www.artiencegroup.com Open in urlscan Pro
151.101.2.132 Public Scan

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Submitted URL:
https://schd.toyoinkgroup.com/ja/rd/casestudy/probe.html
Effective URL:
https://www.artiencegroup.com/ja/products/rd/fluorescence-probe.html
Submission: On May 18 via manual (May 18th 2024, 7:42:42 pm UTC) from US — Scanned from JP

Form analysis
11 forms found in the DOM

https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html

<form action="https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html">
  <fieldset>
    <legend class="visuallyhidden"><!--wovn-src:Consent Selection-->Consent Selection</legend>
    <div id="CybotCookiebotDialogBodyFieldsetInnerContainer">
      <div class="CybotCookiebotDialogBodyLevelButtonWrapper"><label class="CybotCookiebotDialogBodyLevelButtonLabel" for="CybotCookiebotDialogBodyLevelButtonNecessary"><strong
            class="CybotCookiebotDialogBodyLevelButtonDescription"><!--wovn-src:Necessary-->Necessary</strong></label>
        <div class="CybotCookiebotDialogBodyLevelButtonSliderWrapper CybotCookiebotDialogBodyLevelButtonSliderWrapperDisabled"><input type="checkbox" id="CybotCookiebotDialogBodyLevelButtonNecessary"
            class="CybotCookiebotDialogBodyLevelButton CybotCookiebotDialogBodyLevelButtonDisabled" disabled="disabled" checked="checked"> <span class="CybotCookiebotDialogBodyLevelButtonSlider"></span></div>
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      <div class="CybotCookiebotDialogBodyLevelButtonWrapper"><label class="CybotCookiebotDialogBodyLevelButtonLabel" for="CybotCookiebotDialogBodyLevelButtonPreferences"><strong
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      <div class="CybotCookiebotDialogBodyLevelButtonWrapper"><label class="CybotCookiebotDialogBodyLevelButtonLabel" for="CybotCookiebotDialogBodyLevelButtonStatistics"><strong
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            data-target="CybotCookiebotDialogBodyLevelButtonStatisticsInline" checked="checked" tabindex="0"> <span class="CybotCookiebotDialogBodyLevelButtonSlider"></span></div>
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      <div class="CybotCookiebotDialogBodyLevelButtonWrapper"><label class="CybotCookiebotDialogBodyLevelButtonLabel" for="CybotCookiebotDialogBodyLevelButtonMarketing"><strong
            class="CybotCookiebotDialogBodyLevelButtonDescription"><!--wovn-src:Marketing-->Marketing</strong></label>
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      </div>
    </div>
  </fieldset>
</form>

https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html

<form action="https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html"><input type="checkbox" id="CybotCookiebotDialogBodyLevelButtonNecessaryInline" class="CybotCookiebotDialogBodyLevelButton CybotCookiebotDialogBodyLevelButtonDisabled"
    disabled="disabled" checked="checked"> <span class="CybotCookiebotDialogBodyLevelButtonSlider"></span></form>

https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html

<form action="https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html"><input type="checkbox" id="CybotCookiebotDialogBodyLevelButtonPreferencesInline"
    class="CybotCookiebotDialogBodyLevelButton CybotCookiebotDialogBodyLevelConsentCheckbox" data-target="CybotCookiebotDialogBodyLevelButtonPreferences" checked="checked" tabindex="0"> <span class="CybotCookiebotDialogBodyLevelButtonSlider"></span>
</form>

https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html

<form action="https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html"><input type="checkbox" id="CybotCookiebotDialogBodyLevelButtonStatisticsInline"
    class="CybotCookiebotDialogBodyLevelButton CybotCookiebotDialogBodyLevelConsentCheckbox" data-target="CybotCookiebotDialogBodyLevelButtonStatistics" checked="checked" tabindex="0"> <span class="CybotCookiebotDialogBodyLevelButtonSlider"></span>
</form>

https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html

<form action="https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html"><input type="checkbox" id="CybotCookiebotDialogBodyLevelButtonMarketingInline"
    class="CybotCookiebotDialogBodyLevelButton CybotCookiebotDialogBodyLevelConsentCheckbox" data-target="CybotCookiebotDialogBodyLevelButtonMarketing" checked="checked" tabindex="0"> <span class="CybotCookiebotDialogBodyLevelButtonSlider"></span>
</form>

https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html

<form class="CybotCookiebotDialogBodyLevelButtonSliderWrapper" action="https://www.artiencegroup.com/en/products/rd/fluorescence-probe.html"><input type="checkbox" id="CybotCookiebotDialogBodyContentCheckboxPersonalInformation"
    class="CybotCookiebotDialogBodyLevelButton"> <span class="CybotCookiebotDialogBodyLevelButtonSlider"></span></form>

GET https://www.artiencegroup.com/en/search.html

<form action="https://www.artiencegroup.com/en/search.html" class="c-searchBox02__form" data-form-category="product" method="get" data-wovn-link-src-href="https://www.artiencegroup.com/ja/search.html">
  <div class="c-searchBox02__formContent"><input id="q2" name="q" placeholder="Search by keyword" type="search" autocomplete="off" data-wovn-src:-original-placeholder="キーワードで探す">
    <div class="poplink pcia poplink_invisible" style="position: absolute; left: 0px;">
      <div class="poplink_suggest poplink_invisible">
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
      </div>
      <div class="poplink_search poplink_invisible">
        <div class="header"><!--wovn-src:商品検索-->Product Search</div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
      </div>
      <div class="poplink_recommend poplink_invisible">
        <div class="header"><!--wovn-src:おすすめ-->recommendation</div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
      </div>
    </div><input type="submit" value="">
  </div>
  <input type="hidden" name="ssr">
</form>

GET https://www.artiencegroup.com/en/search.html

<form action="https://www.artiencegroup.com/en/search.html" class="c-searchBox01__form" method="get" data-wovn-link-src-href="https://www.artiencegroup.com/ja/search.html">
  <div class="c-searchBox01__formContent"><input id="q1" name="q" type="search" autocomplete="off">
    <div class="poplink pcia poplink_invisible" style="position: absolute; left: 0px;">
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        <div class="word poplink_invisible"></div>
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        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
      </div>
      <div class="poplink_search poplink_invisible">
        <div class="header"><!--wovn-src:商品検索-->Product Search</div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
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          <div class="userarea"></div>
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          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
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      <div class="poplink_recommend poplink_invisible">
        <div class="header"><!--wovn-src:おすすめ-->recommendation</div>
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          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
      </div>
    </div><input type="submit" value="">
  </div>
</form>

GET https://www.artiencegroup.com/en/search.html

<form action="https://www.artiencegroup.com/en/search.html" class="l-header__searchForm c-searchBox02__form" method="get" data-wovn-link-src-href="https://www.artiencegroup.com/ja/search.html">
  <div class="l-header__searchFormContent c-searchBox02__formContent"><input id="q1Sp" name="q" placeholder="Search by keyword" type="search" autocomplete="off" data-wovn-src:-original-placeholder="キーワードで探す">
    <div class="poplink pcia poplink_invisible" style="position: absolute; left: 0px;">
      <div class="poplink_suggest poplink_invisible">
        <div class="word poplink_invisible"></div>
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        <div class="word poplink_invisible"></div>
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        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
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      <div class="poplink_search poplink_invisible">
        <div class="header"><!--wovn-src:商品検索-->Product Search</div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
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      <div class="poplink_recommend poplink_invisible">
        <div class="header"><!--wovn-src:おすすめ-->recommendation</div>
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          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
      </div>
    </div><input type="submit" value="">
  </div>
</form>

GET https://www.artiencegroup.com/en/search.html

<form action="https://www.artiencegroup.com/en/search.html" class="p-localNav__searchBoxForm c-searchBox02__form" data-form-category="product" method="get" data-wovn-link-src-href="https://www.artiencegroup.com/ja/search.html">
  <div class="p-localNav__searchBoxFormContent c-searchBox02__formContent"><input id="q3" name="q" placeholder="Search by keyword" type="search" autocomplete="off" data-wovn-src:-original-placeholder="キーワードで探す">
    <div class="poplink pcia poplink_invisible" style="position: absolute; left: 0px;">
      <div class="poplink_suggest poplink_invisible">
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
        <div class="word poplink_invisible"></div>
      </div>
      <div class="poplink_search poplink_invisible">
        <div class="header"><!--wovn-src:商品検索-->Product Search</div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
      </div>
      <div class="poplink_recommend poplink_invisible">
        <div class="header"><!--wovn-src:おすすめ-->recommendation</div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
        </div>
        <div class="item">
          <a><div class="item_left"><div class="image_box"><img></div></div><div class="item_right"><div class="title"></div><div class="summary"></div><div class="any1"></div><div class="any2"></div><div class="any3"></div></div><div class="clear"></div></a>
          <div class="userarea"></div>
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      </div>
    </div><input type="submit" value="">
  </div>
  <input type="hidden" name="ssr">
</form>

POST https://www.artiencegroup.com/en/inquiry/

<form action="https://www.artiencegroup.com/en/inquiry/" class="p-contact01__btnGroupItem" method="post" data-wovn-link-src-href="https://www.artiencegroup.com/ja/inquiry/">
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  <input name="company" type="hidden" value="artience株式会社">
  <input name="url" type="hidden" value="https://www.artiencegroup.com/ja/products/rd/fluorescence-probe.html">
</form>

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 * Near-infrared fluorescent probe for high-sensitivity imaging


NEAR-INFRARED FLUORESCENT PROBE FOR HIGH-SENSITIVITY IMAGING

Sciforiem ™ FI Series

A near-infrared fluorescent probe for high-sensitivity imaging that enables
observation of minute areas and long periods of time in regenerative medicine
technology and cancer research.

 * Development product overview
 * Application example
 * Technology overview


DEVELOPMENT PRODUCT OVERVIEW

With the development of regenerative medicine technology in recent years, cell
observation technology has expanded to include high-sensitivity imaging to
observe finer areas, long-term observation of dynamics, and in-vivo cell
observation using near-infrared light, which has high bio-penetration. - Many
new observation systems, such as protein dynamics observation, are being
researched.

The colorants used in these new observation systems not only have optical
properties such as light absorption and fluorescence emission, but also have
clearer labels, long-term luminescence, and in-vivo properties that were not
found in conventional biolabeling colorants. New features such as stability and
resistance to high-power lasers are required.

This developed product has high fluorescence intensity, uniform cytoplasmic
labeling, and excellent photostability, and is suitable for high-content
analysis used in drug discovery screening and long-term observation with
super-resolution microscopy using high-power lasers. , enables finer observation
of biological tissues such as cells.



Appearance of developed fluorescent color material


FEATURES OF THE DEVELOPED PRODUCT

FEATURE 1: HIGH FLUORESCENCE INTENSITY (OBSERVATION USING A FLUORESCENCE
MICROSCOPE *1*2)

Due to its high fluorescence intensity, advanced imaging is possible with a
small amount of additives.

Existing product A

Development product

FEATURE 2 HOMOGENEOUS LABELING OF CELLS (OBSERVATION USING A FLUORESCENCE
MICROSCOPE *1 *2)

Individual cells can be labeled quickly and uniformly.

Existing product A

Development product

*1 When HeLa cells are labeled using near-infrared fluorescent dye
*2 Light in the near-infrared region cannot be recognized by the human eye, so
it is visualized in green.

FEATURE 3: HIGH PHOTOSTABILITY

Because it has high photostability, it is difficult to decompose and shows
stable absorbance even under conditions of strong energy irradiation such as in
super-resolution microscopy.
It also has excellent durability, allowing long-term fluorescence observation.


APPLICATION EXAMPLE

 * cell labeling
 * deep imaging
 * Exosome imaging


APPLICATION EXAMPLE 1 CELL LABELING (MULTIPLE LABELING)

More advanced multiplexed labeling is possible because it uses fluorescence in
the near-infrared region, which has a different wavelength from the visible
light fluorescent colorant.

INTRODUCING STAINED EXOSOMES INTO HELA CELLS AND OBSERVING THEM USING A
SUPER-RESOLUTION CONFOCAL LASER MICROSCOPE (IN COLLABORATION WITH NIKON CO.,
LTD.)



*For convenience, the colors in the image are visually displayed in green, blue,
and red, depending on the fluorescence wavelength emitted by each coloring
material.


APPLICATION EXAMPLE 2 DEEP IMAGING

It exhibits high fluorescence intensity in the wavelength range that is highly
permeable to living organisms, making it possible to image deep parts of living
organisms. Additionally, by taking advantage of this feature, it is possible to
observe minute areas such as capillaries, lymph vessels, and cancer tumor sites.

IN VIVO NEAR-INFRARED BIOFLUORESCENCE IMAGING (INTRAVENOUS INOCULATION, EX720NM,
EM795NM) *3



*3 Ex represents excitation wavelength and Em represents fluorescence detection
wavelength.


APPLICATION EXAMPLE 3 EXOSOME IMAGING

Because it labels exosomes without aggregating them, accurate observation of
exosome dynamics is possible.
Exosomes were introduced into all cells, indicating that exosomes labeled with
this developed product have excellent cellular uptake.

INTRODUCING STAINED EXOSOMES INTO HELA CELLS AND OBSERVING THEM USING A
SUPER-RESOLUTION CONFOCAL LASER MICROSCOPE (IN COLLABORATION WITH NIKON CO.,
LTD.)

Magnification 60x
Existing product B

Magnification 60x
Development product

Magnification 210x
Existing product B

Magnification 210x
Development product

Green (exosome): Existing product B (Ex:561nm, Em582-618nm) *3
Blue (nucleus): Hoechst33258 (Ex:405nm,Em430-463nm nm) *3

*3 Ex represents excitation wavelength and Em represents fluorescence detection
wavelength.



APPLICATION EXAMPLE 4 OTHERS

It can be applied to gene amplification fluorescent labels, cell surface
analysis fluorescent labels, identification of differentially expressed
proteins, quantitative markers, immunohistological staining markers for
pathological examinations, and fluorescent microparticles for in vitro
diagnostics.


TECHNOLOGY OVERVIEW

Colorant absorption/fluorescence wavelength control technology x
Cell-interacting functional group introduction technology

The main light-absorbing substances that exist in living organisms are water
(more than 2000 nm) and hemoglobin in blood (less than 700 nm), so light in the
near-infrared region with a wavelength of 700 nm to 2000 nm is not absorbed by
these substances. , penetrate deeply into living tissues. At our company, we
have combined the "colorant absorption/fluorescence wavelength control
technology" cultivated in the display and electronics fields with our unique
"cell-interacting functional group introduction technology" to achieve clearer
labeling functions, long-term luminescence maintenance, and We are developing
colorants with characteristics suitable for new observation systems, such as
in-vivo stability and photostability.
We aim to apply this coloring material not only to regenerative medicine but
also to a wide range of fields such as biopharmaceutical screening and in vitro
diagnostic systems.

Near-infrared region with high biological permeability

Source: Ministry of Education, Culture, Sports, Science and Technology, Council
for Science and Technology, Resource Survey Subcommittee Report


COLORANT ABSORPTION/FLUORESCENCE WAVELENGTH CONTROL TECHNOLOGY

To achieve absorption/fluorescence in the near-infrared region, the energy of
the highest energy bonding orbital (HOMO: Highest Occupided Molecular Orbital)
and the lowest energy antibonding orbital (LUMO: Lowest Unoccupided Molecular
Orbital) of the coloring material is determined. We designed the color material
to reduce the difference. One way to reduce these energy differences is to
increase the HOMO energy level by, for example, introducing an electron-donating
group into the coloring material.


TECHNOLOGY FOR INTRODUCING CELL-INTERACTING FUNCTIONAL GROUPS

By combining the color material design technology we have cultivated in various
fields with a chemical structure that strongly interacts with biological
materials, we are able to achieve "high fluorescence intensity," "uniform
labeling," and "excellent photostability." We have developed a near-infrared
fluorescent colorant for cell labeling that has the following properties.

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