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CELLCULTURE


 * MAIN INDEX
 * Introduction
 * Apparatus and Equipment
 * Aseptic technique
 * media and reagents
   * preparation and sterilization
   * culture media
 * culture vessesl
 * SOP & protocols
   * SOP and protocols
   * subculture
   * quantitation
 * contamination

Search for: Search




HEMATOXILIN (MAYER’S) AND EOSIN STAINING OF CULTURED CELLS

Posted on November 18, 2022November 26, 2022
Posted inSenza categoria
Hematoxylin and eosin (H&E) stains have been used for a long time. They are
still widely used to recognize the…


AUTOCLAVE: STEAM STERILIZATION

Posted on October 12, 2022October 13, 2022
Posted inSenza categoria
Of all the methods available for sterilization, moist heat in an autoclave
(under pressure) is one of the most common…


THE MTT ASSAY FOR CELL VIABILITY

Posted on October 6, 2022October 13, 2022
Posted inSenza categoria
Cell viability assays are often used to evaluate the effects of test compounds
to study their cytotoxic effect or whether…


THE LOWRY METHOD TO QUANTIFY PROTEIN

Posted on September 19, 2022September 20, 2022
Posted inquantitation , SOP & protocols
Another way to indirectly estimate the number of cells in a sample is to
evaluate the protein content using Lowry’s…


NEUTRAL RED: DYE UPTAKE VIABILITY TEST (NRU)

Posted on March 18, 2022September 20, 2022
Posted inquantitation , SOP & protocols
Neutral red uptake (NRU assay) is one of the most widely used cytotoxicity and
cell viability tests. Cell viability tests…


ERYTHROSIN B: DYE EXCLUSION VIABILITY TEST

Posted on February 21, 2022March 1, 2022
Posted inquantitation , SOP & protocols
Erythrosin B can be used for cell viability test. Cell viability is an index of
the “health state” of the…


TRYPAN BLUE: DYE EXCLUSION VIABILITY TEST

Posted on February 14, 2022February 15, 2022
Posted inquantitation , SOP & protocols
Trypan Blue is used for the study of cell viability by a method referred to as
exclusion staining. Healthy cells…


THE COMPONENTS OF CELL CULTURE MEDIA: GLUTAMINE

Posted on February 9, 2022March 10, 2022
Posted inmedia and reagents
Glutamine is a non-essential amino acid for the body, however mammalian cells in
culture require the presence of millimolar concentrations…


PREPARATION OF CELL CULTURE MEDIA

Posted on February 3, 2022February 8, 2022
Posted inpreparation and sterilization
The preparation of cell culture media, which must take place under aseptic
conditions, changes a little depending on the initial…


TRYPSINIZATION: PROTOCOL, TIPS AND TRICKS

Posted on January 31, 2022February 8, 2022
Posted inSOP & protocols , subculture
Proteolysis with the use of trypsin – or trypsinization – is a process where you
expose cells to trypsin in…


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SEARCH

Search for: Search


POPULAR POST

 * Hematoxilin (Mayer’s) and Eosin staining of cultured cells
 * Autoclave: steam sterilization
 * The MTT assay for cell viability
 * The Lowry method to quantify protein
 * Neutral red: dye uptake viability test (NRU)




ULTIMI ARTICOLI

 * 3 months ago
   
   HEMATOXILIN (MAYER'S) AND EOSIN STAINING OF CULTURED CELLS

 * 4 months ago
   
   AUTOCLAVE: STEAM STERILIZATION

 * 4 months ago
   
   THE MTT ASSAY FOR CELL VIABILITY

 * 5 months ago
   
   THE LOWRY METHOD TO QUANTIFY PROTEIN

 * 11 months ago
   
   NEUTRAL RED: DYE UPTAKE VIABILITY TEST (NRU)



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 * MAIN INDEX
 * Introduction
 * Apparatus and Equipment
 * Aseptic technique
 * media and reagents
   * preparation and sterilization
   * culture media
 * culture vessesl
 * SOP & protocols
   * SOP and protocols
   * subculture
   * quantitation
 * contamination



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