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Effective URL: https://kromatid.com/gene-editing-services/
Submission: On August 15 via manual from US — Scanned from DE
Effective URL: https://kromatid.com/gene-editing-services/
Submission: On August 15 via manual from US — Scanned from DE
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* Human Whole Chromosome Paints
* Subcentromere Pinpoint FISH™ Probes
* Subtelomere Pinpoint FISH™ Probes
* Oncology Pinpoint FISH™ Probes
* FISH KITS
* TP53/CEP17 Pinpoint FISH™ Kit
* dGH™ PROBESNEW!
* Subcentromere dGH in-Site™ Probes
* Subtelomere dGH in-Site™ Probes
* DGH™ KITS
* dGH in-Site™ CAR-T Probes
* dGH™ Assay Cell Prep Kit
* BUFFERS AND REAGENTS
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* Custom Plasmid ManufacturingNEW!
* Routine Plasmid Preps – Plasmid ProsNEW!
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* Cytogenetic Assays for Genotoxicity and Quality ControlNEW!
* dGH in-Site™ Targeted Assay Services
* dGH SCREEN™ High Resolution Karyotyping
* Pinpoint FISH™ Assay Services
* Radiation Biodosimetry Services
* RESEARCH SERVICES
* Cell Culture Services
* G-Banding Services
* SUPPORT & FAQ’s
* TECHNICAL INFORMATION
* dGH™ Method Application Note
* Microscope Specifications Data Sheet
* Water Bath Application Notes for Pinpoint FISH™ Probes
* Multiplexing with Pinpoint FISH™ Probes Application Notes
* First Time Users Application Notes for Pinpoint FISH™ Probes
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* Subtelomere Pinpoint FISH™ Probes
* Oncology Pinpoint FISH™ Probes
* FISH KITS
* TP53/CEP17 Pinpoint FISH™ Kit
* dGH™ PROBESNEW!
* Subcentromere dGH in-Site™ Probes
* Subtelomere dGH in-Site™ Probes
* DGH™ KITS
* dGH in-Site™ CAR-T Probes
* dGH™ Assay Cell Prep Kit
* BUFFERS AND REAGENTS
* SERVICES
* PLASMID SERVICES
* Custom Plasmid ManufacturingNEW!
* Routine Plasmid Preps – Plasmid ProsNEW!
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* Cytogenetic Assays for Genotoxicity and Quality ControlNEW!
* dGH in-Site™ Targeted Assay Services
* dGH SCREEN™ High Resolution Karyotyping
* Pinpoint FISH™ Assay Services
* Radiation Biodosimetry Services
* RESEARCH SERVICES
* Cell Culture Services
* G-Banding Services
* SUPPORT & FAQ’s
* TECHNICAL INFORMATION
* dGH™ Method Application Note
* Microscope Specifications Data Sheet
* Water Bath Application Notes for Pinpoint FISH™ Probes
* Multiplexing with Pinpoint FISH™ Probes Application Notes
* First Time Users Application Notes for Pinpoint FISH™ Probes
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* PRODUCTS
* FISH PROBES
* Human Whole Chromosome Paints
* Subcentromere Pinpoint FISH™ Probes
* Subtelomere Pinpoint FISH™ Probes
* Oncology Pinpoint FISH™ Probes
* FISH KITS
* TP53/CEP17 Pinpoint FISH™ Kit
* dGH™ PROBESNEW!
* Subcentromere dGH in-Site™ Probes
* Subtelomere dGH in-Site™ Probes
* DGH™ KITS
* dGH in-Site™ CAR-T Probes
* dGH™ Assay Cell Prep Kit
* BUFFERS AND REAGENTS
* SERVICES
* PLASMID SERVICES
* Custom Plasmid ManufacturingNEW!
* Routine Plasmid Preps – Plasmid ProsNEW!
* ASSAY SERVICES
* Cytogenetic Assays for Genotoxicity and Quality ControlNEW!
* dGH in-Site™ Targeted Assay Services
* dGH SCREEN™ High Resolution Karyotyping
* Pinpoint FISH™ Assay Services
* Radiation Biodosimetry Services
* RESEARCH SERVICES
* Cell Culture Services
* G-Banding Services
* SUPPORT & FAQ’s
* TECHNICAL INFORMATION
* dGH™ Method Application Note
* Microscope Specifications Data Sheet
* Water Bath Application Notes for Pinpoint FISH™ Probes
* Multiplexing with Pinpoint FISH™ Probes Application Notes
* First Time Users Application Notes for Pinpoint FISH™ Probes
* PRESENTATIONS & POSTERS
* Scientific Presentations
* Posters
* FREQUENTLY ASKED QUESTIONS
* ABOUT KROMATID
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* Who We Are
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* FISH PROBES
* Human Whole Chromosome Paints
* Subcentromere Pinpoint FISH™ Probes
* Subtelomere Pinpoint FISH™ Probes
* Oncology Pinpoint FISH™ Probes
* FISH KITS
* TP53/CEP17 Pinpoint FISH™ Kit
* dGH™ PROBESNEW!
* Subcentromere dGH in-Site™ Probes
* Subtelomere dGH in-Site™ Probes
* DGH™ KITS
* dGH in-Site™ CAR-T Probes
* dGH™ Assay Cell Prep Kit
* BUFFERS AND REAGENTS
* SERVICES
* PLASMID SERVICES
* Custom Plasmid ManufacturingNEW!
* Routine Plasmid Preps – Plasmid ProsNEW!
* ASSAY SERVICES
* Cytogenetic Assays for Genotoxicity and Quality ControlNEW!
* dGH in-Site™ Targeted Assay Services
* dGH SCREEN™ High Resolution Karyotyping
* Pinpoint FISH™ Assay Services
* Radiation Biodosimetry Services
* RESEARCH SERVICES
* Cell Culture Services
* G-Banding Services
* SUPPORT & FAQ’s
* TECHNICAL INFORMATION
* dGH™ Method Application Note
* Microscope Specifications Data Sheet
* Water Bath Application Notes for Pinpoint FISH™ Probes
* Multiplexing with Pinpoint FISH™ Probes Application Notes
* First Time Users Application Notes for Pinpoint FISH™ Probes
* PRESENTATIONS & POSTERS
* Scientific Presentations
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* FREQUENTLY ASKED QUESTIONS
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Genome Editing ServicesDave Burdick2023-07-12T16:02:14-06:00
DGH IN-SITE™ ASSAYS
TRACK YOUR GENOME EDITING OUTCOMES WITH THE HIGHEST RESOLUTION
DGH IN-SITE™ CAN DETECT AND DIFFERENTIATE ANY GENOMIC TARGET INCLUDING
INVERSIONS STARTING AT 2KB. FOR GENE THERAPY APPLICATIONS, IN-SITE™ IS DESIGNED
TO TRACK AND LOCATE TRANSGENE INTEGRATION THROUGHOUT THE GENOME.
Key benefits of dGH in-Site™ assays:
* Track transgenes and define Insertional Copy Number (ICN).
* On- and off-target Insertional Copy Number (ICN).
* Characterize structural variations from mis-repair of your edit sites.
* Distribution of integrations on both a single cell and genome-wide basis.
Customized to meet individual genomic structural research needs unique to your
project.
For edit site applications in-Site™ is designed for analysis of specific edits
or engineered cell lines to measures any structural variation involved with the
edit site, including rearrangements involving on-target and off-target loci.
Available for any sequenced mammalian genome including human, animal models and
bio-production cell lines.
* 1/4 -EXAMPLE OF IN-SITE AND SCREEN COMBINATION ASSAY IN UNEDITED HUMAN
FIBROBLAST CONTROL LINE CELL. BRACKETING IN-SITE PROBES ON CHROMOSOME 22
(CIRCLED) TO IDENTIFY INSERT TARGET LOCATION AND DGH SCREEN PAINTS ON
CHROMOSOMES 1, 2, AND 3 (PINK). NO INSERT WOULD BE EXPECTED IN THIS CONTROL
LINE
* 2/4 - EXAMPLE OF A SINGLE CELL FROM THE SAME IN-SITE ASSAY IN AN EDITED IPSC
VIEWED IN MULTIPLE FLUORESCENCE CHANNELS. LEFT IMAGE SHOWS ALL FLUORESCENCE
CHANNELS OVERLAID, MIDDLE SHOWS YELLOW/INSERT PROBE CHANNEL, AND RIGHT SHOWS
GREEN/ON-TARGET BRACKETING PROBE CHANNEL. MULTIPLE INSERTS ARE VISIBLE
OUTSIDE OF THE ON-TARGET BRACKETING PROBES, INDICATING HIGH LEVELS OF
OFF-TARGET INTEGRATIONS IN THIS CELL.
* 3/4 - EXAMPLE OF THE SAME IN-SITE ASSAY IN AN EDITED IPSC LINE CELL WITH NO
OFF TARGET INSERTIONS. TARGET SITE BRACKETING PROBES IN GREEN AND INSERT
PROBE IN YELLOW ZOOMED IN AND EXPANDED ON THE RIGHT. RESULTS FROM THIS SAMPLE
INDICATED THAT 6% OF CELLS HAD NO OFF-TARGET INSERTIONS.
* 4/4 - IMAGE FROM A DGH IN-SITE ASSAY IN POLYPLOID, DUAL-EDITED, HEK-293FT
CELLS DEMONSTRATING BOTH NORMAL AND REARRANGED EDIT SITE STRUCTURES. AT THE
NORMAL EDIT SITES (A), BOTH SETS OF IN-SITE PROBES ARE LOCATED ON THE SAME
SIDE OF THE CHROMOSOME. AN INVERSION AT THE EDIT SITE IS ALSO VISIBLE (B),
WITH ONE PORTION OF THE IN-SITE PROBE SWITCHING ORIENTATIONS AND SHOWING UP
ON THE OPPOSITE CHROMATID FROM THE OTHER PORTIONS OF THE IN-SITE PROBE. A
PARTIAL DELETION AT ONE EDIT SITE IS ALSO OBSERVED (C), WITH THE RED PORTION
OF THE IN-SITE PROBES MISSING.
*
*
REQUEST QUOTE
ORDERING INFORMATION
CATALOG
PRODUCTS
LIST PRICE
DGH-001 in-Site Adherent/Suspension Culture Development: Thaw, recovery, and
harvest optimization $1,250.00 DGH-002 in-Site Standard Probe Production: Design
and verification of standard probes $1,155.00 DGH-003 in-Site Assay Execution
and Analysis: Imaging and scoring for 4 probe assay, 200 cells counted per
sample $8,163.75 DGH-004 in-Site Assay Execution and Analysis: Imaging and
scoring for 3 probe assay, 200 cells counted per sample $6029.10 DGH-005 in-Site
Assay Execution and Analysis: Imaging and scoring for 2 probe assay, 200 cells
counted per sample $4,874.10 DGH-006 in-Site Assay Execution and Analysis:
Imaging and scoring for 1 probe assay, 200 cells counted per sample $3,719.00
DGH-007 in-Site Custom Probe Production: Design and verification of custom
probes $6,250.00 DGH-008 in-Site Adherent/Suspension Metaphase Prep and Harvest
$1,250.00 DGH-009 in-Site T-Cell Culture Development: Thaw, recovery, and
harvest optimization $1,500.00 DGH-010 in-Site IPSC Cell Culture Development:
Thaw, recovery, and harvest optimization $1,625.00 DGH-011 in-Site Whole Blood
Culture Development: Thaw, recovery, and harvest optimization $1,125.00 DGH-012
in-Site T Cells Metaphase Prep and Harvest $1,500.00 DGH-013 in-Site IPSC
Metaphase Prep and Harvest $1,625.00 DGH-014 in-Site Whole Blood Metaphase Prep
and Harvest $1,125.00 DGH-015 in-Site Assay Execution and Analysis: Imaging and
scoring for 4 probe assay, 20 cells counted per sample $1,224.56 DGH-016 in-Site
Assay Execution and Analysis: Imaging and scoring for 4 probe assay, 50 cells
counted per sample $2,551.17 DGH-017 in-Site Assay Execution and Analysis:
Imaging and scoring for 4 probe assay, 20 cells counted per sample with two
quadrants per slide $795.97 DGH-018 in-Site Assay Execution and Analysis:
Imaging and scoring for 4 probe assay, 50 cells counted per sample with two
quadrants per slide $1,658.26 DGH-019 in-Site Assay Execution and Analysis:
Imaging and scoring for 4 probe assay, 20 cells counted per sample with four
quadrants per slide $517.38 DGH-020 in-Site Assay Execution and Analysis:
Imaging and scoring for 4 probe assay, 50 cells counted per sample with four
quadrants per slide $1,077.87 DGH-021 dGH in-Site™ Whole Blood Metaphase Prep –
Elongated $1,687.50 DGH-022 dGH in-Site™ Assay Execution and Analysis: Imaging
and scoring for 3 probe assay, 100 cells counted per sample $3,738.04 DGH-023
dGH in-Site™ Assay Execution and Analysis: Imaging and scoring for 3 probe
assay, 50 cells counted per sample $1,869.02 DGH-024 dGH in-Site™ NK Cells
Metaphase Prep and Harvest $1,500.00 DGH-025 dGH in-Site™ Assay Execution and
Analysis: Imaging and scoring for 1 probe assay, 100 cells counted per sample
$1,007.50 DGH-026 dGH in-Site™ Calibration per sample $2,062.50 DGH-027 dGH
in-Site™ Assay Execution and Analysis: Imaging and Scoring for 1 probe assay, 50
cells per sample $1,875.00 DGH-028 dGH in-Site™ QC one timepoint test per sample
$625.00 DGH-029 dGH in-Site™ NK Cell Culture Development: Thaw, recovery, and
harvest optimization $1,500.00 DGH-030 dGH in-Site™ Assay Execution and
Analysis: Imaging and scoring for 1 probe assay, 20 cells counted per sample
$1,218.75 DGH-031 dGH in-Site™ Assay Execution and Analysis: Imaging and scoring
for 1 probe assay, 500 cells counted per sample $5,552.93 DGH-032 dGH in-Site™
Assay Execution and Analysis: Imaging and scoring for 1 probe assay, 600 cells
counted per sample $7,496.46 DGH-033 dGH in-Site™ Assay Execution and Analysis:
Imaging and scoring for 2 probe assay, 20 cells counted per sample $1,340.63
DGH-034 dGH in-Site™ Assay Execution and Analysis: Imaging and scoring for 2
probe assay, 50 cells counted per sample $2,010.94 DGH-035 dGH in-Site™ Assay
Execution and Analysis: Imaging and scoring for 2 probe assay, 100 cells counted
per sample $3,016.41 DGH-036 dGH in-Site™ Assay Execution and Analysis: Imaging
and scoring for 2 probe assay, 500 cells counted per sample $6,108.22 DGH-037
dGH in-Site™ Assay Execution and Analysis: Imaging and scoring for 2 probe
assay, 600 cells counted per sample $8,246.10 DGH-038 dGH in-Site™ Assay
Execution and Analysis: Imaging and scoring for 3 probe assay, 20 cells counted
per sample $1,474.69 DGH-039 dGH in-Site™ Assay Execution and Analysis: Imaging
and scoring for 3 probe assay, 500 cells counted per sample $6,719.04 DGH-040
dGH in-Site™ Assay Execution and Analysis: Imaging and scoring for 3 probe
assay, 600 cells counted per sample $9.070.71 DGH-041 dGH in-Site™ Assay
Execution and Analysis: Imaging and scoring for 4 probe assay, 100 cells counted
per sample $3,649.85 DGH-042 dGH in-Site™ Assay Execution and Analysis: Imaging
and scoring for 4 probe assay, 500 cells counted per sample $7,390.95 DGH-043
dGH in-Site™ Assay Execution and Analysis: Imaging and scoring for 4 probe
assay, 600 cells counted per sample $9,977.78
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REQUEST QUOTE
RESOURCES
Data Sheet
Overview
Sample dGH in-Site™ Report
APPLICATION NOTES
dGH™ Method
TECHNICAL RESOURCES & EDUCATION
Genotoxicity Assays Datasheet
Target Structural Variants Poster
CRISPR/CAS9 GENE EDITING POSTER
Automating the Analysis of Genomic Damage
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