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bioRxiv posts many COVID19-related papers. A reminder: they have not been
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New Results Follow this preprint
DECODING COVID-19 MRNA VACCINE IMMUNOMETABOLISM IN CENTRAL NERVOUS SYSTEM: HUMAN
BRAIN NORMAL GLIAL AND GLIOMA CELLS BY RAMAN IMAGING
View ORCID ProfileH. Abramczyk, B. Brozek-Pluska, View ORCID ProfileKarolina
Beton
doi: https://doi.org/10.1101/2022.03.02.482639
This article is a preprint and has not been certified by peer review [what does
this mean?].
H. Abramczyk
1Lodz University of Technology, Institute of Applied Radiation Chemistry,
Laboratory of Laser Molecular Spectroscopy, Wróblewskiego 15, 93-590 Łódź,
Poland
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* ORCID record for H. Abramczyk
* For correspondence: halina.abramczyk@p.lodz.pl
B. Brozek-Pluska
1Lodz University of Technology, Institute of Applied Radiation Chemistry,
Laboratory of Laser Molecular Spectroscopy, Wróblewskiego 15, 93-590 Łódź,
Poland
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Karolina Beton
1Lodz University of Technology, Institute of Applied Radiation Chemistry,
Laboratory of Laser Molecular Spectroscopy, Wróblewskiego 15, 93-590 Łódź,
Poland
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10002019601 Comments0 TRiP Peer Reviews0 Community Reviews0 Automated
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ABSTRACT
The paper presents the effect of COVID-19 mRNA (Pfizer/BioNT) vaccine on in
vitro glial cells of the brain studied by means of Raman spectroscopy and
imaging.. The results obtained for human brain normal and tumor glial cells of
astrocytes, astrocytoma, glioblastoma incubated with the Covid-19 mRNA vaccine
Pfizer/BioNT vaccine show alterations in the reduction-oxidation pathways
associated with Cytochrome c.
We found that the Pfizer/BioNT vaccine down regulate the concentration of
cytochrome c in mitochondria upon incubation with normal and tumorous glial
cells. Concentration of oxidized form of cytochrome c in brain cells has been
shown to decrease upon incubation the mRNA vaccine. Lower concentration of
oxidized cytochrome c results in lower effectiveness of oxidative
phosphorylation (respiration), reduced apoptosis and lessened ATP production.
Alteration of Amide I concentration, which may reflect the decrease of mRNA
adenine nucleotide translocator. Moreover, mRNA vaccine leads to alterations in
biochemical composition of lipids that suggest the increasing role of signaling.
mRNA vaccine produce statistically significant changes in cell nucleus due to
histone alterations. The results obtained for mitochondria, lipid droplets,
cytoplasm may suggest that COVID-19 mRNA (Pfizer/BioNT) vaccine reprograms
immune responses. The observed alterations in biochemical profiles upon
incubation with COVID-19 mRNA in the specific organelles of the glial cells are
similar to those we observe for brain cancer vs grade of aggressiveness.
COMPETING INTEREST STATEMENT
The authors have declared no competing interest.
Paper in collection COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv
Copyright
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bioRxiv a license to display the preprint in perpetuity. All rights reserved. No
reuse allowed without permission.
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Decoding COVID-19 mRNA Vaccine Immunometabolism in Central Nervous System: human
brain normal glial and glioma cells by Raman imaging
H. Abramczyk, B. Brozek-Pluska, Karolina Beton
bioRxiv 2022.03.02.482639; doi: https://doi.org/10.1101/2022.03.02.482639
This article is a preprint and has not been certified by peer review [what does
this mean?].
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Decoding COVID-19 mRNA Vaccine Immunometabolism in Central Nervous System: human
brain normal glial and glioma cells by Raman imaging
H. Abramczyk, B. Brozek-Pluska, Karolina Beton
bioRxiv 2022.03.02.482639; doi: https://doi.org/10.1101/2022.03.02.482639
This article is a preprint and has not been certified by peer review [what does
this mean?].
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