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Berkeley Lab
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ENIGMA

Ecosystems and Networks Integrated with Genes and Molecular Assemblies

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   * ENVIRONMENTAL ATLAS
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ENIGMA – is a consortium of 16 investigators at eleven institutions across the
country led by Lawrence Berkeley Laboratory. Established in 2009, ENIGMA
researchers collaborate to create a predictive model of the impacts of microbial
communities on critical processes within the ecosystem. We study subsurface
microbiomes within the contaminated Bear Creek aquifer at the Oak Ridge
Reservation, a site with complex contaminants, generated by research and
production of nuclear materials, including nitrate, acidity, uranium, technetium
and volatile organic carbon species, the fate of which is influenced by the
activity of subsurface microbial communities. We use sophisticated field
experiments to discover and measure the natural and anthropogenically perturbed
dynamics of geochemical and biological processes. From these we infer the
chemical, physical and microbial interactions that predict these dynamics and
estimate the ecological forces that shape microbial community function. We then
use a unique array of culturing, genetic, physiological and imaging technologies
to capture this diversity in the laboratory and understand the genetic basis for
observed behaviors. Our goal is to provide an unprecedented mechanistic and
predictive understanding of complex environmental bioprocesses with a focus on
pH, oxygen, and metal-mediated carbon, nitrogen and sulfur cycling in the
shallow subsurface of a contaminated watershed.


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