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Pierce™ Bradford Plus Protein Assay Kits

Please note the name of Cat. No. 23236 has changed from "Pierce Coomassie Plus
(Bradford) Assay Kit" to "Pierce Bradford Plus Protein Assay Kit". This update
is a name change only, no other changes were made to this product.
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Thermo Scientific™


PIERCE™ BRADFORD PLUS PROTEIN ASSAY KITS

The Pierce Bradford Plus Protein Assay Kit is a ready-to-use, reducing
agent-compatible, improved Bradford assay reagent used to quickly measure total
protein concentration compared to a protein standard.
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Catalog NumberDescriptionKit Contents23236Pierce Bradford Plus Protein Assay
Kit10 x 1 mL glass ampulesA55866Pierce Bradford Plus Protein Assay Kit with
Dilution-Free BSA Protein Standards2 x 1 mL 8-channel tubestrip, prediluted

2 Options
Catalog number 23236


Price (USD)/ 950 mL
228.65
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250.00 
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Description:
Pierce Bradford Plus Protein Assay Kit
Kit Contents:
10 x 1 mL glass ampules
Recurring order eligible. Learn more »
Request bulk or custom format
Price (USD)/ 950 mL
228.65
Online exclusive
250.00 
Save 21.35 (9%)
Add to cart
Pierce™ Bradford Plus Protein Assay Kits
Catalog number23236
Price (USD)/ 950 mL
228.65
Online exclusive
250.00 
Save 21.35 (9%)

In stock
Add to cart

The Pierce Bradford Plus Protein Assay Kit is a ready-to-use, reducing
agent-compatible, improved Bradford assay reagent used to quickly measure total
protein concentration compared to a protein standard. It provides increased
linearity and half the protein-to-protein variability of other commercial
Bradford assay formulations.

Kits are available with or without Pierce Dilution-Free BSA Protein Standards,
which are a set of seven pre-diluted BSA standards, packaged in a multichannel
pipette-friendly tubestrip. The tubestrip includes a single empty tube that
enables users to add their own sample buffer for the purpose of blank
subtraction.

The Pierce Bradford Plus Protein Assay Kit is a ready-to-use, reducing
agent-compatible, improved Bradford assay reagent used to quickly measure total
protein concentration compared to a protein standard. It provides increased
linearity and half the protein-to-protein variability of other commercial
Bradford assay formulations.

Kit options include Pierce Dilution-Free BSA Protein Standards, which are a set
of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly
tubestrip. The tubestrip includes a single empty tube that enables users to add
their own sample buffer for the purpose of blank subtraction.

Features of the Pierce Bradford Plus Protein Assay Kit include:

 * Colorimetric—measure with a standard spectrophotometer or plate reader at 595
   nm
 * Easy to use—single reagent; no working reagent preparation required
 * Fast—almost immediate color development; add, mix, and read results
 * Wide assay range—detects protein concentration in the range 1 to 1500 μg/mL
 * Linear—improved linearity and response uniformity compared to traditional
   Bradford formulations
 * Flexible—microplate and cuvette protocols provided and adaptable to several
   target working ranges
 * Serial dilution–free—select kit options include Dilution-Free BSA Protein
   Standards that eliminate the need to perform serial dilutions when generating
   a standard curve

The assay is performed at room temperature. Simply add the sample to the tube or
well containing reagent, and the resultant blue color is measured at 595 nm
following a short room-temperature incubation. The protein assay is compatible
with most salts, solvents, buffers, thiols, reducing substances, and
metal-chelating agents encountered in protein samples. The assay can be
performed in either test tube or microplate format.

How the Bradford Plus Assay detects protein

Use of Coomassie G-250 dye in a colorimetric reagent for the detection and
quantitation of total protein was first described by Dr. Marion Bradford in
1976. In the acidic environment of the reagent, protein binds to the Coomassie
dye. This results in a spectral shift from the reddish/brown form of the dye
(absorbance maximum at 465 nm) to the blue form of the dye (absorbance maximum
at 610 nm). The difference between the two forms of the dye is greatest at 595
nm, so that is the optimal wavelength to measure the blue color from the
Coomassie dye-protein complex. If desired, the blue color can be measured at any
wavelength between 575 nm and 615 nm. At the two extremes (575 nm and 615 nm)
there is a loss of about 10% in the measured amount of color (absorbance)
compared to that obtained at 595 nm.

Development of color in Coomassie dye-based (Bradford) protein assays has been
associated with the presence of certain basic amino acids (primarily arginine,
lysine, and histidine) in the protein. Van der Waals forces and hydrophobic
interactions also participate in the binding of the dye by protein. The number
of Coomassie dye ligands bound to each protein molecule is approximately
proportional to the number of positive charges found on the protein. Free amino
acids, peptides, and low molecular weight proteins do not produce color with
Coomassie dye reagents. In general, the mass of a peptide or protein must be at
least 3000 Daltons to be assayed with this reagent.

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only.
Specifications
ConcentrationBSA: 2 mg/mL
DescriptionPierce Bradford Plus Protein Assay Kit
AssayBradford Assay
Detection MethodColorimetric
Product LinePierce™
Product TypeProtein Quantitation Assay
SpecificityNot Target-Specific
Sufficient For630 Tube Assays or 3160 Microplate Assays
For Use With (Application)Solution-based Detection, Absorbance
For Use With (Equipment)Spectrophotometer, Microplate Reader
Kit Contents10 x 1 mL glass ampules
Quantity950 mL
Unit Size950 mL
Contents & Storage
Sufficient For: 630 tube assays or 3160 microplate assays
• Pierce Bradford Plus Assay Reagent, 950 mL
• Albumin Standard ampules, 2 mg/mL, 10 x 1 mL

Store Bradford Plus Assay Reagent at 4°C. Store unopened Albumin Standard
ampules at room temperature.


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Featuring higher throughput and built-in cooling for consistent protein transfer
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FIGURES

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CUSTOMERS WHO VIEWED THIS ITEM ALSO VIEWED

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Pierce™ Bradford Plus Protein Assay Reagent
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Pierce™ BCA Protein Assay Kit
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Micro BCA™ Protein Assay Kit
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Pierce™ Bovine Serum Albumin Standard Ampules, 2 mg/mL
Catalog number: 23209
88.50  / 10 x 1 mL
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Pierce™ Bradford Plus Protein Assay Kit with Dilution-Free™ BSA Protein
Standards, multichannel pipette compatible
Catalog number: A55866
294.00  / 950 mL
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Pierce™ Detergent Compatible Bradford Assay Kit
Catalog number: 23246
218.00  / 300 test tube assays
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Pierce™ BCA Protein Assay Kit with Dilution-Free™ BSA Protein Standards,
multichannel pipette compatible
Catalog number: A55864
310.00  / 1 L
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Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible
Catalog number: 23250
319.00  / 275 mL
Add to cart
Pierce™ 660nm Protein Assay Reagent
Catalog number: 22660
203.00  / 750 mL
Add to cart

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DOCUMENTS & DOWNLOADS


CERTIFICATES

Search by lot number or partial lot number

Search
Lot #Certificate TypeDateCatalog Number(s)
ZI392800Certificate of AnalysisOct 21, 202423236
ZH395971Certificate of AnalysisAug 27, 2024A55866
ZH391534Certificate of AnalysisAug 27, 202423236
ZG395969Certificate of AnalysisAug 07, 2024A55866
ZG394725Certificate of AnalysisJul 18, 2024A55866
5 results displayed, search above for a specific certificate
Request a Certificate


SAFETY DATA SHEETS

SDS


SCIENTIFIC RESOURCES

Tech Tip: Protein quantitation assay compatibility table
Tech Tip: How to use a protein assay standard curve
Tech Tip: Determine acceptable wavelengths for measuring protein assays
Tech Tip: Quantitate immobilized protein


PRODUCT INFORMATION

User Guide: Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA
Protein Standards, Multichannel Pipette Compatible
User Guide: Pierce Bradford Plus Protein Assay Kit


FREQUENTLY ASKED QUESTIONS (FAQS)

The assay is compatible with many agents that are incompatible in other assays.
It has been shown to be compatible with amine-containing buffers, reducing
agents, chaotropic agents, organic solvents, sugars, antimicrobial agents, DNA,
protease inhibitors, and low concentrations of metal chelators and metals.

Find additional tips, troubleshooting help, and resources within our Protein
Assays and Analysis Support Center.

We offer several types of protein assays including the: BCA Assay, BCA-RAC
(Reducing Agent Compatible) Assay, Micro BCA Assay, 660 nm Protein Assay, Pierce
Bradford Plus Protein Assay Kit, Pierce Bradford Protein Assay Kit, Modified
Lowry Assay, colorimetric and fluorometric Peptide Assays, CBQCA kit, EZQ kit,
Quant-iT kits, NanoOrange, and the Qubit kits.

Find additional tips, troubleshooting help, and resources within our Protein
Assays and Analysis Support Center.

No. Contrary to what many people assume, it is neither necessary nor even
helpful to know the actual amount (e.g., micrograms) of protein applied to each
well or cuvette of the assay. The amount of protein per well is almost certainly
not the value of interest; instead, one usually wants to know the protein
concentration of the original test sample.

Find additional tips, troubleshooting help, and resources within our Protein
Assays and Analysis Support Center.

There are several criteria that should be considered, including compatibility
with the sample type and components, assay range and required sample volume,
protein-to-protein uniformity, speed and convenience for the number of samples
to be tested, and the availability of spectrophotometer or plate reader
necessary to measure the color produced (absorbance) by the assay.

Find additional tips, troubleshooting help, and resources within our Protein
Assays and Analysis Support Center.

 * Identically assayed samples are directly comparable: Sample assay responses
   are directly comparable to each other if they are processed in exactly the
   same manner. Variation in amount of protein is the only possible cause for
   differences in final absorbance (color intensity) if the samples are
   dissolved in the same buffer, the same lot and stock solution of assay
   reagent is used, all samples are mixed and incubated at the same time and
   temperature, and no pipetting errors were introduced. 
 * Units in equals the units out: The unit of measure used to express the
   standards is by definition the same unit of measure associated with the
   calculated value for the unknown sample (i.e., final results for unknown
   samples will be expressed in the same unit of measure as was used for the
   standards).



Find additional tips, troubleshooting help, and resources within our Protein
Assays and Analysis Support Center.






CITATIONS & REFERENCES (10)

Search citations by name, author, journal title or abstract text

Search
Citations & References
Abstract

Co-association of methotrexate and SPIONs into anti-CD64 antibody-conjugated
PLGA nanoparticles for theranostic application.
Authors:Moura CC, Segundo MA, Neves Jd, Reis S, Sarmento B
Journal:
PubMed ID:25364249
Rheumatoid arthritis (RA) is an autoimmune disease with severe consequences for
the quality of life of sufferers. Regrettably, the inflammatory process involved
remains unclear, and finding successful therapies as well as new means for its
early diagnosis have proved to be daunting tasks. As macrophages are strongly
associated with RA ... More

Differences in sperm protein abundance and carbonylation level in bull
ejaculates of low and high quality.
Authors:Mostek A, Westfalewicz B, Slowinska M, Dietrich MA, Judycka S, Ciereszko
A
Journal:PLoS One
PubMed ID:30427859
'In breeding and insemination centres, significant variation in bull ejaculate
quality is often observed between individuals and also within the same
individual. Low-quality semen does not qualify for cryopreservation and is
rejected, generating economic loss. The mechanisms underlying the formation of
low-quality ejaculates are poorly understood; therefore, the aim of ... More

Mitogen-Activated Protein Kinase Cross-Talk Interaction Modulates the Production
of Melanins in Aspergillus fumigatus.
Authors:Manfiolli AO, Siqueira FS, Dos Reis TF, Van Dijck P, Schrevens S,
Hoefgen S, Föge M, Straßburger M, de Assis LJ, Heinekamp T, Rocha MC, Janevska
S, Brakhage AA, Malavazi I, Goldman GH, Valiante V
Journal:MBio
PubMed ID:30914505
'The pathogenic fungus'

ICAM3-Fc Outperforms Receptor-Specific Antibodies Targeted Nanoparticles to
Dendritic Cells for Cross-Presentation.
Authors:Cruz LJ, Tacken PJ, van der Schoot JMS, Rueda F, Torensma R, Figdor CG
Journal:Molecules
PubMed ID:31083610
'Optimal targeting of nanoparticles (NP) to dendritic cells (DCs) receptors to
deliver cancer-specific antigens is key to the efficient induction of
anti-tumour immune responses. Poly (lactic-co-glycolic acid) (PLGA)
nanoparticles containing tètanus toxoid and gp100 melanoma-associated antigen,
toll-like receptor adjuvants were targeted to the DC-SIGN receptor in DCs by
specific humanized ... More

Metabolic shift from glycogen to trehalose promotes lifespan and healthspan in
Authors:Seo Y, Kingsley S, Walker G, Mondoux MA, Tissenbaum HA
Journal:Proc Natl Acad Sci U S A
PubMed ID:29511104
As Western diets continue to include an ever-increasing amount of sugar, there
has been a rise in obesity and type 2 diabetes. To avoid metabolic diseases, the
body must maintain proper metabolism, even on a high-sugar diet. In both humans
and ... More

10 total citations


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