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THE NANOIMAGER


THE COMPLETE PACKAGE FOR
SUPER-RESOLUTION MICROSCOPY

 * Features
 * Overview
 * Spec
 * Software

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Nanoimager

CODI Software

Techniques

Resource Library


KEY FEATURES

Our combination of imaging modalities and advanced software allows single
molecules to be observed and tracked with high sensitivity.

Rapid and precise stabilization of the microscope focus, with locking technology
to minimize Z-drift.

Post acquisition drift correction with nanometer precision

Precise control and measurement of temperature, with heating elements to keep
the entire microscope unit at a stable, uniform temperature.
Ideal for live cell imaging, facilitating single-particle tracking.

Single molecule localization microscopy in multiple colors and 2 channels.
Up to 1W laser power at the source.
8-12 kW/cm² power density at the sample plane.

Powerful solutions to control sample conditions.
DNA paint and RNA hybridization.
Up to 16 channels.
Automation of sample preparation.


TECHNIQUES


DSTORM / PALM

Get up to 10 times better resolution than widefield microscopy. Resolving
structures down to 20 nm


SINGLE-PARTICLE TRACKING (SPT)

Follow particles on the nanometer scale simultaneously in two channels.


SINGLE-MOLECULE FRET (SMFRET)

A real-time nanoscale ruler operating on a 2-10 nm range.




ILLUMINATION MODES

SUPER-RESOLUTION IN MANY FORMS

Enhance the quality of your image by changing the illumination angle at the
click of a button

EPIFLUORESCENCE/ WIDEFIELD

Epifluorescence microscopy passes light through the sample in a straight angle,
maximizing the amount of illumination (it is also referred to as widefield
microscopy)

Learn more

TIRF

TIRF microscopy uses the phenomenon of total internal reflection in order to
reduce background noise

Learn more

HILO

HILO microscopy directs the laser at a sharp angle through the sample. This
affords an imaging depth of up to 10µm, at a signal to background noise ratio
(SNR) only slightly lower than that of TIRF.

Learn more



QUICK OVERVIEW

NANOIMAGER S

4

Lasers

1.6

RMS read noise

2

Channels

3D

Included

NANOIMAGER S



4

Lasers

1.6

RMS read noise

2

Channels

3D

Included


FULL SPECIFICATIONS

Download full spec FAQs

 

NANOIMAGER S

IMAGING TECHNOLOGY

2D and 3D single-molecule localization microscopy (SMLM) 3D single-particle
tracking (SPT) Total internal reflection fluorescence (TIRF) Förster resonance
energy transfer (FRET)    

SPATIOTEMPORAL RESOLUTION

Up to 20nm in XY, 50nm in Z via SMLM Up to 10ms / 100Hz with SPT, FRET, TIRF    

IMAGING CHANNELS

4 interlaced, 2 simultaneous <10nm channel mapping resolution    

FIELD OF VIEW

50μm x 80μm with a 100x objective 250μm x 400μm with a 20x objective    

ILLUMINATION

405nm, 488nm, 561nm, 640nm with up to 1W Up tp 10 kW/cm² in the sample plane
(integrated power sensors) Flat-field homogeneous laser illumination Continuous
illumination angle from epi, to HILO to TIRF    

LIGHT PATTERNING

Digital micromirror device (DMD) based light patterning    

OBJECTIVES

Numerical aperture (NA) fom 0.45 to 1.50 Magnification from 20x to 100x    

DIMENSIONS

Nanoimager

21.5cm (w) x 21.5cm (d) x 15.5cm (h)

Light Engine

21.5cm (w) x 42.0cm (d) x 45.0cm (h)

   

Nanoimager S

IMAGING TECHNOLOGY


2D and 3D single-molecule localization microscopy (SMLM)
3D single-particle tracking (SPT)
Total internal reflection fluorescence (TIRF)
Förster resonance energy transfer (FRET)

SPATIOTEMPORAL RESOLUTION


Up to 20nm in XY, 50nm in Z via SMLM
Up to 10ms / 100Hz with SPT, FRET, TIRF

IMAGING CHANNELS


4 interlaced, 2 simultaneous
<10nm channel mapping resolution

FIELD OF VIEW


50μm x 80μm with a 100x objective
250μm x 400μm with a 20x objective

ILLUMINATION


405nm, 488nm, 561nm, 640nm with up to 1W
Up tp 10 kW/cm² in the sample plane (integrated power sensors)
Flat-field homogeneous laser illumination
Continuous illumination angle from epi, to HILO to TIRF

LIGHT PATTERNING


Digital micromirror device (DMD) based light patterning

OBJECTIVES


Numerical aperture (NA) fom 0.45 to 1.50
Magnification from 20x to 100x

DIMENSIONS


21.5cm (w) x 21.5cm (d) x 15.5cm (h)
21.5cm (w) x 42.0cm (d) x 45.0cm (h)


DOWNLOAD FULL TECHNICAL SPECIFICATION

Download the full Nanoimager technical specifications as a PDF

Download

EXPLORE ONI

Software
Super-resolution microscopy



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