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Porous Organic Frameworks MOF Ligands COF Ligands
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Materials Battery Materials Cathode Active Materials Anode Active Materials
Optoelectronic Materials Photonic & Optical Materials Liquid Crystals
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Fullerene Acceptors Non-Fullerene Acceptors
Semiconducting Polymers OPV Polymers OFET and OLED Polymers Interface
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Precursors Perovskite Interface Materials Perovskite Quantum Dots
Self-Assembled Monolayers
DSSC Dyes and Electrolytes
DSSC Dyes & Electrolytes
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PEDOT:PSS and PEDOT Polymer Blends Small Molecule OPV Donors Cathode
Interlayer Materials Green Energy Materials Popular and New Materials
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SYRINGE PUMP USER MANUAL
Syringe Pump (single) User Manual (PDF)
Syringe Pump (dual) User Manual (PDF)
CONTENTS
--------------------------------------------------------------------------------
1. Overview
2. EU Declaration of Conformity (DoC)
3. Safety
1. Warning
2. Use of Equipment
3. Hazard Icons
4. General Hazards
5. Power Cord Safety
6. Servicing
7. Health and Safety - Operation
8. Health and Safety - Servicing
4. Equipment Purpose
5. Unpacking
1. Packing List
2. Damage Inspection
6. Specifications
7. System Components
8. Installation
9. Operation
1. Syringe Pump Schematic
2. Attaching a Syringe to the System
3. User Interface
4. Program Operation
1. MODEL: L2003S1 (Single Syringe Pump)
2. MODEL: L2003D1 (Dual Syringe Pump)
10. Maintenance
11. Troubleshooting
1. OVERVIEW
--------------------------------------------------------------------------------
Syringe pumps are used in the field of scientific research to provide a reliable
and repeatable method for moving liquids. Typically, the most common use of a
syringe pump is to dispense a liquid at a known rate. This can be for use in
processes such as pre-metered coating, automated systems, chemical synthesis,
microfluidics, and flow chemistry. Other uses of syringe pumps include the
withdrawal of liquids from a system for processes such as automated sample
taking, and fluid level adjustment. By combining both infusion and withdrawal
from syringes it is possible to use a syringe pump for an even wider array of
applications including continuous flow of solutions, solution mixing, and the
emulsification of solutions
A syringe pump works by fixing the barrel of the syringe to the body of the
equipment while at the same time fixing the plunger to a moving platform. This
movement is achieved via the use of a high accuracy stepper motor where the
distance moved by the platform is well known and characterised. By knowing the
internal diameter of the syringe being used it is possible to work out the
volume displaced when the platform moves. The Ossila Syringe Pump provides you
with a simple easily programmable system, where each program can have up to 99
different steps in which the rate, volume, and/or time of infusion/withdrawal
can be chosen. By selecting the correct diameter syringe, it is possible to
achieve flow rates as low as nanolitres per minute or as high as millilitres per
second.
1.1 APPLICATIONS
* Solution dispensing
* Solution mixing
* Emulsification
* Metered coatings
* Solution quenching
* Electrospinning
* Electrospraying
* Microfluidics
* Flow chemistry
2. EU DECLARATION OF CONFORMITY (DOC)
--------------------------------------------------------------------------------
We
Company Name: Ossila BV
Postal Address: Biopartner 3 Building, Galileiweg 8
Postcode: 2333 BD Leiden
Country: The Netherlands
Telephone Number: +31 (0)718 081020
Email Address: info@ossila.com
declare that the DoC is issued under our sole responsibility and belongs to the
following product:
Product: Single Syringe Pump (L2003S1), Dual Syringe Pump (L2003D1)
Serial Number: L2003S1 - xxxx, L2003D1
Object of Declaration
Single Syringe Pump (L2003S1), Dual Syringe Pump (L2003D1)
The object of declaration described above is in conformity with the relevant
Union harmonisation legislation:
Machinery Directive 2006/42/EC
EMC Directive 2014/30/EU
RoHS Directive 2011/65/EU
The folowing harmonised standards and technical specifications have been
applied:
BS EN ISO 12100:2010 Safety of machinery - General principles for design - Risk
assessment and risk reduction
Signed:
Name: Dr James Kingsley
Place: Leiden
Date: 16/11/2021
Декларация за съответствие на ЕС Производител Ossila BV, Biopartner 3 building,
Galileiweg 8, 2333 BD Leiden, NL. Декларира с цялата си отговорност, че
посоченото оборудване съответства на приложимото законодателство на ЕС за
хармонизиране, посочено на предходната(-ите) страница(-и) на настоящия документ.
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na předchozích stranách tohoto dokumentu.
[Dansk] EU-overensstemme lseserklærin g Producent Ossila BV, Biopartner 3
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Galileiweg 8, 2333 BD Leiden, NL. Wir erklären in alleiniger Verantwortung, dass
das aufgeführte Gerät konform mit der relevanten EUHarmonisierungsgesetzgebung
auf den vorangegangenen Seiten dieses Dokuments ist.
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som förtecknas överensstämmer med relevanta förordningar gällande
EUharmonisering som fmns på föregående
3. SAFETY
--------------------------------------------------------------------------------
3.1 WARNING
Pinch points present when in operation, keep hands clear of moving stage.
3.2 USE OF EQUIPMENT
CAUTION: For research use only. Not for clinical use on patients.
The Ossila Syringe Pump is designed to be used as instructed, it is intended to
be used under the following conditions:
* Indoors in a laboratory environment (pollution degree 2).
* Altitudes up to 2000 m.
* Temperatures of 5°C to 40°C; maximum relative humidity of 80% up to 31°C.
The Syringe Pump is supplied with a 24V/2A power adapter with a power cord for
the country of purchase, in accordance with European Commission regulations and
British Standards. Use of any other electrical power cables, adaptors or
transformers is not recommended.
3.3 HAZARD ICONS
The symbols shown in Table 3.1 can be found at points throughout the manual.
Note each warning before attempting any associated operations.
Table 3.1 Hazard warning labels used in this manual.
Symbol Associated Hazard General warning or caution, which accompanying text
will explain Electrical shock Pinch point or entanglement hazard
3.4 GENERAL HAZARDS
Before installing or operating the Syringe Pump, there are several health and
safety precautions which must be followed and executed to ensure safe
installation and operation.
WARNING: Improper handling when operating or servicing this equipment can result
in serious injury. Read this manual before operating or servicing this
equipment.
CAUTION: During operation, the movement of the plunger clamp and the rotation of
the lead screw/coupler could result in a pinch point hazard and/or an
entanglement hazard. Caution should be taken whenever the system is in
operation.
3.5 POWER CORD SAFETY
Emergency power disconnect options: Use the power cord as a disconnecting method
and remove it from the power source. To facilitate disconnect, make sure the
power outlet for this cord is readily accessible to the operator.
3.6 SERVICING
If servicing is required, please return the unit to Ossila Ltd The warranty will
be invalidated if:
* Modification or service has taken place by anyone other than an Ossila
engineer.
* The unit has been subjected to chemical damage through improper use.
* The unit has been operated outside the usage parameters stated in the user
documentation associated with the unit.
* The unit has been rendered inoperable through accident, misuse,
contamination, improper maintenance, modification, or other external causes.
3.7 HEALTH AND SAFETY - OPERATION
Pinch point and entanglement hazards are present during operation of the syringe
pump. As some precaution users should avoid handling or leaning over the
equipment during operation in order to avoid possible crushing or entanglement
of hair and/or clothing.
3.8 HEALTH AND SAFETY - SERVICING
Service or installation work that includes integrating electrical components
should be performed by an Ossila engineer only. Never alter the wiring of any
purchased equipment. If changes are made, such alterations may damage the
equipment, cause injury or death. At the very least, such alterations may void
your equipment’s warranty.
4. EQUIPMENT PURPOSE
--------------------------------------------------------------------------------
The Ossila Syringe Pump has been designed to provide users with a tool to move
volumes of fluids accurately and repeatedly at specified rates. The equipment
can be used in a wide variety of experiments where accurate dispense volumes
and/or dispense rates are required. In order to achieve this, the system uses a
stepper motor to enable movement of specified distances. This is done by
securing the body of the syringe to the frame of the syringe pump while at the
same time securing the syringe plunger to the moving holder. The internal
diameter of the syringe can be used to determine the volume displaced by the
movement of the stepper motor and plunger. By choosing the appropriate syringe
size, a wide range of dispense rates can be achieved.
The exact syringe types, deposition rates, deposition volumes, and solutions
used are up to the user to choose and will vary depending upon the type of
experiment being undertaken. For more information on uses please see the
operating instructions and guidelines, for more information on the dispensing
limits please see the specifications.
5. UNPACKING
--------------------------------------------------------------------------------
5.1 PACKING LIST
The standard items included with the Syringe Pump are:
* The Syringe Pump unit
* Universal power adapter
5.2 DAMAGE INSPECTION
Examine the components for evidence of shipping damage, if damage has occurred,
please contact Ossila directly for further action. The shipping packaging will
come with a shock indicator to show if there has been any mishandling of the
package during transportation
6. SPECIFICATIONS
--------------------------------------------------------------------------------
The specifications for the syringe pump software are shown in Table 6.1, and the
hardware specifications are shown in Table 6.2.
Table 6.1. Ossila Syringe Pump software specifications
Number of Programmes 10 Steps per Programme 99 Syringe Control Independent
Control Syringe Size Settings 5 pre-sets, 1 customisable diameter Force Tuning
5% to 100% (5% increments) Minimum Syringe Size 0.5μl Memory Non-Volatile Memory
Table 6.2. Ossila Syringe Pump hardware specifications
Syringes 1 (L2003S1); 2(L2003D1) Operation mode Infusion and withdrawal Rate
accuracy ±3% Drive motor 1.8° Stepper motor Max linear force 500N at 100% force
Motor drive controller Microprocessor with 1/64 microstepping Number of
microsteps per revolution 12,800 Screw pitch 1mm Distance per microstep 78nm
Maximum step rate/distance 27 μstep.min-1; 5mm.s-1 Minimum step rate/distance
27sec.μstep-1; 12μm.s-1 Maximum syringe size 50 (60)ml Safety Limit switches;
crash sensing Power 24 V DC; 2.0 A Power supply Input voltage range 100 -
240VAC; 50/60Hz; 50VA Operating temperature/humidity 5°C to 40°C; Up to 80% RH @
31°C Overall dimensions
Width: 330mm
Height: 140mm
Depth: 260mm
7. SYSTEM COMPONENTS
--------------------------------------------------------------------------------
The Syringe Pump (L2003S1/L2003D1) comprises two items:
* Syringe Pump unit (Figure 7.1)
* 24 V mains power supply adapter (Figure 7.2)
Figure 7.1 Syringe Pump Figure 7.2 24V mains power supply adapter
The Syringe Pump unit is powered from a 24 V, 2 A mains power supply adapter.
This low voltage supply is convenient and reduces the health and safety risks
involved.
8. INSTALLATION
--------------------------------------------------------------------------------
1. Place the unit on a solid, level work surface.
I. Ensure the area is free from vibrations, temperature extremes and highly
flammable or explosive materials.
2. Before plugging in the Syringe Pump, ensure the power switch on the unit is
switched to the ‘0’ position (off).
3. Connect the power supply first to the mains outlet and second to the Syringe
Pump unit.
I. See Figure 8.1 for connecting the Syringe Pump to the power supply
cable.
4. Set the Syringe Pump power switch to the ‘1’ position to turn on.
Figure 8.1 Installation of th Syringe Pump by plugging in the power supply cable
9. OPERATION
--------------------------------------------------------------------------------
9.1 SYRINGE PUMP SCHEMATIC
A top-down view of the Syringe Pump is shown in Figure 9.1 with all the relevant
components highlighted.
Figure 9.1 Syringe Pump schematic
9.2 ATTACHING A SYRINGE TO THE SYSTEM
When using the Syringe Pump the following procedures should be done while
attaching the syringe to the system to achieve the best results.
1. The solution should be preloaded into the syringe; make sure all air bubbles
are removed from the syringe before attaching to the system.
2. The syringe barrel should be placed flat into the groove on the syringe
holder, and the barrel top should be placed inside the clamping mechanism
(see Figure. 9.2). The top clamping arm should be lowered onto the top of
the syringe and locked into place by tightening the wing nut on the side of
the syringe holder. The barrel top should be clamped into place by
tightening the wing nut screws as the opposite end of the syringe holder.
Figure 9.2 Placement of the syringe into the groove along with the barrel top
placed in the clamping mechanism Figure 9.3 Placement of the top clamping arm
Figure 9.4 Fixing of the barrel top into clamping mechanism
3. Enter manual mode on the syringe and drive the plunger holder till it rests
against the end of the plunger. Slide the end of the plunger into the
clamping mechanism and tighten, making sure it is parallel with the barrel
of the syringe.
Figure 9.5 Plunger clamping mechanism
4. Connect any additional tubing or fixtures required for your experiment.
Using the manual mode preload the fixtures and tubing with your solution.
9.3 USER INTERFACE
Figure 9.6 Syringe Pump LCD screen and keypad
Table 9.1. Operational buttons and their associated functions
Button Function S (Settings) Enters the Settings menu where syringe diameter,
and maximum force can be set M (Manual) Enters Manual mode where the plunger
holder can be moved P (Program) Enters the Program mode where saved programs can
be selected and/or edited Navigates ‘Up’ through menus; increases selected
values by 1; increases unit size Navigates ‘Down’ through menus; decreases
selected values by 1; decreases unit size Navigates ‘Right’ through menus
Navigates ‘Left’ through menus Press to select, edit, or accept changes Stop
current running program Run currently selected program
9.4 PROGRAM OPERATION
9.4.1 MODEL: L2003S1 (SINGLE SYRINGE PUMP)
WARNING! Pinch points and entanglement hazards!
The rotation of the lead screw/coupler and the movement of the plunger holder
present entanglement and pinch hazards. Care should be taken when the system is
running.
1. Turn the Ossila Syringe Pump power switch on (position ‘1’); the bootup
screen will show as below.
2. The system automatically goes to Settings mode screen after the bootup
screen. To set the syringe diameter enter the settings menu by pressing the
“S” Button, navigate to syringe selection (as shown in the image below).
Press “OK” to select what diameter of syringe to choose.
I. For syringes purchased from Ossila there are several pre-set diameters for
quick selection, these are 1ml, 2ml, 5ml, 10ml, and 50ml. To change the
syringe size setting, Press “OK” and navigate the syringe number by pressing
“Left” or “Right” buttons.
II. For custom diameters navigate to custom and press “OK” to edit. To edit the
diameter, highlight diameter and press “OK”. Use “Up” or “Down” and “Left”
or “Right” buttons to set the internal diameter and press “OK” to set the
value. Highlight the volume and press “OK” to edit.
A warning will appear if the diameter is not set for a custom syringe.
A warning will appear if the volume is not set for a custom syringe.
III. To change the force setting value for the syringe, navigate to force and
press “OK” to edit. To change the value, press “Up” or “Down” buttons for
increment/decrement value of 5.
3. To move the plunger holder without setting a program press the “M” button,
this will take you to Manual mode (as shown in the image below). Press
“Left” or “Right” buttons to move the plunger holder towards the coupler or
towards the syringe clamp. Press “OK” to cycle through pre-set speeds.
4. To set a program to run automatically go to the program menu by pressing the
“P” button, select the program you wish to edit and run by navigating to the
program number (as shown in the image below) and pressing “OK”. Pressing
“Up” or “Down” buttons will allow you to switch between pre-saved programs.
I. To change the program number, press the “Up” button until the program number
highlighted and press “Left” or “Right” buttons to change the program
number.
II. To edit the number of steps, navigate to the steps line and press “OK” to
edit and “Up” or “Down” buttons to increase or decrease the value. When
finished press “OK” again to save the changes.
III. To change the step number, press “Left” or “Right” buttons to cycle
through the different steps.
IV. To edit the dispense rate navigate to the dispense rate line and press “OK”
to begin editing. Use “Left” or “Right” buttons to highlight the number you
wish to edit and “Up” or “Down” buttons to increase or decrease the value.
When finished press “OK” again to save the changes.
Note: The dispense time for the step will automatically update to account for
the new rate.
V. Navigate to the unit to change the dispense rate unit by pressing “Up” or
“Down” buttons to change from microliter (μl) to millilitre (ml) or vice
versa.
A warning will appear if the set rate is higher than allowed for the syringe
size. The value will be recalculated and set to max rate for the particular
syringe.
VI. To edit the dispense volume navigate to the dispense volume line and press
“OK” to begin editing. Use “Left” or “Right” buttons to highlight the
number you wish to edit and “Up” or “Down” buttons to increase or decrease
the value. When finished press “OK” again to save the changes.
Note: The dispense time for the step will automatically update to account for
the new rate.
VII. Navigate to the unit to change the dispense volume unit by pressing “Up”
or “Down” buttons to change from microliter (μl) to millilitre (ml) or
vice versa.
A warning will appear if the set volume higher than allow for the syringe size.
The value will be recalculated and set to max volume for the syringe.
VIII. The user can either dispense or infuse the solution using Ossila Syringe
Pump by navigating to volume sign (+), and press “Up” or “Down” buttons
to change the sign. The ‘+’ sign indicates dispensing and ‘–’ indicates
infusing.
IX. The user can edit the time by navigating to dispense time and pressing “OK”
to edit. The format of time is HH:MM:SS (H – Hour, M – Minute, S – Second).
The dispense volume for the step will automatically update to account for the
new dispense time setting.
A warning will appear if the volume is larger than allowed for the syringe size.
The value will be recalculated to the correct volume for the syringe.
5. The user can run the program by pressing the “Start” button. The running
program page will then appear to indicate this.
I. Once the dispensing is complete, a message with pop up to notify the user.
II. The user can stop the operation by pressing the “Stop” button and the
warning will appear as follows.
The Ossila Syringe Pump is equipped with a safety warning.
The System temperature is monitored while the system is On. In the case of high
temperatures, a warning will appear to notify the user.
9.4.2 MODEL: L2003D1 (DUAL SYRINGE PUMP)
WARNING! Pinch points and entanglement hazards!
The rotation of the lead screw/coupler and the movement of the plunger holder
present entanglement and pinch hazards. Care should be taken when the system is
running.
1. Turn the Ossila Syringe Pump power switch on (position ‘1’); the bootup
screen will show as below.
2. To set the syringe diameter enter the Settings menu by pressing the “S”
button, navigate to syringe selection (as shown in the image below). Press
“OK” to select what diameter of syringe to choose.
I. For syringes purchased from Ossila there are several pre-set diameters for
quick selection. These include 1ml, 2ml, 5ml, 10ml, and 50ml.
II. For custom diameters navigate to custom and press “OK” to edit. To edit the
diameter, highlight diameter and press “OK” use “Up” or “Down” and “Left”
or “Right” buttons to set the internal diameter and press “OK” to set the
value. Highlight the volume and press “OK” to edit.
A warning will appear if the diameter is not set for custom syringe.
A warning will appear if the volume is not set for custom syringe.
III. To change the force setting value for the syringe, navigate to force and
press “OK” to edit. The change the value, press “Up” or “Down” buttons for
increment/decrement value of 5.
3. To move the plunger holder without setting a program press the “M” button.
This will take you to Manual mode (as shown in the image below). Press “Up”
or “Down” buttons to select the syringe you want to move. Press “Left” or
“Right” buttons to move the plunger holder towards the coupler or towards
the syringe clamp. Press “OK” to cycle through pre-set speeds for selected
syringe.
4. To set a Program to run automatically go to the program menu by pressing the
“P” button. Select the program you wish to edit and run by navigating to the
program number (as shown in the image below) and press “OK”. Pressing “Up”
or “Down” buttons will allow you to switch between pre-saved programs.
I. The program can be edited by changing the number of steps, the dispense rate
for each step, and the dispense volume for each step.
II. To edit the number of steps, navigate to the steps line and press “OK” to
edit use “Left” or “Right” buttons to highlight the number you wish to edit
and “Up” or “Down” buttons to increase or decrease the value. When finished
press “OK” again to save the changes.
III. To change the step number you are on, navigate to the steps line and press
“Left” or “Right” buttons to cycle through the different steps.
IV. To edit the dispense rate navigate to the dispense rate line and press “OK”
to begin editing. Use “Left” or “Right” buttons to highlight the number you
wish to edit and “Up” or “Down” buttons to increase or decrease the value.
When finished press “OK” again to save the changes
The dispense time for the step will automatically update to account for the new
rate.
V. Edit the dispense rate by pressing “Up” or “Down” buttons to change the
units from microliter (μl) to millilitre (ml) or vice versa.
A warning will appear if the set rate higher than allow for the syringe size.
The value will be recalculated and set to max rate for the particular syringe.
VI. To adjust the dispense volume navigate to the dispense volume line and
press “OK” to begin editing. Use “Left” or “Right” buttons to highlight the
number you wish to edit and “Up” or “Down” buttons to increase or decrease
the value. When finished press “OK” again to save the changes.
The dispense time for the step will automatically update to account for the new
rate.
VII. Change the dispense volume unit by pressing “Up” or “Down” buttons to
switch from microliter (μl) to millilitre (ml) or vice versa.
A warning will appear if the set volume is larger than the syringe size can
allow. The value will be recalculated and set to the max volume for the syringe.
VIII. The user can either dispense or infuse the solution using Ossila Syringe
Pump by navigating to volume sign (+), and press “Up” or “Down” buttons
to change the sign. The ‘+’ sign indicates dispensing and ‘–‘ indicates
infusing.
IX. The user can edit the time by navigating to the dispense time and pressing
“OK” to edit. The format of time is HH:MM:SS (H – Hour, M – Minute, S –
Second).
The dispense volume for the step will automatically update to account for the
new dispense time setting.
A warning will appear if the volume is larger than the syringe size allows. The
value will be recalculated to the correct volume for the syringe.
X. To edit the parameter for syringe 2 (S2), navigate the cursor down by
pressing “Up” or “Down” buttons and press “OK” to edit an individual
parameter value.
5. The user can run the program by pressing the “Start” button. The running
program page will appear to indicate the program is running.
I. Both syringes are dispensing
II. Syringe 1 is dispensing and syringe 2 is infusing
III. Syringe 1 is infusing and syringe 2 is dispensing
IV. Both syringes are infusing
Once the timer is finished, a warning will appear to alert the suer that
dispensing is complete.
If the one of the syringes completes dispensing, the “complete” message will pop
up to notify the user.
While the system is running, the user can stop one of the syringes by pressing
the “Up” or “Down” buttons. Press “OK” to stop the selected syringe.
If both syringes are stopped by the user, the message will appear as follow.
To stop both syringes simultaneously, press the “Stop” button. The message will
appear as follow.
The Ossila Syringe Pump is equipped with a safety warning.
The System temperature is monitored while the system is On. In the case of high
temperatures, a warning will appear to notify the user.
The system is also equipped with safety switches in case of software
malfunction. There are two switches to safeguard forward and reverse movement.
In the event of either switch being pressed, the following warning will appear:
10. MAINTENANCE
--------------------------------------------------------------------------------
The rail guide and the lead screw should be sparingly lubricated periodically;
every 200 hrs of usage. The rail guide and the lead screw should be lubricated
with super lube synthetic grease provided with the pump.
To clean the exterior surfaces, use a lint-free cloth to remove loose dust. Use
care to avoid scratching the clear display window. For more efficient cleaning,
use a soft cloth dampened (not soaked) with water, an aqueous solution of 75%
isopropyl alcohol, or a mild detergent.
11. TROUBLESHOOTING
--------------------------------------------------------------------------------
Table 11.1. Troubleshooting guidelines for the Ossila Syringe Pump
Problem Possible Cause Action No power/display The power switch on the unit is
in the OFF position. Check the connection and ensure the power is turned ON. The
power supply may not be connected properly. Ensure the unit is firmly plugged in
to the power supply and the plug is firmly connected to both the adapter and the
working power socket. The fuse on the rear panel has blown. Ensure the unit is
unplugged. Check the fuse on the rear panel. If it has blown, replace with a
suitably rated 1A slow blow fuse. If the unit was supplied with a transformer,
it may be faulty. Contact Ossila for a replacement transformer. Fault on circuit
board. If all the above causes have been considered, there may be a fault on the
control board. Please contact Ossila for information Motor Stalled (Syringe
Pump) Syringe has hit first safety switch Syringe plunger hitting the bottom / a
kink in the tubing (occlusion) / Syringe plunger binding / Low force setting
Crash Warning The secondary safety switch has accidentally been triggered Turn
the unit off and on again to see if the warning remains The syringe holder is
triggering the secondary safety switch Rotate the lead screw by hand to move the
syringe holder closer to the centre of travel Internal fault in the secondary
safety switch If all the above has been considered there may be a fault with the
secondary safety switch; contact Ossila for more information
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