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OPTIMIZING MONOCLONAL ANTIBODY PRODUCTION: TEMPERATURE AND OSMOLALITY’S IMPACT
Life Sciences, Pharma Manufacturing & Supply Chain, Drug Discovery &
Development, Laboratory Technology,
*
* Thursday, February 08, 2024 | 11am EST (NA) / 4pm GMT (UK) / 5pm CET
(EU-Central)
* 60 min
* Register
* Description
* Speakers
* Who Should Attend?
* Partners
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Over the past two decades, the biopharmaceutical market has expanded, with
monoclonal antibodies exhibiting a 26 percent higher approval rate than other
drugs. The market value of monoclonal antibodies alone is expected to reach more
than $300 billion USD by 2025. Monoclonal antibodies are recombinant proteins
that are extracellularly secreted by mammalian cells, such as Chinese hamster
ovary (CHO) cells. Advances in monoclonal antibody production technology and
manufacturing strategies have led to increased yield, which is, however,
accompanied by increased levels of impurities, such as host cell proteins
(HCPs).
HCPs are native proteins of CHO cells released in the cell culture supernatant
through secretion or cell lysis upon death. Several factors, such as cell line,
cell culture media components and operating parameters, have been reported to
influence the generation and composition of HCPs in the supernatant. Among
these, culture temperature, osmolality, pH and dissolved oxygen tension can
directly affect cellular behavior, culture viability and, in turn, HCP
accumulation.
Temperature plays an important role in cell culture and can affect
cell growth and cell viability. Meanwhile, osmolality, which generally indicates
the osmotic pressure condition in the cell culture, has a significant impact on
cell growth and metabolism. Studies have reported that manipulating these two
parameters independently could have a direct effect on the concentration of the
HCPs in the supernatant.
Join this webinar to investigate the effects of simultaneously adjusting
temperature and osmolality on the generation of HCPs, antibody yield and cell
growth.
Keywords:
* Antibodies
,
* Drug Development
,
* Antibody
,
* Cell Culture
,
* Monoclonal Antibodies
,
* Laboratory Technology
,
* Recombinant Protein Production
,
* Bioanalytical Testing
,
* Protein
,
* Antibody Drug
,
* Monoclonal Antibody
,
* Osmolality
,
* CDMO/CMO
Register
SPEAKER
SYAZANA MOHAMAD PAUZI, RESEARCH SCIENTIST, IMPERIAL COLLEGE OF LONDON
Syazana Mohamad Pauzi started her PhD research in late 2021 at Imperial College
London under the supervision of Professor Cleo Kontoravdi, investigating the
generation of host cell proteins in the upstream and their subsequent clearance
in the downstream processes. She received her B.Eng in Biotechnology-Biochemical
Engineering from International Islamic University of Malaysia and her M.Eng in
Chemical Engineering from Universiti Kebangsaan Malaysia. Her dissertation
focused on developing and verifying a design advisor tool for monoclonal
antibody production. Syazana previously worked with a biopharmaceutical contract
manufacturing organization in Malaysia before joining academia at UiTM Malaysia.
Her experiences are mostly in the downstream processing of biopharmaceuticals,
which includes filtration and chromatographic operations.
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WHO SHOULD ATTEND?
* Bioprocess scientists
* Cell culture scientists
* Protein production scientists
WHAT YOU WILL LEARN
Attendees will gain insights into:
* Advances in monoclonal antibody production technology and manufacturing
strategies
* Why temperature and osmolality in cell culture are critical parameters,
affecting cell growth, viability and the concentration of HCPs in the
supernatant
* How temperature and osmolality in the cell culture can be optimized, seeking
improved monoclonal antibody production efficiency
XTALKS PARTNER
ADVANCED INSTRUMENTS
Advanced Instruments is a global company offering a novel portfolio of
analytical tools including, OsmoTECH®, a robust line of micro-osmometers to
support bioprocessing and quality control (QC), and Solentim, a portfolio of
best in class imaging and single-cell deposition technologies for cell line
development workflows and assurance of clonality for regulatory bodies.
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