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* Human RefSeq Panel
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* Mouse Exome Panel
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* Combinatorial Variant Library
* Spread Out Low Diversity Libraries
* Site Saturation Libraries
* Enzyme Screening KitsNew
Synthetic Controls
* Liquid Biopsy
* cfDNA Pan-Cancer Reference Standards v2New
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* Respiratory Virus Controls
* SARS-CoV-2 Controls
* Mpox Virus Controls
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* Critical Reagents and Tool Antibodies
* Antigen Production
* In Vitro >
* Antibody Discovery
* Antibody Optimization
* Antibody Optimization and Engineering
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* Diversimab Platform
* VHH Discovery
* Antibody Discovery
* Antibody Sequencing
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* CHO Express Antibodies
* HEK293 Express Antibodies
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* Antibody Screening
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* cfDNA Pan-Cancer Reference Standards v2New
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* SARS-CoV-2 Research Panel
* SARS-CoV-2 RNA Controls
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* Anti-ACE2
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OLIGO POOLS
Exceptional performance at any Scale
Exceptional performance at any Scale
Exceptional performance at any Scale
ORDER NOW
OVERVIEW OLIGO POOLS NEW CLONED OLIGO POOLS APPLICATIONS RESOURCES
OVERVIEW OLIGO POOLS NEW CLONED OLIGO POOLS APPLICATIONS RESOURCES
ORDER NOW
Overview
PERFORMANCE AT ANY SCALE
Our revolutionary silicon-based DNA Synthesis platform is capable of producing
over a million unique ssDNA oligos in a single run. Each chip contains Thousands
of discrete clusters each containing 121 individually addressable surfaces
capable of synthesizing a unique oligo sequence. A representation of the silicon
chip technology can be seen below.
Due to the sheer scale of DNA synthesis capability on the chip there are
virtually no limitations to the number of unique oligos that can be assembled
into a pool. Don't restrict the outcome of your application, order Oligo Pools
tailored precisely to your experiment.
MORE SPACE FOR INNOVATION
Don’t be limited by length. Encode the elements and sequences you need,
including promoters, enhancers, guides, and probes all within the expanded
architecture. With oligo lengths up to 300nt it’s possible to encode multiple
elements in a single design giving you the ability to do more with each oligo.
SCREEN ONCE, SCREEN RIGHT
Unmatched pool uniformity without bias provides more unique targets per screen.
This means less effort is required to achieve the data coverage you need, saving
time and money.
BE DATA CONFIDENT
With the precision and uniformity of Twist Oligo Pools, you can be confident in
the quality of your experimental results. Don’t miss a datapoint due to
synthesis errors or inefficiencies. Get industry-leading oligo representation
and low error rate for oligos of any length up to 300nt, and pools of any size.
Leverage these exceptional quality oligo pools at the precise scale and length
required for your application.
HAVE QUESTIONS?
Tell us what you would like more information on. We’re here to help!
Contact Us
WHY DOES UNIFORMITY MATTER?
LEARN MORE
OLIGO POOLS
OLIGO POOLS
Twist Oligo Pools are highly diverse collections of single-stranded
oligonucleotides synthesized using our silicon-based DNA writing technology. Our
synthesis platform enables massively parallel production of hundreds of
thousands of high-quality, accurate oligos per run, allowing the generation of
complex and diverse oligo pools for use in applications like CRISPR screening.
HOW DOES IT WORK?
You provide us with your oligo sequences and we will synthesize your user
designed oligo pool libraries, enabling you to spend your time working on
experimentation and discovery.
HIGHLY UNIFORM OLIGO SYNTHESIS
NGS quality control data from a typical oligo pool containing 23,000 90mer
oligos show the uniformity of the pool at 300x read coverage. The corresponding
table indicates the uniformity metrics for this pool. Twist oligos are
synthesized bias-free with high uniformity and complete oligo representation.
SEQUENCING ANALYSIS OF OLIGO POOLS
Sequencing analysis of Oligo Pools generated by Twist Bioscience and an
array-based competitor demonstrate that the Twist Oligo Pools contain 100% of
the expected sequences and a higher percentage of correct sequences than the
competitor pool.
--------------------------------------------------------------------------------
SPECIFICATIONS
Oligo Length Up to 300nt Oligo Pool Size No limits on pool size Yield >0.2 fmol
average of each oligo Uniformity >90% oligos represented within <2.0x of the
mean Error rate Up to 1:3000 Turn-Around Time As few as 3 days* Price
Industry-leading pricing
*Turnaround time for the standard Oligo Pool offering varies based on length and
sequence complexity. Oligo Pools at 20-121 nucleotides in length range from 3-6
business days, 121-200 nucleotides in length range from 3-8 business days, and
201-300 nucleotides in length range from 5-10 business days.
HAVE QUESTIONS?
Tell us what you would like more information on. We’re here to help!
Contact Us
New Cloned Oligo Pools
NEW CLONED OLIGO POOLS
Oligo pool quality is the foundation of a successful experiment. Errors during
synthesis or cloning can easily skew oligo pool quality, leading to over and
underrepresentation of desired sequences. Twist now offers an optimized cloning
service that allows you to further avoid the hardship that comes with testing
PCR amplification conditions, selecting the right polymerases and primer pairs,
as well as designing a cloning workflow. Skip the drama and let Twist Bioscience
synthesize and clone oligo pools for you.
HOW DOES IT WORK?
Just two steps away from your custom cloned Oligo Pools. You provide us with
your oligo sequences and we will synthesize, amplify, and clone your user
designed oligo pool libraries, enabling you to spend your time working on
experimentation and discovery.
TWIST BIOSCIENCE’S CLONED OLIGO POOLS OF ALL LENGTHS HAVE HIGH UNIFORMITIES AND
LOW ERROR RATES
PERFORMANCE DATA OF AMPLIFIED AND CLONED OLIGO POOLS UNDER 150 NUCLEOTIDES IN
LENGTH EVEN WITH HIGH GC CONTENT:
--------------------------------------------------------------------------------
PERFORMANCE DATA OF AMPLIFIED AND CLONED OLIGO POOLS UP TO 300 NUCLEOTIDES IN
LENGTH EVEN WITH HIGH GC CONTENT:
--------------------------------------------------------------------------------
ACHIEVE THE HIGHEST QUALITY AND ACCURACY IN YOUR EXPERIMENTAL DATA
Twist’s cloned oligo pools not only have low error rates and high uniformity,
but also have low chimera rates. Chimeras are unwanted hybrid molecules that are
created as a result of suboptimal PCR conditions which leads to improper
amplification and recombination of different oligos within the oligo pool. See
our illustration below for an example of an on-target cloned pool versus an
off-target one that contains chimeras, which ultimately leads cloned oligo pools
that are comprised of undesired sequences:
With Twist, you never have to worry about Chimeras! See for yourself:
Traditional Amplification vs Twist In-House Amplification Method Performance
SPECIFICATIONS
Oligo Length Up to 300nt Oligo Pool Size No limits on pool size Yield >0.2
fmol average of each oligo/td> Uniformity >90% oligos represented within <5.0x
of the mean Chimera rate As low as 1.5%* Turn-Around Time As few as 4 weeks*
*Terms and Conditions: Baseline turnaround time for Cloned Oligo Pools, which
includes cloning and amplification, of up to 100 and 300 nucleotides is 4-6
weeks and 6-8 weeks, respectively. An additional 2 weeks will be added to this
baseline turnaround time for all Cloned Oligo Pools, regardless of length, that
require new vector onboarding. Chimera rate, drop out rate, and uniformity will
vary based on sequence complexity.
HAVE QUESTIONS?
Tell us what you would like more information on. We’re here to help!
Contact Us
Applications
GUIDE RNA LIBRARIES FOR CRISPR SCREENS
CRISPR screens involve the precise, high-throughput knock-out, interference or
modulation of gene activity, allowing complex genotype-phenotype interactions to
be unveiled. Oligo Pools are designed to encode multiple guide RNA molecules per
gene.
Product benefits include:
* Full control over guide library design, avoiding unwanted guides, wasted
sequencing, and limited guide-per-gene ratios.
* Pairing high-fidelity Cas enzymes with highly accurate synthesis for true
high-fidelity screens with minimal off-target effects.
* Incredibly uniform, bias-free pools achieve amplified signal to noise ratio
to achieve maximum hit detection, lowering downstream costs.
* Oligos up to 300nt make space for additional experimental elements. Add
multiple guides per oligo, genome homology, or minimal promoters with ease.
Watch our webinar: Scalable and combinatorial single-cell CRISPR screens by
direct guide RNA capture and targeted seq
GENOME TILING LIBRARIES
Tiling libraries allow a comprehensive study of genomic functionality. Pools of
oligos may encode peptide arrays, mRNA encoding sequences, or genome fragments.
Product benefits include:
* Tiling precise genomic regions of interest. Reduce experimental noise by
ignoring unwanted sequences at the design step.
* Complex, repetitive or palindromic sequences that cannot typically be
synthesized as genes can be encoded in oligos.
* Larger peptide structures or genome architectures can be encoded in oligos
300nt in length.
* Uniform synthesis minimizes oversampling required for comprehensive
screening.
Read our article: Identifying Coronavirus Virulence Factors for RNA Vaccine
Development with Twist Oligo Pools
HIGH THROUGHPUT GENETIC CONTROL SCREENS
Understanding the genetic elements controlling gene expression and regulation
has broad application in synthetic biology and development of potential disease
treatment. Oligo Pools power high-throughput screens of genetic control,
including gene expression profiling and reporter-based applications.
Product benefits include:
* High-quality pools ensure you can interrogate every base for functionality
* High uniformity means you screen less to find your answers
* Up to 300nt pools gives you the flexibility to add multiple elements or
homology regions
Watch our webinar: Epitranscriptome Screening on TechNetworks
FLUORESCENCE IN-SITU HYBRIDIZATION
Fluorescence in-situ hybridization (FISH) allows the abundance and spatial
organization of nucleic acids in biological samples to be directly visualized.
Oligo Pools can encode probes that when bound to fluorescent markers provide
tunable, programmable visualization of large numbers of targets.
Product benefits include:
* Rapid FISH probe development
* Sequence control enables effective targeting
* Longer oligos enable more specific targeting
Watch our webinar: Rapid and efficient production of FISH probes using oligo
pools with Nicola Crosetto
Resources
Product Sheet
READ
PRODUCT SHEET
Twist Oligo Pools
READ
PRODUCT SHEET
Twist Cloned Oligo Pools
Protocol
READ
PROTOCOL
Oligo Pools Amplification Guidelines
READ
PROTOCOL
Twist DNA Resuspension Guidelines
These guidelines outline the best practices for resuspending Twist Gene
Fragments, Clonal Genes, Oligo Pools, and Variant Libraries.
Case Study
READ
CASE STUDY
Designing Proteins to Revolutionize Biotechnology
Webinar
WATCH
WEBINAR
Next generation CRISPR screens based on single-cell transcriptomics
WATCH
WEBINAR
Using amino acid composition and conservation for more efficient CRISPR screens
WATCH
WEBINAR
Rapid and efficient production of FISH probes using oligo pools
WATCH
WEBINAR
Epitranscriptome Screening
WATCH
WEBINAR
CRISPR CAS9 Screening in Human Pluripotent Stem Cells
WATCH
WEBINAR
Discovering PARP inhibitor resistance mechanisms using genome-wide and focused
CRISPR screens
WHAT SCIENTISTS HAVE TO SAY
“
Twist’s ssDNA oligo pools are well suited for making pooled RNA structures
because of their high uniformity, low error rate, and lengths up to 300 nt.
These features provide the screening of RNA-small molecule interactions with
high accuracy and efficiency.
Kaoru R. Komatsu
Representative Director and Chief Technology Officer • xFOREST Therapeutics Co.,
Ltd
“
We have found Twist Oligo Pools to be a fast, convenient and flexible route to
synthesizing high quality customized CRISPR libraries for our research.
Stephen Pettit
Staff Scientist • Institute of Cancer Research, UK
“
We work on massively parallel reporter assays (MPRAs) focused on identifying
gene regulatory functions of single base substitutions, so high oligo fidelity
is critical. When we sequenced the pool that we got from Twist the quality was
astounding, with twofold fewer mutations than we were expecting.
Bernie Mulvey
MD-PhD Student • Washington University School of Medicine • St. Louis, MO, USA
FINALLY, AN EASY WAY TO ORDER DNA
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