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* EasySep™
EASYSEP™ CELL ISOLATION & CELL SEPARATION TECHNOLOGY
EASYSEP™ CELL SEPARATION
A SMARTER, MORE EFFICIENT WAY TO ISOLATE CELLS
Working efficiently is key to overcoming the demands of scientific research.
That’s why our scientists developed a smarter, more efficient way to isolate
cells. EasySep™ combines the specificity of monoclonal antibodies with the
simplicity of a column-free magnetic system—for fast and easy isolation of
highly purified cells that are ready for downstream applications. This versatile
immunomagnetic cell separation technology enables positive selection, negative
selection, or cell depletion.
EasySep™ can be used to isolate cells in as little as 8 minutes from a variety
of species (e.g. human, mouse, non-human primate, rat, rabbit, cow and pig) and
sample sources (e.g. splenocytes, lymph nodes, PBMCs, whole blood, leukopaks,
cord blood, and bone marrow).*
View All EasySep™ Cell Separation Kits >
HOW DOES EASYSEP™ CELL SEPARATION WORK?
Cells are targeted for either removal (negative selection and depletion) or
positive selection using antibody complexes directed to specific cell surface
antigens. The antibody complexes link targeted cells to EasySep™ magnetic
particles. Labeled cells are pulled to the sides of the tube when the sample is
placed in an EasySep™ cell separation magnet, while untouched cells can be
simply poured or pipetted off into a new tube.
Request a Sample
WHY USE EASYSEP™ TO ISOLATE CELLS?
* FAST AND EASY. Isolate cells in as little as 8 minutes with a simple pour.
* HIGH PURITY. Achieve up to 99% cell purities with high recoveries.
* GENTLE. Obtain viable, functional cells without the need for columns and
washes.
* VERSATILE. Isolate cells from virtually any sample source, including whole
blood and Leukopaks.
See the Data
IS EASYSEP™ A WELL-ACCEPTED CELL ISOLATION METHOD?
Since its launch in 2002, EasySep™ has been used in thousands of labs worldwide
to quickly and easily isolate highly purified cells for a wide variety of
applications. More immunologists are making the switch to EasySep™ and freeing
up time to accomplish more in the lab. Switching your cell isolation technology
to EasySep™ is easier than you think.
View Frequently Asked Questions
Hear from EasySep™ Users
Read Publication Highlights
We use EasySep™ because of its ease of use and the high purity that we get from
the preparation. I especially like the negative selection kits because it allows
us to keep the cells that we collect as undisturbed as possible
Eric, MD/PhD Student
Already an EasySep™ user? Explore our next-generation EasySep™ kits and make
sure your lab is using the most up-to-date kits.
EasySep™ Human Cell Separation Kits EasySep™ Mouse and Rat Cell Separation Kits
EasySep™ for Other Species and Cell Types Automated Cell Separation
EASYSEP™ HUMAN CELL SEPARATION KITS
View Products
Previous
EASYSEP™ HUMAN CELL ISOLATION KITS
TYPICAL SAMPLE SOURCE:
PBMCs, Leukopaks*
PROTOCOL TIME:
As Little As 8 Minutes
SELECTION METHOD:
Negative Selection
FEATURES/ADVANTAGES:
* High purity (up to 98%)
* Column-free
* Untouched cells immediately ready for downstream analysis
* Can be fully automated using RoboSep™
View Products
Learn More >
Request a Sample >
EASYSEP™ HUMAN POSITIVE SELECTION KITS
TYPICAL SAMPLE SOURCE:
PBMCs, Leukopaks*
PROTOCOL TIME:
As Little As 15 Minutes
SELECTION METHOD:
Positive Selection
FEATURES/ADVANTAGES:
* High purity (up to 99%)
* Column-free
* Can be fully automated using RoboSep™
View Products
Request a Sample >
EASYSEP™ DIRECT CELL ISOLATION KITS
TYPICAL SAMPLE SOURCE:
Whole Blood*
PROTOCOL TIME:
As Little As 20 Minutes
SELECTION METHOD:
Negative Selection
FEATURES/ADVANTAGES:
* 99.9% RBC depletion without density gradient centrifugation, lysis or
sedimentation
* High purity (up to 99%)
* Column-free
* Untouched cells immediately ready for downstream analysis
View Products
Learn More >
Request a Sample >
EASYSEP™ RELEASE CELL SEPARATION KITS
TYPICAL SAMPLE SOURCE:
PBMCs, Leukopaks*
PROTOCOL TIME:
As Little As 29 Minutes
SELECTION METHOD:
Positive Selection
FEATURES/ADVANTAGES:
* High purity (up to 99%)
* Column-free
* Magnetic particle-free cells
* Flexibility to perform sequential positive selections to isolate unique and
rare cell types
View Products
Learn More >
Request a Sample >
Next
Expand Details
Collapse Details
*Certain products are only available in select territories. Please contact your
local Sales representative or the Product & Scientific Support team at
techsupport@stemcell.com for further information.
They are very efficient kits and I can perform my isolations quickly without
compromising on cell quality or viability.
Payel, Postdoctoral Fellow
ON-DEMAND HUMAN IMMUNE CELL ISOLATION COURSE
Learn how to effectively process human samples and isolate highly pure target
cells with EasySep™ in this free self-paced course.
View Course >
EASYSEP™ MOUSE AND RAT CELL SEPARATION KITS
View All EasySep™ Products for Mouse Cell Separation Kits
View All EasySep™ Products for Rat Cell Separation Kits
EASYSEP™ MOUSE CELL ISOLATION KITS
TYPICAL SAMPLE SOURCE:
Spleen, Bone Marrow, Whole Blood, Lymph Nodes
PROTOCOL TIME:
As Little As 15 Minutes
SELECTION METHOD:
Negative Selection
FEATURES/ADVANTAGES:
* Fast and easy
* High purity (up to 98%)
* Column-free
* Untouched cells immediately ready for downstream analysis
* Can be fully automated using RoboSep™
View Products
Learn More >
Request a Sample >
EASYSEP™ MOUSE POSITIVE SELECTION KITS
TYPICAL SAMPLE SOURCE:
Spleen, Bone Marrow, Whole Blood, Lymph Nodes
PROTOCOL TIME:
As Little As 15 Minutes
SELECTION METHOD:
Positive Selection
FEATURES/ADVANTAGES:
* Fast and easy
* High purity (up to 99%)
* Column-free
* Can be fully automated using RoboSep™
View Products
Request a Sample >
EASYSEP™ RAT CELL ISOLATION KITS
TYPICAL SAMPLE SOURCE:
Spleen, Lymph Nodes, Whole Blood
PROTOCOL TIME:
As Little As 13 Minutes
SELECTION METHOD:
Negative Selection
FEATURES/ADVANTAGES:
* Fast and easy
* High purity (up to 99%)
* Column-free
* Untouched cells immediately ready for downstream analysis
* Can be fully automated using RoboSep™
View Products
Request a Sample >
Expand Details
Collapse Details
I currently use the EasySep™ Mouse T Cell Isolation Kit, CD4+ T Cell Isolation
Kit, and CD8+ T Cell Isolation Kit. I have used different isolation kits over
the years and these yield superior results, in my opinion.
Cori, PhD Student
EASYSEP™ FOR OTHER SPECIES AND CELL TYPES
EasySep™ cell separation technology can also be used to isolate cells from a
variety of other species, including non-human primate, rabbit, cow and pig. If
you are working with specific rare cell types, non-standard species, or special
applications, you can explore our custom cell isolation solutions or contact us
at info@stemcell.com to discuss your specific cell separation needs.
View all products and try EasySep™ in your own lab.
Request a Sample
AUTOMATED CELL SEPARATION
EasySep™ cell separation technology can be automated using RoboSep™. Minimize
sample handling and perform simultaneous and sequential cell isolations from
multiple samples using RoboSep™-S and RoboSep™-16 instruments.
Learn More >
RELATED RESOURCES
MAGNETIC CELL SEPARATION
Find answers to three commonly asked questions about immunomagnetic cell
separation.
Read Now
CHOOSING A POSITIVE OR NEGATIVE SELECTION APPROACH
Read more about antibody labeling-based cell separation methods, including
immunomagnetic and immunodensity cell separation.
Learn More
FIND THE RIGHT ANTIBODY FOR YOUR APPLICATIONS
Use our Antibody Finder Tool to select high-quality primary and secondary
antibodies verified to work with EasySep™ products in specific applications.
View Tool
Brochure
Tools for Your Immunology Research
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
14:21
Video
How to Isolate Immune Cells from Large-Volume Samples Using the Easy 250
EasySep™ Magnet
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
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