int.diasorin.com Open in urlscan Pro
176.221.48.72  Public Scan

Submitted URL: https://info.diasorin.com/e3t/Ctc/WT+113/bQW-04/MWVJk-1VxTPW36ZwSY6NNRNvW91lZlN5p9rwfN5NMMmd3qgyTW7Y8-PT6lZ3n3W17_-Vx7J9ry...
Effective URL: https://int.diasorin.com/en/luminex-ltg/xmap-technology/microspheres
Submission Tags: falconsandbox
Submission: On December 05 via api from US — Scanned from CA

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IT EN


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   BACK
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 4. XMAP® Microspheres


XMAP® MICROSPHERES


DELIVERING THE VERSATILITY TO PERFORM A WIDE RANGE OF PROTEIN AND NUCLEIC
ACID-BASED MULTIPLEX ASSAYS ON A SINGLE PLATFORM

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xMAP® beads are internally color-coded microspheres with precise concentrations
of multiple fluorescent dyes, yielding up to 500 distinctly colored bead sets.
xMAP® microspheres can be coupled with target-specific molecules such as
antigens, antibodies, oligonucleotides, enzyme substrates, receptors, etc.

After an analyte in a sample is captured by this target-specific molecule, a
fluorescent reporter molecule is introduced to complete the reaction on the
surface of each microsphere.

The internal dyes on the microspheres are excited by the red laser or LED to
identify the microsphere set. Then, a green laser, green LED, or violet laser
(xMAP INTELLIFLEX DR-SE only) excites the fluorescent reporter dye. Using these
signals, each individual microsphere is identified, and the amount of target
molecule bound to its surface is quantified.




XMAP® BEADS



xMAP® beads pass through a red laser, or LED, which excites the internal dyes to
distinguish the microsphere set. Then, green laser, green LED, or violet laser
(xMAP INTELLIFLEX DR-SE only) excites the fluorescent reporter dye to determine
the result of the assay.

Each microsphere contains specific concentrations of internal dyes that
correlate to a specific bead region. Although the internal dyes differ between
regions, the outer coating of carboxyl groups is the same across all bead
regions. This gives customers the flexibility to couple any region of bead to
their analyte of choice, creating endless possibilities for assay development.

xMAP® beads come in a variety of formats, including magnetic (MagPlex) and
non-magnetic (MicroPlex) beads.


XMAP MAGPLEX® MICROSPHERES



MagPlex® Microspheres—6.5-micron superparamagnetic beads that are dyed with
three red and infrared fluorescent dyes, resulting in 500 distinctly colored
bead sets—are our most versatile and efficient microspheres for high-plex
applications. Approximately 108 surface carboxyl groups (COOH) cover the surface
of the bead and serve as covalent attachments for capturing ligands. A
fluorescent reporter is coupled to a target molecule, which allows its detection
after specific capture on the microsphere surface.

What can be coupled to the beads?

 * Antibodies
 * Antigens/Other Proteins
 * Modified Oligos

 * Peptides
 * Carbohydrates
 * Small Molecules

ORDERING INFO

Find out more about the available beads, as well as all the information
necessary to submit your order.

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XMAP® APPLICATIONS

Explore xMAP® Technology and discover some of the many applications used by
multiplexing research experts across the globe.

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LUMINEXPLORE LAB

Looking for custom assay design and development services with unique targets in
mind? Discover the LuminexPLORE Lab and partner with the xMAP® multiplexing
industry experts.

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XMAP® KIT FINDER

xMAP® Technology is an open multiplexing platform used in hundreds of commercial
immunoassay kits. Industry leading manufacturers offer xMAP Technology assay
kits. To find them, use our xMAP® Kit Finder.

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