www.rndsystems.com Open in urlscan Pro
2.17.188.35  Public Scan

Form analysis
3 forms found in the DOM

Name: et_searchbar — GET /search

<form name="et_searchbar" method="GET" action="/search" class="search_form">
  <div class="et_searchbar">
    <input autocomplete="off" name="keywords" type="text" class="et_searchbar_wide et_searchbar_default ui-autocomplete-input" value="" placeholder="Search keyword, molecule name, target, catalog number, or product type" data-default=""
      spellcheck="false"><span role="status" aria-live="polite" class="ui-helper-hidden-accessible"></span><input type="submit" value="" class="search_btn fp_mag_glass_wideSearch" style="float: none;">
  </div>
</form>

GET /search

<form action="/search" class="form-inline" method="get" id="faceted_search_bar">
  <div class="form-group keyword-input">
    <input type="hidden" name="category" value="Secondary Antibodies">
    <input id="pc_facet" type="textfield" name="keywords" placeholder="Keywords">
  </div>
  <input type="submit" name="submit" value="Search" id="pc_submit" class="btn btn-primary">
</form>

POST

<form id="fileUploadForm" enctype="multipart/form-data" method="post" target="fileUploadIframe"><input type="file" id="fileSelector" name="file" style="display: none;"><input name="filename" type="hidden"></form>

Text Content

Skip to main content

My Account sign in / register
 * Sign In
 * Register New Account
 * 
   Change Country
 * Quick Order
   Add to Order
   Add Multiple Items
   

R&D Systems uses cookies to provide you with a great website experience. By
continuing to use this website you acknowledge this and agree to our cookie
policy. Learn More
I Agree
 * 0 Items

 * Products
   
   * Products by Category
     
     * Recombinant Proteins
     * GMP Proteins
     * Antibodies
     * ELISAs
     * CometAssay
     * Luminex Assays
     * Luminex Instruments
     * Cell Culture
     * Stem Cell Products
     * Small Molecules & Peptides
     * Enzymes
     * ELISpots
     * cDNA Clones
     * Flow Cytometry Workflow Solutions
     * Proteome Profiler Antibody Arrays
   * Products by Research Area
     
     * Cell Therapy
     * Immunotherapy
     * Cancer
     * Immunology
     * Neuroscience
     * Apoptosis and Cell Viability
     * Biological Activity Assays
     * Glycobiology
     * Cardiovascular Biology
     * Ubiquitin
   * 
     Our Products
 * Services
   
   * All Custom Services
   * Custom Protein Services
   * Luminex Custom Services
   * Custom Antibody Services
   * Assay Custom Services
   * Custom Services for Cell & Gene Therapies
   * Genome Engineering Services
   * Bioactivity Testing Services
   * Chemistry Services
   * Bulk/Custom Formulation
   * Custom cDNA Cloning
   
   * EXPEDITE YOUR RESEARCH
     
     Expedite Your Research
   * Good Manufacturing Practice (GMP) Antibody Services
 * Pathways
   
   * Cancer
   * Cardiovascular Biology
   * Development
   * Glycobiology
   * Immunology
   * Neuroscience
   * Proteases & Other Enzymes
   * Signal Transduction
   * Stem Cell
   * Ubiquitin, Related Modifiers, & Pathways
   * View All Pathways
   
   * AWARD-WINNING INTERACTIVE PATHWAYS
     
     Explore Our Interactive Pathways
 * Resources
   
   * Research Areas
   * Protocols/Troubleshooting Guides/Videos
   * Technical Information
   * Articles
   * Help & FAQs
   * Literature
   * Request Literature
   * Calculators
   * Posters
   * Blog
   * Promotions
   * Travel Grants
   
   * POSTERS, BROCHURES, WHITEPAPERS & REFERENCE GUIDES
     
     Request Literature
   * Cell Markers
   * New Lab Program
 * R&D Systems Quality
   
   * Quality Philosophy
   * People
   * Place
   * Performance
   * Product Development
   * Customer Satisfaction
   * The R&D Systems Quality Advantage
   * Documents, Certifications, & FAQs
   
   * EXPERTS IN REAGENT QUALITY
     
     R&D Systems Quality
 * About Us
   
   * Careers
   * Bio-Techne Brands
   * Investor Relations
   
   * OUR PEOPLE ARE OUR GREATEST ASSET
     
     Join Us
 * Contact Us

Home / Products / Introduction to Secondary Antibodies


INTRODUCTION TO SECONDARY ANTIBODIES

SEARCH SECONDARY ANTIBODIES



Secondary antibodies are a powerful tool that can help amplify signal and
increase sensitivity in an assay. Since multiple secondary antibodies are able
to detect a primary antibody, signal is increased and in turn creates a more
sensitive assay. Secondary antibodies can also provide flexibility in multiple
labeling experiments by having the ability to pick from a variety of diverse
conjugation options to fit the specific needs of the assay. 

R&D Systems offers over 90 different secondary antibody options specific for a
variety of species and conjugated to fluorescent and chromogenic labels. Our
secondary antibodies have been validated in-house for use in Flow Cytometry,
Immunohistochemistry, Immunocytochemistry, and Western blotting.

Reactivity Target IgG Class Conjugates anti-Mouse IgG Biotin anti-Goat IgM
Allophycocyanin (APC) anti-Rabbit IgY Phycoerythrin (PE) anti-Rat IgG2A HRP
anti-Sheep IgG2B Fluorescein anti-Chicken IgE NorthernLights™ 493 anti-Hamster  
NorthernLights™ 557 anti-Human   NorthernLights™ 637     PerCP

View all Secondary Antibodies

 



CONTENTS



 * What Is A Secondary Antibody?
 * How to Select A Secondary Antibody
 * VisUCyte™ HRP Polymer

 * NorthernLights™ Secondary Antibodies and Streptavidin Conjugates
 * Additional Antibody Resources



 



WHAT IS A SECONDARY ANTIBODY?



A secondary antibody is an antibody that is used to bind the immunoglobulin
(IgG) domain of the primary antibody. The secondary antibody will be specific to
the primary antibody’s species and isotype. A secondary antibody is usually
conjugated with a molecule that allows for the antibody to be detected. The
molecules can be chromogenic or fluorescent.





 



HOW TO SELECT A SECONDARY ANTIBODY



When selecting a secondary antibody, it should be chosen based off the primary
antibody and have the appropriate conjugate for the application it is being used
in. Since the secondary antibody is used to detect the primary antibody, the
secondary antibody should detect the host species of the primary antibody. For
instance, if the primary antibody is a goat anti-human (raised in a goat host,
recognizing a human protein) then the secondary antibody needs to be specific to
goat IgG. A common example would be a donkey anti-goat IgG (raised in a donkey
host to detect goat IgG).

Detection of Mouse CD31/PECAM‑1 by Western Blot. Western blot shows lysates of
bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of
Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF017). A specific band was detected for CD31/PECAM-1 at
approximately 130 kDa (as indicated). This experiment was conducted under
reducing conditions and using our Western blot Buffer Group 1.

Detection of ACE-2 in Human PBMC by Flow Cytometry. Human PBMC were stained with
(A) Rat Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB9334) or (B) Rat
IgG2b Isotype Control Antibody (MAB0061) followed by Allophycocyanin-conjugated
Anti-Rat IgG Secondary Antibody (F0113) and Mouse anti-Human CD14
Phycoerythrin-conjugated Monoclonal Antibody (FAB3832P). Staining was performed
using our Staining Membrane-associated Proteins protocol.



 



VISUCYTE™ HRP POLYMER



VisUCyte™ HRP Polymer Antibodies are a biotin-free detection reagent that
removes the need to perform additional quenching of endogenous biotin and avidin
present in some tissues. By removing the need for need for additional blocking
and incubation steps, researchers can achieve specific staining in cells and
tissue much faster than the conventional avidin-biotin-HRP procedure. VisUCyte
HRP polymer also provides higher sensitivity allowing researchers to use 2-3
times less primary antibody when compared to the conventional avidin-biotin-HRP
protocols.

Learn About VisUCyte HRP Polymer

For added convenience, we also offer Cell and Tissue Staining Kits that include
secondary antibodies, blocking reagents, streptavidin HRP, and a chromogen
reagent.

Browse Cell and Tissue Staining Kits





 



NORTHERNLIGHTS™ SECONDARY ANTIBODIES AND STREPTAVIDIN CONJUGATES



NorthernLights™ fluorescent secondary antibodies and streptavidin conjugates are
bright and resistant to photobleaching. These conjugates are great options that
work well in Flow cytometry and fluorescence microscopy. Their spectral
properties are similar to several widely used fluorochromes and are suitable for
use with common filter sets and lasers. These secondary antibodies are available
with three distinct excitation and emission wavelengths, making them an optimal
option for multi-color experiments. NorthernLights secondary antibodies are
stable when exposed to alcohols and xylene, as well as DPX mounting medium
making these fluorescent conjugates ideal for fluorescent microscopy.

View all NorthernLight Products

Rat cortical stem cell differentiation was monitored using multi-color
immunocytochemistry. Neural progenitors were labeled with Goat anti-Rat Nestin
Polyclonal Antibody (Catalog # AF2736) and stained with Donkey anti-Goat
NorthernLights-493 Secondary Antibody (Catalog # NL003; green). Differentiated
neurons were labeled with Mouse Anti-Neuron-specific beta -III Tubulin
Monoclonal Antibody (Catalog # MAB1195) and stained using Donkey anti-Mouse
NorthernLights-557 Secondary Antibody (Catalog # NL007; red). Nuclei were
stained with DAPI (blue).



 



ADDITIONAL ANTIBODY RESOURCES



Primary Antibodies

Conjugated Antibodies

Cell and Tissue Staining Kits

Isotype Controls

VisUCyte HRP Polymer

NorthernLights Secondary Reagents

 



FEATURED CONTENT


FEATURED CONTENT

VISUCYTE™ HRP POLYMER





Get cleaner and faster results in Immunohistochemistry staining with VisUCyte
HRP Polymer.



Learn About VisUCyte


FEATURED CONTENT

EPITOPE TAG ANTIBODIES





Detect your molecule of interest with our highly specific epitope tag
antibodies.



View Our Epitope Tag Antibodies


FEATURED CONTENT

ISOTYPE CONTROLS





Discover your next isotype control for your primary antibody. We offer options
across multiple species, isoforms, and conjugates



Find Your Isotype Control
Need help? ×

How can we help you?

Close Start Chat



RELATED INFORMATION

Antibodies
Immunohistochemistry Troubleshooting Guide


POPULAR PRODUCTS

 * Bioactive Proteins
 * Luminex Assays
 * ELISA Kits
 * Small Molecules
 * Primary Antibodies


COMPANY INFORMATION

 * About Us
 * Careers
 * R&D Systems Quality
 * Bio-Techne Brands


SUPPORT

 * Contact Us
 * Ordering R&D Systems Products
 * Distributors
 * Protocols
 * FAQs


STAY CONNECTED

Get the latest news, product updates, and promotions:

Subscribe

Follow R&D Systems:



Legal | Terms & Conditions | Privacy and Cookie Policy | Sitemap
© Copyright 2024 R&D Systems, Inc. All Rights Reserved.






‹›x›








‹›x›










Live chat:Chat now