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Submitted URL: https://cq28b04.na1.hubspotlinksstarter.com/Ctc/2L+113/cQ28b04/VWr_5S7JRrL0W8L0BYL4Z8WsZW1YzZHS52jpsrN6Q02sq3m2ndW7Y8-PT6lZ3mHW7NVnZN3_kQP_W...
Effective URL: https://www.biosafe.fi/laboratory-services/cytotoxicity-tests?utm_medium=email&_hsmi=270478099&_hsenc=p2ANqtz--n7RECYh4...
Submission: On August 17 via manual from IN — Scanned from DE
Effective URL: https://www.biosafe.fi/laboratory-services/cytotoxicity-tests?utm_medium=email&_hsmi=270478099&_hsenc=p2ANqtz--n7RECYh4...
Submission: On August 17 via manual from IN — Scanned from DE
Form analysis 2 forms found in the DOM
POST https://forms.hsforms.com/submissions/v3/public/submit/formsnext/multipart/6777226/8cdbdb27-f5ea-4ea9-9b87-c10f1bc8201b
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<option value="persona_1">I need a marketing authorization for my own products (EFSA)</option>
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<legend class="hs-field-desc" style="display: none;"></legend>
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CYTOTOXICITY TESTS
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DOWNLOAD THE BROCHURE: CYTOTOXICITY TESTS
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DOWNLOAD THE BROCHURE: CYTOTOXICITY TESTS
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also to our newsletter.
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and insights from the world of food safety.
WHY?
The possible toxicity of a microbial strain is usually assessed based on a
literature search and on the whole genome sequence. The presence of genes that
encode virulence factors may trigger further phenotypic testing.
> FOR BACILLUS SPECIES, A SPECIFIC CYTOTOXICITY TEST IS PERFORMED TO DETERMINE
> IF THE PARTICULAR STRAIN PRODUCES HIGH LEVELS OF NON-RIBOSOMALLY SYNTHESISED
> PEPTIDES; THIS IS ONE OF THE QUALIFICATIONS OF THE QPS APPROACH.
The use of strains belonging to the Bacillus cereus group is not advisable in
food or feed applications.
HOW?
BIOSAFE PERFORMS IN VITRO CYTOTOXICITY TESTS WITH VERO CELLS, AS RECOMMENDED BY
EFSA.
The release of lactate dehydrogenase (LDH) is used as a marker of plasma
membrane disruption caused by toxins. Culture supernatants are used as samples.
For this, the customer is instructed to send cell-free culture supernatants from
three independent fermentation batches as well as culture medium to be used as a
control. Exceptionally, the supernatant can be prepared at Biosafe by making a
small-scale culture in the laboratory. EFSA has given guidance for the
preparation of the supernatant samples.
OTHER TESTS
In addition to the LDH release assay, Biosafe has other in vitro toxicity tests,
which can be used for various samples. These include, for example, the boar
spermatozoan motility inhibition test or the Highest Tolerated Dose test (HTD).
The TPC (total protein concentration) test can be used to assess acute
cytotoxicity. Some of the microbial testing available at Biosafe are
specifically designed for the safety assessment of food contact materials.
SAVE 1 000 € ON EACH CYTOTOXICITY TEST IN AUGUST
This August we are offering a discount of 1 000 € on each cytotoxicity test. If
you order your tests in August and send your Bacillus supernatant samples in
September, you will qualify for the discount and get the results by September
29th.
1. Schedule: supernatant samples at Biosafe on the week commencing September
11th, tests on September week 38.
2. Samples: supernatant samples in triplicate (6h cultivation in BHI broth, at
30 degrees °C) and the BHI broth as the negative control.
If a strain is sent instead of the supernatant to Biosafe, the price for sample
preparation is 310 €/strain and the strain should be at Biosafe before September
8th.
CONTACT US
JOUNI HEIKKINEN
Key Account Manager, PhD
+35840 016 5829
jouni.heikkinen@biosafe.fi
MEERI SUTINEN
Quality Manager, Project Manager, PhD
+35840 574 9191
meeri.sutinen@biosafe.fi
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