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* RosetteSep™
ROSETTESEP™ IMMUNODENSITY CELL ISOLATION AND CELL SEPARATION
ROSETTESEP™ IMMUNODENSITY CELL SEPARATION
ISOLATE CELLS FROM WHOLE BLOOD DURING DENSITY GRADIENT CENTRIFUGATION
RosetteSep™ is a fast and easy immunodensity procedure for the isolation of
untouched cells directly from whole blood. By crosslinking unwanted cells to red
blood cells (RBCs) present in the sample, target cells are purified during
standard density gradient centrifugation (e.g. peripheral blood mononuclear cell
(PBMC) isolation using Lymphoprep™ , Ficoll-Paque™, RosetteSep™ DM-L Density
Medium or RosetteSep™ DM-M Density Medium. This approach significantly reduces
sample handling time and maximizes convenience by incorporating cell subset
isolation into the centrifugation process.
HOW DOES ROSETTESEP™ WORK?
* Unwanted cells are crosslinked to RBCs by specific antibodies forming dense
immunorosettes.
* Immunorosettes pellet during density gradient centrifugation, leaving
untouched and purified target cells at the interface between the plasma and
the density gradient medium.
* No columns or magnets are necessary. Just incubate, spin, and collect
purified cells.
View All RosetteSep™-Containing Cell Separation Products >
HOW TO ISOLATE CELLS DIRECTLY FROM BLOOD WITH ROSETTESEP™
To isolate purified cell subsets from whole blood, simply incubate your sample
with the appropriate RosetteSep™ cocktail for 20 minutes. Next, layer the sample
on a density gradient medium (e.g. Lymphoprep™, Ficoll-Paque™) and centrifuge
for 20 minutes. After centrifugation is complete, collect the untouched cell
subset from the interface between the plasma and the density gradient medium.
Request a Sample >
WHY USE ROSETTESEP™?
* FAST AND EASY. Isolate untouched cell subsets from whole blood during your
standard density gradient centrifugation step.
* VERSATILE. Choose from a wide range of RosetteSep™ reagents to isolate the
cell subset of your interest.
* HIGHLY VIABLE AND FUNCTIONAL CELLS. Obtain highly purified, unlabeled and
viable cells that are immediately ready for downstream analysis.
* VERSATILE. Combine RosetteSep™ with SepMate™ PBMC isolation tubes to minimize
variability between separations.
RosetteSep™ for Human Cells Density Gradient Media RosetteSep™ Protocol Combine
RosetteSep™ with SepMate™ Key Applications
ROSETTESEP™ FOR HUMAN CELL ISOLATION
View All Products
ROSETTESEP™
TYPICAL SAMPLE SOURCE:
Whole Blood* or Bone Marrow
PROTOCOL TIME:
As Little As 25 Minutes
SELECTION METHOD:
Negative Selection
RELATED PRODUCTS
* Density media: See table below
* Tubes: SepMate™
FEATURES/ADVANTAGES:
* Column-free
* Cells are purified during standard density gradient centrifugation
* Untouched cells are immediately ready for downstream analysis
* Can be combined with SepMate™
View Products
Request a Sample >
DENSITY GRADIENT MEDIA
View All Products
LYMPHOPREP™
DENSITY
1.077 g/mL
FOR ENRICHMENT OF
Mononuclear Cells (MNCs)
TYPICAL SAMPLE SOURCE
Peripheral Whole Blood*, Cord Blood, Bone Marrow
COST-EFFECTIVE ALTERNATIVE TO FICOLL® COMPATIBLE WITH
* All RosetteSep™ cocktails
* SepMate™
View Product
ROSETTESEP™ DM-L DENSITY MEDIUM
DENSITY
1.081 g/mL
FOR ENRICHMENT OF
Human Lymphocyte Populations
TYPICAL SAMPLE SOURCE
Peripheral Whole Blood*
RECOMMENDED FOR
Isolation using the following RosetteSep™ cocktails:
Human T Cell Enrichment (Catalog #15021)
Human CD4+ T Cell Enrichment (Catalog #15022)
Human CD8+ T Cell Enrichment (Catalog #15023)
Human B Cell Enrichment (Catalog #15024)
Human Total Lymphocyte Enrichment (Catalog #15223)
HLA Lymphoid Cell Enrichment Kit (Catalog #15271HLA)
Use with SepMate™
View Product
ROSETTESEP™ DM-M DENSITY MEDIUM
DENSITY
1.085 g/mL
FOR ENRICHMENT OF
Human Myeloid Cells
TYPICAL SAMPLE SOURCE
Peripheral Whole Blood*
RECOMMENDED FOR
Isolation using the following RosetteSep™ cocktail:
HLA Myeloid Cell Enrichment Kit (Catalog #15272HLA)
Use with SepMate™
View Product
Expand Details
Collapse Details
*Certain products are only available in select territories. Please contact your
local Sales representative or the Product & Scientific Support team at
techsupport@stemcell.com for further information.
THE TYPICAL ROSETTESEP™ CELL ENRICHMENT PROTOCOL
1. Add RosetteSep™ Enrichment Cocktail to whole blood and incubate for 20
minutes (10 minutes if using SepMate™ ). Dilute sample with an equal volume
of PBS + 2% FBS and mix gently.
2. Layer the diluted sample on top of the density medium. Use SepMate™ to
prevent the layers from mixing.
3. Centrifuge for 20 minutes at 1200 x g at room temperature, with the brake
off. Use SepMate™ to reduce centrifugation time to 10 minutes with the brake
on.
4. Remove the enriched cells from the density medium : plasma interface. Wash
enriched cells with PBS + 2% FBS. Repeat.
COMBINE ROSETTESEP™ WITH SEPMATE™ FOR ONE-STEP CELL ISOLATION
RosetteSep™ is easily combined with SepMate™ PBMC isolation tubes to increase
sample throughput and eliminate user errors associated with improper sample
layering or PBMC harvesting. This approach allows users with minimal training to
consistently perform cell isolation by density gradient centrifugation and is an
ideal fit for a busy laboratory environment.
Learn more about SepMate™ >
KEY APPLICATIONS
CELL ISOLATION DIRECTLY FROM WHOLE BLOOD
The NK Cell Cytotoxicity Case Study discusses a publication from Dr. Ajay Jain’s
lab at the University of Maryland School of Medicine, which used the RosetteSep™
and SepMate™ system to reduce NK cell isolation time from four hours to a single
hour for a 450 mL unit of blood.
ANTIBODY FINDER TOOL
FIND THE RIGHT ANTIBODY FOR YOUR APPLICATIONS
Don't waste valuable research time finding the appropriate antibody for your
cell analysis. Use our Antibody Finder Tool to select high-quality primary and
secondary antibodies verified to work with RosetteSep™ products in specific
applications.
View Tool >
RELATED RESOURCES
OVERVIEW OF CELL SEPARATION
Learn everything you need to know about cell separation.
Read Now >
ISOLATE CELLS FROM BLOOD
Learn about various methods to isolate different cell populations from blood
samples.
Learn More >
Protocol
How to Isolate Specific Cell Subsets by Immunodensity Cell Separation
Tech Tip
Comparing Cell Separation Platforms from STEMCELL Technologies
Technical Bulletin
High-Throughput Immunodensity-Based Cell Subset Isolation Directly from Whole
Blood
SELECTED REFERENCES USING ROSETTESEP™
See publications using RosetteSep™ >
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