Seppey, M., & Zdobnov, E. M. (2021). BUSCO:
Assessing genomic data quality and beyond. Current Protocols, 1, e323. doi:
10.1002/cpz1.323

more & stats >>


# LICENSE

The BUSCO software is licensed under the MIT License.

The BUSCO datasets are licensed under the Creative Commons
Attribution-NoDerivatives 4.0 International License. To view a copy of this
license, visit http://creativecommons.org/licenses/by-nd/4.0/ or send a letter
to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.

Any use of these datasets for analyses in a publication or product must include
the citation of the corresponding paper: https://doi.org/10.1093/molbev/msab199
.


# OBTAIN BUSCO

Full installation instructions are provided in the user guide and protocols .

BUSCO is available as a conda package and as a Docker image. Both of these
versions are ready to run out of the box. Alternatively, it is also possible to
manually install BUSCO.

The BUSCO software directly downloads the necessary datasets, whether they are
specified by the user or automatically selected by BUSCO.

To display all available datasets

busco --list-datasets


You can also download them manually .

Earlier versions: v4 , v3 , v2 , v1


# DOCUMENTATION AND SUPPORT

User guide Issues board


# "CORE" GENES

adapted from



EZlab OrthoDB LEMOrtho LEMMI LEMMI16s miRmap NewickUtils
© 2019 EM Zdobnov Group - UNIGE / SIB