pages.10xgenomics.com Open in urlscan Pro
104.17.72.206  Public Scan

Form analysis
3 forms found in the DOM

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Text Content

WEBINAR


10X TALK TUESDAY - EXPLORING MECHANISMS DRIVING PEDIATRIC LEUKEMIAS AT
SINGLE-CELL RESOLUTION




 

 
Block 1
editable text

PRESENTED BY 10X GENOMICS

Leukemia accounts for nearly one third of pediatric cancer diagnoses. Next
generation sequencing approaches have led to the identification and
characterization of leukemic subtypes, leading to improved treatments and
outcomes. However, the molecular mechanisms underlying development and
progression of some cases remain inscrutable. To better understand drivers of
pediatric leukemias, we performed single-cell RNA sequencing on a diverse cohort
of pediatric leukemia samples (n = 72), including B-cell acute lymphoblastic
leukemia, T-cell acute lymphoblastic leukemia, acute myeloid leukemia, and mixed
phenotype leukemia. Using the 10x Chromium system, we generated single-cell RNA
sequencing data for 183,321 cells from cryopreserved blood and bone marrow
samples collected from patients at diagnosis, remission, and/or relapse.
Comparative analysis reveals transcriptional markers defining each leukemia,
including PRDM2 and ID3 for B-cell acute lymphoblastic leukemia, PRDM16 and
CSF3R for acute myeloid leukemia, as well as SKI and PTPRC for mixed phenotype
leukemia. Integration of leukemia transcriptomic data with cell atlases
corresponding to healthy blood cells reveals fundamental differences in gene
expression programs between cancer cells and their normal counterparts.
Additionally, this analysis demonstrates the high degree of similarity between
leukemic blasts and hematopoietic stem cells (HSCs), particularly at relapse.
While 45% of B-cell acute lymphoblastic leukemia cells have an HSC-like
transcriptome at diagnosis, this number rises to 97% at relapse, suggesting a
higher degree of plasticity. Future work will explore the mechanistic
significance of these findings, and further explore transcriptional markers with
prognostic value across pediatric leukemias. A deeper understanding of the
molecular drivers involved will ultimately lead to improvements in diagnosis,
patient stratification, and treatment selection for children diagnosed with
leukemia.








FEATURING

IRINA PUSHEL

Senior Bioinformatic Scientist
Children’s Mercy Research Institute




REGISTER NOW

DATE: FEBRUARY 7, 2023

TIME: 10:00 AM - 11:00 AM | CT

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