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 * 17994 STUDY

 * PRODUCTS

 * TEST PRINCIPLE

 * APPLICATION

 * COMPLIANCE

 * QUALITY

 * DISTRIBUTION

 * CONTACT

ENUMERATION OF ESCHERICHIA COLI AND COLIFORM BACTERIA IN 18 HOURS

COLIKAT® RAPID is a fast test to simultaneously detect both total coliforms and
Escherichia coli in water samples providing the test’s results within 18 hours.
COLIKAT® RAPID is suited for the entire water matrix.

The water samples to be tested are incubated overnight so that they are ready to
read early the next day. Therefore COLIKAT® RAPID delivers significant workflow
benefits and speed in the event of a contamination issue. The test’s high
sensitivity enables early coliform detection in water distribution systems,
assisting to help to reduce biofilm buildup and sporadic downstream
contamination events. As compared to the alternative conventional method using
Chromogenic Coliform Agar (CCA; ISO 9308-1) for detection, COLIKAT® RAPID
significantly reduces false alarms created by inaccurate false positive results
which give rise to uncertainty and unnecessary expense.

 * Simultaneous detection and quantification of Escherichia coli and total
   coliforms for the entire water matrix.
 * Sample evaluation after 18 hours.
 * Optimized workflow in your laboratory: The sample evaluation takes place
   after 18 hours before the reception of next days’ samples.
 * Enumeration of Escherichia coli and coliform bacteria in water samples acc.
   to EN ISO 9308-2.

 * Product data sheet
 * Product features
 * Instructions for use

 * Product Range
 * Sicherheitsdatenblatt
 * Safety Data Sheet

Leitungswasser

TAP WATER

Trinkwasser

BOTTLED WATER

Meerwasser

SEA WATER

Badewasser

BATHING WATER

Kaerwasser

WASTE WATER

Grundwasser_1

SURFACE WATER


SUMMARY OF EN ISO 17994 STUDY RESULTS:

> “The study clearly proved that the XEBIOS‘s COLIKAT® RAPID method is
> equivalent to the IDEXX‘s COLILERT-18/QUANTI-Tray® method for the enumeration
> of coliform bacteria. 
> For the target organism E. coli the study clearly proved that XEBIOS‘s
> COLIKAT® RAPID has a significantly higher recovery compared to IDEXX‘s
> reference method COLILERT- 18®/QUANTI-Tray®.” 

IWW Rheinisch-Westfälisches Institut für Wasser Beratungs- und
Entwicklungsgesellschaft mbH, January 2021 [Final report of a comparison study
for the evaluation of the equivalence of XEBIOS DIAGNOSTICS COLIKAT RAPID® and
IDEXX LABORATORIES COLILERT-18®, page 18], Download here.

 * IWW Rheinisch-Westfälisches Institut für Wasser Beratungs- und
   Entwicklungsgesellschaft mbH as independent and internationally recognized
   experts have carried out a comparison study on behalf of Xebios to evaluate
   the equivalence of Colikat Rapid® and Idexx Laboratories Inc. Colilert-18® as
   reference method.
 * The study has been carried out in accordance with the requirements of EN ISO
   17994:2014: Water Quality – Requirements for the comparison of the relative
   recovery of microorganisms by two quantitative methods.
 * 522 sample-pairs were used for the comparison of the test systems’
   performance for the enumeration of the key target organism Escherichia coli,
   470 sample-pairs for coliform bacteria were included into the study –
   providing a profound database to guarantee statistically sound statements
   within EN ISO 17994’s test design.

The study showed that
 * Colikat Rapid® has a significantly higher recovery for E. coli compared
   to Idexx Laboratories Inc. Colilert-18®
 * both methods are equivalent regarding the enumeration of coliform bacteria.

Download
Complete study - EN (PDF)
Download
Komplette Studie - DE (PDF)


PRODUCTS AVAILABLE NOW


COLIKAT RAPID

Learn more


COLIKAT RAPID

 * Suitable for presence-/absence testing and simultaneous identification and
   quantification of Escherichia coli and coliform bacteria using the COLIKAT
   Blister
 * Easy to use without extensive training of laboratory staff in the laboratory
   or in the field
 * Clear identification of target organisms avoids results’ bias resulting from
   subjective interpretation
 * No confirmation testing required
 * Detection of 1 CFU/100ml water sample
 * 18 hours test period perfectly fits into laboratory workflow and helps to
   avoid night and weekend work
 * 24 months shelf life at room temperature
 * Low percentage of false positive results increases security and avoids
   unnecessary cost
 * Quality tested according to EN ISO 11133 and EN ISO 9308-2 in our EN ISO
   17025 accredited laboratory
 * Test is compliant with §15 Sec. 1a Nr. 1 of TrinkWV and European Union
   Directive 2015/1787 EC


COLIKAT
ENUMERATION TRAY

Learn more


COLIKAT ENUMERATION TRAY

 * For the simultaneous identification and quantification of Escherichia coli
   and coliform bacteria
 * 3-side pre-sealing insures easy filling of the COLIKAT Enumeration Tray
 * Easy and fast sealing to separate the cavities with the COLIKAT Seal
   Applicator

Download Enumeration Tray 51 (PDF)
Download Enumeration Tray 97 (PDF)


XDG MULTIPLE WELL MPN TRAY SEALER

Learn more


XDG MULTIPLE WELL MPN TRAY SEALER

 * Compact sealer to seal the COLIKAT Enumeration Tray
 * Safe separation of the cavities for valid quantification


COLIKAT
SAMPLE VESSELS

Learn more


COLIKAT SAMPLE VESSELS

 * 125ml and 300ml vessels
 * Available with and without sodium thiosulfate
 * Available with and without anti-foaming agent


COLIKAT
COMPARE KIT

Learn more


COLIKAT COMPARE KIT

 * To assist distinguishing positive from negative results by comparing the
   color of the Compare Kit with the color of the sample


QC
STRAINS

Learn more


QC STRAINS

 * Escherichia coli (WDCM 00090)
 * Escherichia coli (WDCM 00013)
 * Klebsiella pneumoniae (WDCM 00206)
 * Pseudomonas aeruginosa (WDCM 00024)

Code Product name Details 95-1901 COLIKAT RAPID® (18h) 100 tests for 100ml
samples in stand-up pouch 95-1902 COLIKAT® Enumeration Tray 51 10 Enumeration
Trays in bag 95-1904 COLIKAT® Enumeration Tray 97 10 Enumeration Trays in bag
95-1909 COLIKAT® Antifoam Agent 20ml for 200 tests 95-1910 Sample vessels 120ml
w. sodium thiosulfate, shrink band 100 vessels, sterile, 100ml mark 95-1911
Sample vessels 120ml, shrink band 100 vessels, sterile, 100ml mark 95-1912
Sample vessels 120ml, tear-of-label 100 vessels, sterile, 100ml mark 95-1913
Sample vessels 120ml w. sodium thiosulfate, tear-of-label 100 vessels, sterile,
100ml mark 95-1915 COLIKAT® Comperator Enumeration Tray 51 1 Comperator to
compare samples 95-1916 COLIKAT® Comperator Enumeration Tray 97 1 Comperator to
compare samples 95-1917 COLIKAT Comperator Vessel 1 Sample Vessel 120ml 95-1924
COLIKAT® 24 100 tests for 100ml samples 95-1930 COLIKAT® RTU 25 tests for 100ml,
reagent in vessel 95-1931 COLIKAT® RTU 25 tests for 100ml, reagent w/
thiosulfate in vessel 95-1934 COLIKAT RAPID® RTU KIT 100 tests for 100ml
(COLIKAT RAPID, 51 tray, vessel) 95-1935 COLIKAT RAPID® RTU KIT 100 tests for
100ml (COLIKAT RAPID, 97 tray, vessel) 95-1940 ENTEROKAT (coming soon) 25 tests
for 100ml samples 95-1941 ENTEROKAT (coming soon) 100 tests for 100ml samples
95-1950 XDG Multiple Well MPN Tray Sealer 1 thermal sealing device to seal MPN
Enumeration Tra

Download
Product Range (PDF)
Download MPN table
for Enumeration Tray 51 (PDF)
Download MPN table
for Enumeration Tray 97 (PDF)


PRODUCTS BENEFITS:

 * Significantly higher recovery for the key target organism Escherichia coli
 * 1:1 equivalent results regarding coliform bacteria
 * The COLIKAT RAPID® reagent dissolves significantly faster in the sample
 * No antifoam is needed for the majority of water samples

 * Saves more then 80% volume of waste and storage area for the COLIKAT RAPID®
   reagent 
 * Manufactured and distributed by your reliable service partners Xebios
   Diagnostics GmbH and our local established and experienced distribution
   partners

Play Video about colikat imagefilm vorschaubild 1


TEST PRINCIPLE

COLIKAT RAPID® uses certain specific characteristics of both Escherichia
coli and coliform bacteria for identification of the species. The test contains
MUG and ONPG, two common nutritional indicators. Coliform bacteria use their
enzyme β-Galactosidase to metabolize ONPG turning the sample yellow. Escherichia
coli – other then most coliform bacteria – additionally has the enzyme
β-Glucuronidase that enables it to hydrolyze MUG so that the sample additionally
shows fluorescence under UV-light.

Most of non-coliform bacteria possess neither the β-Galactosidase nor
the β-Glucuronidase. Therefore, they cannot transform MUG and ONPG and the test
will not detect them. Yet, some non-coliform bacteria contain the enzymes.
COLIKAT RAPID® therefore uses selective supplements that inhibit the growth of
those bacteria. The combination of the test’s enzymes and selective supplements
prevents some common problems of conventional culture methods such as Chromogen
Coliform Agar CCA (EN ISO 9308-1):

 * False positive results are prevented (for example for Aeromonas spec.)
 * The growth of non-target organism is prohibited. This way non-target
   organisms cannot affect (or even inhibit) the growth of Escherichia coli and
   coliform bacteria


APPLICATION – INSTRUCTIONS FOR USE

Seal the Enumeration Tray with the XDG Multiple Well MPN Tray Sealer or another
enumeration tray sealing device.

Incubate the sample 18-22 hours at 36°C ± 2°C. Count the yellow wells of COLIKAT
Enumeration Tray (= coliforms). Count the yellow wells of the COLIKAT
Enumeration Tray that show fluorescence under an UV light (365nm) in a dark
environment (= Escherichia coli).

Transform the count results to the number of CFU the sample by using the MPN
table in the appendix to EN ISO 9308-2.

Presence/ Absence: Add 1 bag of COLIKAT RAPID® to 100 ml of water sample. Close
the sample vessle and shake until the reagent dissolves.

For presence/absence testing incubate the closed vessel for 18-22 hours at 36°C
± 2°C and read-off the result. The vessels should have room temperature before
incubation. If turned yellow coliforms are present. If it shows ﬂuorescence
unter UV-light Escherichia coli is present.

Quantification: For quantitative testing pour the 100ml sample into the COLIKAT
Enumeration Tray.

Seal the Enumeration Tray with the XDG Multiple Well MPN Tray Sealer or another
enumeration tray sealing device.

Incubate the sample 18-22 hours at 36°C ± 2°C. Count the yellow wells of COLIKAT
Enumeration Tray (= coliforms). Count the yellow wells of the COLIKAT
Enumeration Tray that show fluorescence under an UV light (365nm) in a dark
environment (= Escherichia coli).

Transform the count results to the number of CFU the sample by using the MPN
table in the appendix to EN ISO 9308-2.

Presence/ Absence: Add 1 bag of COLIKAT RAPID® to 100 ml of water sample. Close
the sample vessle and shake until the reagent dissolves.

For presence/absence testing incubate the closed vessel for 18-22 hours at 36°C
± 2°C and read-off the result. The vessels should have room temperature before
incubation. If turned yellow coliforms are present. If it shows ﬂuorescence
unter UV-light Escherichia coli is present.

Quantification: For quantitative testing pour the 100ml sample into the COLIKAT
Enumeration Tray.




REGULATORY ENVIRONMENT AND COMPLIANCE

COLIKAT RAPID® is a fast test to simultaneously detect both total coliforms
and Escherichia coli in water samples. The testing method has been described as
ISO standard for detecting total coliforms and Escherichia coli in water (ISO
9308-2). In European Union (EU) Directive 2015/1787 EC requires the member
states to – implement ISO 9308-2 into national legislation.

The requirements for the quality of water intended for human consumption within
the European Union are bindingly set by EU regulation:

 * DIRECTIVE 98/83/EC – quality of water intended for human consumption
 * DIRECTIVE 2015/1787 EC amending Annexes II and III to Council Directive
   98/83/EC on the quality of water intended for human consumption

A number of ISO standards have been established for analyzing microbiological
parameters. EN ISO 9308-1 and EN ISO 9308-2 (for the enumeration of Escherichia
coli and coliform bacteria) provide all necessary specifications for performing
the analysis of Escherichia coli and coliform bacteria. The actual standards to
be applied, reflecting the latest scientific and technical progress, are
reflected by the amendments to Annex III of Directive 98/83/EC. The
microbiological parameters and methods of analysis are specified in Annex III to
Directive 98/83/EC that has been amended as follows by Directive  2015/1787 EC:

“The methods for microbiological parameters are:

(a) Escherichia coli (E. coli) and coliform bacteria
     (EN ISO 9308-1 or EN ISO 9308-2)
(b) Enterococci (EN ISO 7899-2)
(c) Pseudomonas aeruginosa (EN ISO 16266)
(d) enumeration of culturable microorganisms — colony count 22 °C
     (EN ISO 6222)
(e) enumeration of culturable microorganisms — colony count 36 °C
     (EN ISO 6222)
(f) Clostridium perfringens including spores (EN ISO 14189)’;

The directive requires the member states to implement ISO 9308-2 into national
legislation: According to Directive 2015/1787 EC, “Member States shall bring
into force the laws, regulations and administrative provisions necessary to
comply with this Directive by 27 October 2017 at the latest. They shall
forthwith communicate to the Commission the text of those provisions.” As a
result of the European legislation, all member states will have to accept EN ISO
9308-2 as method for the analysis of water samples of water intended for human
consumption to ensure compliance with EU legislation. Germany has already
implemented the new EU regulation into national law:

In Germany, the relevant law to comply with Directive 2015/1787 EC is the
“Trinkwasserverordnung” (TrinkWV): “Diese Verordnung dient der Umsetzung der
Richtlinie (EU) 2015/1787 der Kommission vom 06. Oktober 2015 zur Änderung der
Anhänge II und III der Richtlinie 98/83/EG des Rates über die Qualität von
Wasser für den menschlichen Gebrauch.“

The TrinkWV has been adapted to comply with the new EU regulation (with delay)
on 03. January 2018. The new TrinkWV has been published in the
“Bundesgesetzblatt” and therefore has become effective on 08. January 2018.
According to § 15 (1a) Nr. 1 the following methods are to be used for the
analysis of drinking water: “Coliforme Bakterien und Escherichia coli (E. coli):
DIN EN ISO 9308-1:2017-09 oder DIN EN ISO 9308-2:2014-06.”

DIN EN ISO 9308-2:2014-06 states

(a) the typical composition of the medium to be used.
(b) that the circumstance, that the method has been named as  branded  by its
inventor, does clearly not state exclusivity to produce and use the formulation
described by EN ISO 9308-2.


QUALITY

The manufacturing, storage and quality control of COLIKAT RAPID® is carried out
by Xebios Diagnostics GmbH in compliance with EN ISO 11133 and considering Good
Manufacturing Practice (GMP). The quality management system is ISO
9001 certified. The quality testing of COLIKAT RAPID® takes place in our EN ISO
17025 accredited laboratory complying with all requirements of EN ISO 11133 and
EN ISO 9308-2. It is our goal to meet our customers’ high quality requirements
at all time.

Our quality management system includes broad testing procedures for sterility,
stability and shelf live, the microbiological performance and physical condition
of our products. We trust only established partners which have proven to
delivery according to our high quality standards for years for the sourcing of
our raw materials and technical equipment.

Xebios Diagnostics GmbH
Certificate ISO 9001 Xebios Diagnostics GmbH


DISTRIBUTION PARTNERS

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CONTACT AND SUPPORT

Please contact us at any time for all your questions about our products and
services as well as for an individual quotation.

You can reach our team by phone, e-mail or by using our contact form below.

Xebios Diagnostics GmbH
Telleringstraße 49
40597 Düsseldorf

T : +49 211 78 88 07 – 00
E : sales@xebios.de


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COLIKAT RAPID® COLIKAT Enumeration Tray XDG Multiple Well MPN Tray Sealer
COLIKAT Sample Vessels COLIKAT Compare Kit QR Strains

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