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Submission: On March 19 via manual from US — Scanned from ES
Submission: On March 19 via manual from US — Scanned from ES
Form analysis 9 forms found in the DOM
Name: searchForm — GET /search.html
<form method="get" action="/search.html" onsubmit="return checkSearch()" id="searchForm" name="searchForm">
<input class="search" type="text" id="searchParam" name="searchDTO.searchParam" autocomplete="off">
<input type="hidden" name="sp" id="sp">
<button class="search" id="subSearch" type="submit">搜 索</button>
</form>Name: sizelist — POST /cart/addCart.jhtml
<form id="prodaddfrom" action="/cart/addCart.jhtml" method="post" name="sizelist">
<table id="stock_status">
<tbody>
<tr>
<th width="120">规格 </th>
<th width="90">价格</th>
<th width="70">库存</th>
<th width="80">购买数量</th>
<th width="387"> </th>
</tr>
<tr>
<td colspan="4" valign="top">
<table>
<tbody>
<tr>
<td width="130">
<span class="in_bg">10mM (1mL in DMSO)</span>
</td>
<td width="100"> RMB 1278.83 </td>
<td width="70">现货</td>
<td width="80">
<select name="quantity_61_10mM/1mL_1278.83">
<option value="0">0</option>
<option value="1">1</option>
<option value="2">2</option>
<option value="3">3</option>
<option value="4">4</option>
<option value="5">5</option>
<option value="6">6</option>
<option value="7">7</option>
<option value="8">8</option>
<option value="9">9</option>
<option value="10">10</option>
</select>
</td>
</tr>
<tr>
<td width="130">
<label>2mg</label>
</td>
<td width="100"> RMB 576.16 </td>
<td width="70">现货</td>
<td width="80">
<select name="quantity_61_2mg_576.16">
<option value="0">0</option>
<option value="1">1</option>
<option value="2">2</option>
<option value="3">3</option>
<option value="4">4</option>
<option value="5">5</option>
<option value="6">6</option>
<option value="7">7</option>
<option value="8">8</option>
<option value="9">9</option>
<option value="10">10</option>
</select>
</td>
</tr>
<tr>
<td width="130">
<label>5mg</label>
</td>
<td width="100"> RMB 972.96 </td>
<td width="70">现货</td>
<td width="80">
<select name="quantity_61_5mg_972.96">
<option value="0">0</option>
<option value="1">1</option>
<option value="2">2</option>
<option value="3">3</option>
<option value="4">4</option>
<option value="5">5</option>
<option value="6">6</option>
<option value="7">7</option>
<option value="8">8</option>
<option value="9">9</option>
<option value="10">10</option>
</select>
</td>
</tr>
<tr>
<td width="130">
<label>10mg</label>
</td>
<td width="100"> RMB 1396.58 </td>
<td width="70">现货</td>
<td width="80">
<select name="quantity_61_10mg_1396.58">
<option value="0">0</option>
<option value="1">1</option>
<option value="2">2</option>
<option value="3">3</option>
<option value="4">4</option>
<option value="5">5</option>
<option value="6">6</option>
<option value="7">7</option>
<option value="8">8</option>
<option value="9">9</option>
<option value="10">10</option>
</select>
</td>
</tr>
<tr>
<td width="130">
<label>50mg</label>
</td>
<td width="100"> RMB 4674.45 </td>
<td width="70">现货</td>
<td width="80">
<select name="quantity_61_50mg_4674.45">
<option value="0">0</option>
<option value="1">1</option>
<option value="2">2</option>
<option value="3">3</option>
<option value="4">4</option>
<option value="5">5</option>
<option value="6">6</option>
<option value="7">7</option>
<option value="8">8</option>
<option value="9">9</option>
<option value="10">10</option>
</select>
</td>
</tr>
<tr>
<td width="130">
<label>大包装</label>
</td>
<td width="100"> 有超大折扣 </td>
<td colspan="2">
<div id="qqchatProduct" class="widget_qq fr" style="margin-right: 43px;">
<img src="https://file.selleck.cn/2015cn/images/icon-qq-chat.png">
</div>
</td>
</tr>
</tbody>
</table>
</td>
<td>
<div class="OF_hidden">
<input type="button" class="button_addToCart fl" value="加入购物车" onclick="addToCartBasket()">
<a class="button_bulkInquiry fl ml6" href="/Y-27632-bulk-inquiry.html" rel="nofollow">大剂量询单有大折扣!</a>
</div>
<p class="clear">
<strong>今日订购，明日送达，全国免运费！<br><br>全国免费电话：400-668-6834 | Email：<a href="mailto:info@selleck.cn" rel="nofollow">info@selleck.cn</a></strong>
</p>
</td>
</tr>
</tbody>
</table>
</form>Name: molCalc —
<form name="molCalc" id="molCalc">
<ul>
<li>
<div style="float:left;width:223px"><strong>质量</strong></div>
<div style="float:left;width:223px"><strong>浓度</strong></div>
<div style="float:left;width:223px"><strong>体积</strong></div>
<div><strong>分子量</strong></div>
</li>
<li>
<div class="fl">
<input class="cal_input" type="text" name="mass">
<select name="MassUnits" class="cal_unit">
<option value="-12">pg</option>
<option value="-9">ng</option>
<option value="-6">μg</option>
<option selected="" value="-3">mg</option>
<option value="0">g</option>
<option value="3">kg</option>
</select>
<label class="cal_operator">=</label>
</div>
<div class="fl">
<input class="cal_input" type="text" name="concentration">
<select name="ConcUnits" class="cal_unit">
<option value="-15">fM</option>
<option value="-12">pM</option>
<option value="-9">nM</option>
<option value="-6">μM</option>
<option selected="" value="-3">mM</option>
<option value="0">M</option>
</select>
<label class="cal_operator red">×</label>
</div>
<div class="fl">
<input class="cal_input" type="text" name="volume">
<select name="VolUnits" class="cal_unit">
<option value="-9">nL</option>
<option value="-6">μL</option>
<option selected="" value="-3">mL</option>
<option value="0">L</option>
</select>
<label class="cal_operator red">×</label>
</div>
<div class="fl">
<input class="cal_input" type="text" value="" name="MolWt">
</div>
</li>
</ul>
</form>Name: dilCalc —
<form name="dilCalc" id="dilCalc">
<ul>
<li>
<div style="float:left;width:223px"><strong>C1</strong></div>
<div style="float:left;width:223px"><strong>V1</strong></div>
<div style="float:left;width:223px"><strong>C2</strong></div>
<div><strong>V2</strong></div>
</li>
<li>
<div class="fl">
<input class="cal_input BG_orange" type="text" name="concentrationStart">
<select name="ConcUnitsStart" class="cal_unit">
<option value="-15">fM</option>
<option value="-12">pM</option>
<option value="-9">nM</option>
<option value="-6">μM</option>
<option selected="selected" value="-3">mM</option>
<option value="0">M</option>
</select>
<label class="cal_operator red">×</label>
</div>
<div class="fl">
<input class="cal_input BG_blue" id="dilVol1Bg" name="volumeStart">
<select name="VolUnitsStart" class="cal_unit">
<option value="-9">nL</option>
<option value="-6">μL</option>
<option selected="selected" value="-3">mL</option>
<option value="0">L</option>
</select>
<label class="cal_operator">=</label>
</div>
<div class="fl">
<input class="cal_input BG_orange" type="text" name="concentrationFinal">
<select name="ConcUnitsFinal" class="cal_unit">
<option value="-15">fM</option>
<option value="-12">pM</option>
<option value="-9">nM</option>
<option value="-6">μM</option>
<option selected="selected" value="-3">mM</option>
<option value="0">M</option>
</select>
<label class="cal_operator red">×</label>
</div>
<div class="fl">
<input class="cal_input BG_orange" type="text" value="" name="volumeFinal">
<select name="VolUnitsFinal" class="cal_unit">
<option value="-6">μL</option>
<option selected="selected" value="-3">mL</option>
<option value="0">L</option>
</select>
</div>
</li>
<li class="clear"><input type="button" class="mt10" onclick="CalcDilute(ffCompat(dilCalc)); return false" value="Calculate"></li>
</ul>
</form>Name: serialCalc —
<form name="serialCalc" id="serialCalc">
<h2>连续稀释计算器方程</h2>
<div class="cal_tool">
<ul class="fl S_form">
<li>
<h3>连续稀释</h3>
</li>
<li><label>初始浓度:</label> <input name="concentrationInit" value="c0"></li>
<li><label>稀释倍数:</label> <input value="x" name="dilutionfold"></li>
<li><label> </label> <input type="button" class="button_commom mt10" onclick="CalcSerialDilute(ffCompat(serialCalc)); return false" value="Calculate"></li>
</ul>
<ul class="fr">
<li>
<h3>计算结果</h3>
</li>
<li>
<table class="data_sheet_frame">
<tbody>
<tr>
<th width="100px;">C1=C0/X</th>
<td width="40px">C1: </td>
<td width="200px" id="concentration1"></td>
<td width="60px">LOG(C1): </td>
<td width="200px" id="logcon1"></td>
</tr>
<tr>
<th width="100px;">C2=C1/X</th>
<td width="40px">C2: </td>
<td width="200px" id="concentration2"></td>
<td width="60px">LOG(C2): </td>
<td width="200px" id="logcon2"></td>
</tr>
<tr>
<th width="100px;">C3=C2/X</th>
<td width="40px">C3: </td>
<td width="200px" id="concentration3"></td>
<td width="60px">LOG(C3): </td>
<td width="200px" id="logcon3"></td>
</tr>
<tr>
<th width="100px;">C4=C3/X</th>
<td width="40px">C4: </td>
<td width="200px" id="concentration4"></td>
<td width="60px">LOG(C4): </td>
<td width="200px" id="logcon4"></td>
</tr>
<tr>
<th width="100px;">C5=C4/X</th>
<td width="40px">C5: </td>
<td width="200px" id="concentration5"></td>
<td width="60px">LOG(C5): </td>
<td width="200px" id="logcon5"></td>
</tr>
<tr>
<th width="100px;">C6=C5/X</th>
<td width="40px">C6: </td>
<td width="200px" id="concentration6"></td>
<td width="60px">LOG(C6): </td>
<td width="200px" id="logcon6"></td>
</tr>
<tr>
<th width="100px;">C7=C6/X</th>
<td width="40px">C7: </td>
<td width="200px" id="concentration7"></td>
<td width="60px">LOG(C7): </td>
<td width="200px" id="logcon7"></td>
</tr>
<tr>
<th width="100px;">C8=C7/X</th>
<td width="40px">C8: </td>
<td width="200px" id="concentration8"></td>
<td width="60px">LOG(C8): </td>
<td width="200px" id="logcon8"></td>
</tr>
</tbody>
</table>
</li>
</ul>
</div>
</form>Name: entryForm —
<form name="entryForm" id="entryForm">
<div>
<input type="text" value="C10H16N2O2" onblur="if(this.value==''){this.value='C10H16N2O2';this.className='input_text input_focus';}" name="inputFormula">
<input type="button" name="" onclick="writeOut(true)" value="Calculate">
</div>
</form>Name: outputForm —
<form name="outputForm" id="outputForm">
<div>
<h3>总分子量：<input type="text" readonly="readonly" id="outputMM">g/mol</h3>
</div>
</form>Name: molCalc1 —
<form name="molCalc1" id="molCalc1">
<table width="data_sheet_frame">
<tbody>
<tr>
<td>质量</td>
<td>浓度</td>
<td>体积</td>
<td>分子量</td>
</tr>
<tr>
<td> <input type="text" name="mass" class="cal_input" style="width:75px;">
<select name="MassUnits" class="cal_unit">
<option value="-12">pg</option>
<option value="-9">ng</option>
<option value="-6">μg</option>
<option selected="" value="-3">mg</option>
<option value="0">g</option>
<option value="3">kg</option>
</select>
<label class="cal_operator">=</label>
</td>
<td> <input type="text" name="concentration" class="cal_input" style="width:55px;">
<select name="ConcUnits" class="cal_unit">
<option value="-15">fM</option>
<option value="-12">pM</option>
<option value="-9">nM</option>
<option value="-6">μM</option>
<option selected="" value="-3">mM</option>
<option value="0">M</option>
</select>
<label class="cal_operator red">×</label>
</td>
<td> <input type="text" name="volume" class="cal_input" style="width:55px;">
<select name="VolUnits" class="cal_unit">
<option value="-9">nL</option>
<option value="-6">μL</option>
<option selected="" value="-3">mL</option>
<option value="0">L</option>
</select>
<label class="cal_operator red">×</label>
</td>
<td><input type="text" value="320.26" name="MolWt" class="cal_input" style="width:100px;"></td>
</tr>
<tr>
<td colspan="1"><a href="#######" onclick="CalcMolarity(molCalc1); return false" class="button_commom">计算</a></td>
<td colspan="3" width:="240px" height="80px"" class="pt5">
<img class="fr" id="weixinAPP" src="https://file.selleck.cn/images/applet.png">
</td>
</tr>
</tbody>
</table>
</form><form id="revQuestion">
<ul class="S_form">
<li>
<span class="red">* 必填项</span>
</li>
<li><label><em>* </em>姓名：</label>
<input type="text" name="questionDTO.cusName" class="input :name">
<input type="hidden" name="questionDTO.type" value="3">
<input type="hidden" name="questionDTO.product" value="S1049 Y-27632 2HCl">
</li>
<li><label><em>* </em>邮箱：</label>
<input type="text" name="questionDTO.cusEmail" class="input :email">
</li>
<li><label> 电话：</label>
<input type="text" name="questionDTO.cusTel" class="input">
</li>
<li><label><em>* </em>留言内容：</label>
<textarea name="questionDTO.content" class=":required"></textarea>
</li>
<li><label> </label><input class="submit" name="" type="submit" value="提交"></li>
</ul>
</form>Text Content
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* Y-27632 2HCl
Y-27632 2HCL
For research use only. Not for use in humans.
目录号：S1049
CAS No. 129830-38-2
Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂，无细胞试验中Ki为140
nM，比对其他激酶包括PKC，cAMP依赖性蛋白激酶，MLCK和PAK的作用强200多倍。
规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1278.83 现货 0 1 2 3 4 5 6 7 8 9 10 2mg RMB 576.16 现货 0 1 2
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客户使用SELLECK生产的Y-27632 2HCL发表文献799篇：
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PubMed: 26483550 ( click the link to review the publication )
* Rejuvenation Res, 2015, 18(6):517-27
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产品安全说明书
批次： S104927 S104928 S104929 S104930 S104926 纯度： 99.58 %
99.58 % <1
* Nature medicine文章证明Selleck质量第一
* COA
* NMR
* HPLC
* Microanalysis (为什么要做元素分析？)
* SDS
* Datasheet
99.45 % 12
* Nature medicine文章证明Selleck质量第一
* COA
* NMR
* HPLC
* Microanalysis (为什么要做元素分析？)
* SDS
* Datasheet
98.99 % 11
* Nature medicine文章证明Selleck质量第一
* COA
* NMR
* HPLC
* Microanalysis (为什么要做元素分析？)
* SDS
* Datasheet
98.98 % 8
* Nature medicine文章证明Selleck质量第一
* COA
* NMR
* HPLC
* Microanalysis (为什么要做元素分析？)
* SDS
* Datasheet
98.76 % 15
* Nature medicine文章证明Selleck质量第一
* COA
* NMR
* HPLC
* Microanalysis (为什么要做元素分析？)
* SDS
* Datasheet
99.58
ROCK抑制剂选择性比较
抑制剂名称 ROCK ROCK1 ROCK2 其他靶点 Y-27632 2HCl ++ + Thiazovivin + Fasudil (HA-1077)
HCl + Calcium channel,Rho,PKA GSK429286A +++ ++ RKI-1447 +++ +++ Y-27632 ++ +
H-1152 dihydrochloride +++ CaMK2,PKG,Aurora A Azaindole 1 (TC-S 7001) ++++ ++++
Hydroxyfasudil (HA-1100) HCl + + PKA Y-39983 HCl ++++ Netarsudil (AR-13324) 2HCl
++++ norepinephrine transporter (NET) GSK269962A HCl ++++ ++++ MSK1,RSK1
Ripasudil (K-115) hydrochloride dihydrate ++ ++ Belumosudil (KD025) ++ AT13148
+++ ++++ PKA,p70S6K,Akt1 Emetine hydrochloride √ BRD4,RhoA,PDK1
点击展开此表
Notes: 1. 鼠标悬停在“+”上可以显示相关IC50的具体数值
2. 更多细节，例如半抑制浓度（IC50）以及任何抑制剂的相关工作浓度，请点击您感兴趣抑制剂的链接。
3. "+"代表抑制作用的强度。"+"越多，抑制作用越强。
4. 橙色的"√"表示该化合物对相应的亚型有抑制作用，但抑制强度暂时没有相关数据。
生物活性
产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂，无细胞试验中Ki为140
nM，比对其他激酶包括PKC，cAMP依赖性蛋白激酶，MLCK和PAK的作用强200多倍。 靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay) ROCK2 [6]
(Cell-free assay) 140 nM(Ki) 300 nM(Ki)
体外研究
Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性，Ki分别为26
μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化，而抑制多种兴奋剂而不是KCl，包括Phenylephrine,
Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩，IC50为0.3-1
μM。Y-27632 作用于培养的细胞，抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理，阻断
Rho调节的肌动球蛋白的激活，也阻断LPA刺激的MM1 细胞入侵活性，这种作用存在浓度依赖性。[2] 10 μM Y-27632
处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES)，显著减少分离诱导的凋亡，提高克隆效率(从~1%提高到~27%),
转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity
Description PMID Salivary gland stem cells Function Assay 10 µM 7 d Reduces SGSC
senescence 25804560 HT22 Cytotoxic Assay 10 µM 13 h Protects against
glutamate-induced neuronal death 22810835 Swiss3T3 Colony-forming Assay 10 µM 13
d Increases prostate cell colony-forming activity 21464902
... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID Western Blot
p-LIMK1(Thr508);
PubMed: 24704720 Gastroenterology
Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected
transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated
with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with
antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.
p-LIMK2(Thr505);
PubMed: 24704720 Gastroenterology
Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected
transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated
with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with
antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.
p-Cofilin(Ser3);
PubMed: 24704720 Gastroenterology
Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected
transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated
with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with
antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.
CDK2;
PubMed: 26555939 PLoS One
Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was
remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells
cultured without Y-27632 in the same condition are used as control. β-ACTIN
serves as an endogenous control.
KRT7;
PubMed: 26555939 PLoS One
Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was
remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells
cultured without Y-27632 in the same condition are used as control. β-ACTIN
serves as an endogenous control.
ROCK2;
PubMed: 26555939 PLoS One
Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was
remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells
cultured without Y-27632 in the same condition are used as control. β-ACTIN
serves as an endogenous control.
E-cadherin;
PubMed: 27399334 Oncogene
The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets
in SW-480-FOXM1D cells treated with Y-27632.
p-MLC2(Ser19);
PubMed: 27399334 Oncogene
The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets
in SW-480-FOXM1D cells treated with Y-27632.
24704720 26555939 27399334 Transwell migration assay
cell migration inhibition ;
PubMed: 27694793 Med Sci Monit
Data represent mean ± SEM, n=3. Compared with control, * P<0.05, ** P<0.01.
Compared with control, Y-27632, or EGCG, *** P<0.01.
27694793 Immunofluorescence
Neurotoxicity Assay;
PubMed: 21362567 Cell Stem Cell
Representations of neurodegeneration in H9-, HUF5- and G2019S-iPSC derived
neurons when treated with neurotoxins, H2O2 and MG-132, and ROCK inhibitor
Y-27632. Scale bar = 50 µm.
E-cadherin;
PubMed: 24523903 PLoS One
The localized patterns of E-cadherin and b-catenin were examined using specific
antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24
hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity.
beta-catenin;
PubMed: 24523903 PLoS One
The localized patterns of E-cadherin and b-catenin were examined using specific
antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24
hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity.
Phalloidin;
PubMed: 27399334 Oncogene
Triple IF staining for F-actin (phalloidin, green) E-cadherin (b) (red) and
nuclei (DAPI, blue) showed decreased F-actin, increased E-cadherin and altered
cell shape and size in SW-480-FOXM1D cells treated with the ROCKs inhibitor
Y-27632 or fasudil (both 10 μM, for 24 h). Scale bar, 25 μm.
21362567 24523903 27399334 Growth inhibition assay
cell proliferation ;
PubMed: 24523903 PLoS One
MCF-7 cells and MDA-MB-231 cells were cultured on tissue culture plates for four
days with or without Y-27632 (20 µM). Relative cell proliferation rates were
determined by the cell number assayed using CCK-8 kit. The data are expressed as
the mean ± SD. n = 3 culture dishes. The p-value was less than 0.05 for
comparisons between control (Control) and treatment groups (Y-27632) in MCF-7
cells. *, p<0.05.
24523903 ELISA
caspase-9;
PubMed: 30320378 Int J Oncol
Caspase-9 activity was measured via ELISA. Mst1-mediated casapse-9 activation
was inhibited by Y-27632 in A549 cells. *P<0.05. Ad, adenovirus; Mst1, mammalian
STE20-like kinase 1.
30320378
体内研究 Y-27632按30
mg/kg剂量口服处理自发性高血压大鼠，肾性高血压大鼠，及去氧皮质酮醋酸(DOCA)盐高血压大鼠，显著降低血压，这种作用存在剂量依赖性。[1]Y-27632按每小时0.55
μL通过植入泵持续处理表达Val14-RhoA的大鼠，持续11天，延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环，通过抑制ROCK, 降低
缺氧诱导的血管生成，和血管重构。[4]
推荐的实验操作(此推荐来自于公开的文献所以SELLECK并不保证其有效性)
动物实验：[1] [7]
- 合并
* Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
* Dosages: 30 mg/kg/day (大鼠)；0-10 mg/kg （小鼠）
* Administration: 口服处理 (大鼠)；ip（小鼠）
(Only for Reference)
参考文献
* [1] Uehata M, et al. Nature, 1997, 389(6654), 990-994.
* [2] Itoh K, et al. Nat Med, 1999, 5(2), 221-225.
* [3] Bito H, et al. Neuron, 2000, 26(2), 431-441.
* [4] Watanabe K, et al. Nat Biotechnol, 2007, 25(6), 681-686.
* [5] Hyvelin JM, et al. Circ Res, 2005, 97(2), 185-191.
* [6] Ishizaki T, et al. Mol Pharmacol. 2000, 57(5), 976-983.
* [7] Isler D, et al. Pharmacol Rep. 2014, 66(1), 114-120.
- 合并
溶解度 (25°C)
体外
批次（可选）： S104927 S104928 S104929 S104930 S104926 DMSO (warmed with 50ºC water
bath) 64 mg/mL (199.84 mM) Water 64 mg/mL (199.84 mM) Ethanol Insoluble 体内
从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)：
saline
10mg/mL
* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。
化学数据 DOWNLOAD Y-27632 2HCL SDF
分子量 320.26 化学式
C14H21N3O.2HCl
CAS号 129830-38-2 储存条件 3年-20°C 粉状
2年-80°C 溶于溶剂 别名 N/A
动物体内配方计算器 (澄清溶液)
第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） 给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量 只
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方） % DMSO+ %
PEG300 Corn oil + % Tween 80+ % ddH2O %DMSO+ % Corn oil PEG300 计算重置
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH2O，混匀澄清。
体内配方配制方法：取 μL DMSO母液，加入 μL Corn oil，混匀澄清。
注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
制备储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg 1 mM 3.1225 mL 15.6123 mL 31.2246 mL 5 mM 0.6245 mL
3.1225 mL 6.2449 mL 10 mM 0.3122 mL 1.5612 mL 3.1225 mL 50 mM 0.0624 mL 0.3122
mL 0.6245 mL
计算器
摩尔浓度计算器
摩尔浓度计算器
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:
质量 (MG) = 浓度 (MM) X 体积 (ML) X 分子量 (G/MOL)
摩尔浓度计算公式
* 质量
浓度
体积
分子量
* pg ng μg mg g kg =
fM pM nM μM mM M ×
nL μL mL L ×
*在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。
稀释计算器
稀释计算器
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 X 开始体积 = 最终浓度 X 最终体积
稀释公式
稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )
* C1
V1
C2
V2
* fM pM nM μM mM M ×
nL μL mL L =
fM pM nM μM mM M ×
μL mL L
*
在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.
连续稀释计算器方程
* 连续稀释
* 初始浓度:
* 稀释倍数:
*
* 计算结果
* C1=C0/X C1: LOG(C1): C2=C1/X C2: LOG(C2): C3=C2/X C3: LOG(C3): C4=C3/X C4:
LOG(C4): C5=C4/X C5: LOG(C5): C6=C5/X C6: LOG(C6): C7=C6/X C7: LOG(C7):
C8=C7/X C8: LOG(C8):
分子量计算器
分子量计算器
通过输入化合物的化学式来计算其分子量：
总分子量：G/MOL
注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2
摩尔浓度计算器
质量 浓度 体积 分子量 pg ng μg mg g kg = fM pM nM μM mM M × nL μL mL L × 计算
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常见问题及建议解决方法
* 问题 1：
Is there any data about the Amax (maximum attraction luminosity) and
extinction coefficient of this compound?
* 回答：
The wavelength we used to test HPLC is 260nm while the extinction coefficient
is unknown.
* 问题 2：
Could this product be used in cell culture? Do you have any reference for
this application?
* 回答：
Yes. The Y-27632 can be used in cell culture certainly. Here is the reference
website: http://molpharm.aspetjournals.org/content/57/5/976.full.
ROCK SIGNALING PATHWAY MAP
ROCK INHIBITORS WITH UNIQUE FEATURES
* 选择性强的Rock抑制剂
Fasudil (HA-1077) HCl : ROCK-II-selective, Ki=0.33 μM.
* 活性最高的ROCK抑制剂
GSK429286A : ROCK1, IC50=14 nM; ROCK2, IC50=63 nM.
* 用于临床的ROCK抑制剂
Fasudil (HA-1077) HCl : Phase III for scleroderma.
相关ROCK产品
* Ro-3306 New
RO-3306是一种ATP竞争性的选择性 CDK1 抑制剂，Ki 为20 nM，选择性是其他各种人类激酶的15倍多。RO-3306可增强p53介导的
Bax 激活和线粒体的凋亡。
* ML141 New
ML141 (CID-2950007) 是 Rho 家族GTPase cdc42的一种有效的，选择性可逆非竞争性抑制剂，IC50为200
nM。对cdc42比对其他Rho家族中的GTPases(如Rac1, Rab2, Rab7)更有选择性。 ML141 与 p38
激活增加有关，并可能诱导p38依赖性凋亡/衰老。ML141还可保护神经母细胞瘤细胞免受二甲双胍(metformin)诱导的凋亡。
* Salirasib New
Salirasib (Farnesylthiosalicylic acid, FTS)是一种有效的竞争性prenylated protein
methyltransferase (PPMTase)抑制剂， Ki为2.6 μM，其抑制Ras甲基化。Salirasib
具有抗肿瘤的活性并可诱导凋亡。Phase 2。
* Thiazovivin
Thiazovivin是一种新型ROCK抑制剂，无细胞试验中IC50为0.5 μM，在单细胞分离后，促进人胚胎干细胞（hESC）的存活。
* GSK429286A
GSK429286A (RHO-15)是一种选择性的ROCK1和ROCK2抑制剂，IC50分别为14 nM和63 nM。
Features:More selective than Y-27632.
* Fasudil (HA-1077) HCl
Fasudil (HA-1077, AT-877)是一种有效的、具有选择性的Rho抑制剂（ROCK2, Ki=0.33
μM），同时对PKA、PKG、PKC和MLCK也具有抑制活性，Ki值分别为1.6 μM、1.6 μM、3.3 μM、36 μM。Fasudil
也是calcium channel的阻滞剂。
* Palbociclib (PD-0332991) HCl
Palbociclib (PD-0332991) HCl是一种高度选择性的CDK4/6抑制剂，无细胞试验中IC50分别为11 nM/16
nM。它对CDK1/2/5，EGFR，FGFR，PDGFR，InsR等没有抑制活性。Phase 3。
Features:PD-0332991作为有应用前景的工具测定CDK激酶是否具有明显的治疗价值。
* Alisertib (MLN8237)
Alisertib (MLN8237)是一种选择性Aurora A抑制剂，无细胞试验中IC50为1.2 nM，作用于Aurora A比作用于Aurora
B选择性强200倍以上。Alisertib 可诱导细胞周期阻滞、细胞凋亡与自噬。Phase 3。
Features:MLN8237 是第一个口服有效的小分子Aurora A激酶选择性抑制剂。
* Adavosertib (MK-1775)
Adavosertib (MK-1775, AZD1775)是一种有效的，高选择性Wee1抑制剂，无细胞试验中IC50为5.2
nM；阻碍G2期DNA损伤检验点。Phase 2。
Features:First reported Wee1 inhibitor
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity
Description PMID