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Submitted URL:
http://t.spmailhk.com/t.aspx/subid/173589001/camid/2209051/linkid/356711/Default.aspx
Effective URL:
https://shop.biosearchtech.com/nucleic-acid-sample-preparation/dna-and-rna-purification-kits-and-reagents/sbeadex-lightning-nuc...
Submission: On August 12 via api (August 12th 2024, 9:36:23 am UTC) from HK — Scanned from DE

Form analysis
5 forms found in the DOM

Name: search_form_SearchBox — GET /search/

<form name="search_form_SearchBox" class="search_form_SearchBox" method="get" action="/search/" novalidate="novalidate">
  <input type="text" id="js-site-search-input" class="js-site-search-input ui-autocomplete-input" name="text" value="" maxlength="100" placeholder="Search for products, resources and more…"
    data-options="{&quot;appendTo&quot; : &quot;#predictive-search&quot;,&quot;autocompleteUrl&quot; : &quot;/search/autocomplete/SearchBox&quot;, &quot;autocompleteDefaultImg&quot;: &quot;/_ui/responsive/theme-lgc/images/filler-images/filler-image-96.png&quot;,&quot;minCharactersBeforeRequest&quot; : &quot;3&quot;,&quot;waitTimeBeforeRequest&quot; : &quot;500&quot;,&quot;displayProductImages&quot; : true, &quot;labels&quot;: { &quot;pricingUnavailable&quot; : &quot;Pricing unavailable&quot;, &quot;from&quot;: &quot;From&quot; } }"
    autocomplete="off">
  <button class="js-search-button" type="submit" disabled="disabled" aria-label="Search">
    <span></span>
  </button>
  <span class="js-close-search header_search_close"></span>
  <span class="header__search-close--mobile js-close-search">cancel</span>
</form>

POST /p/NAP40-031-01/showmdvariant

<form id="mDVariantSelectionForm" action="/p/NAP40-031-01/showmdvariant" method="post" autocomplete="off" novalidate="novalidate"><input type="hidden" name="productCode" value="NAP40-031-01">
  <div class="box__section">
    <fieldset class="product-variant-selector js-product-variant-selector">
      <legend class="form-legend">
        <em>Option 1:</em>&nbsp;Select a Format
      </legend>
      <div class="product-variant-selector__options">
        <div class="product-variant-selector__option">
          <input class="product-variant-selector__input" type="radio" id="formatEnz_corekita" name="categories[0]" value="formatEnz_corekita" data-option="1" data-relations=" sizeEnz_100pur , sizeEnz_10000pur , sizeEnz_1000pur ">
          <label class="product-variant-selector__label" for="formatEnz_corekita">
            <strong>Core Kit A</strong>
            <!-- <span>Additional info if required</span> -->
          </label>
        </div>
        <div class="product-variant-selector__option">
          <input class="product-variant-selector__input" type="radio" id="formatEnz_corekitb" name="categories[0]" value="formatEnz_corekitb" checked="checked" data-option="1" data-relations=" sizeEnz_100pur , sizeEnz_10000pur , sizeEnz_1000pur ">
          <label class="product-variant-selector__label" for="formatEnz_corekitb">
            <strong>Core Kit B</strong>
            <!-- <span>Additional info if required</span> -->
          </label>
        </div>
      </div>
      <div class="alert alert-danger alert-no-border u-m-0" role="alert" style="display: none;"> Please select a Format</div>
    </fieldset>
  </div>
  <div class="box__section">
    <fieldset class="product-variant-selector js-product-variant-selector">
      <legend class="form-legend">
        <em>Option 2:</em>&nbsp;Select a Size
      </legend>
      <div class="product-variant-selector__options">
        <div class="product-variant-selector__option">
          <input class="product-variant-selector__input" type="radio" id="sizeEnz_100pur" name="categories[1]" value="sizeEnz_100pur" checked="checked" data-option="2" data-relations=" formatEnz_corekita , formatEnz_corekitb ">
          <label class="product-variant-selector__label" for="sizeEnz_100pur">
            <strong>100 purifications</strong>
            <!-- <span>Additional info if required</span> -->
          </label>
        </div>
        <div class="product-variant-selector__option">
          <input class="product-variant-selector__input" type="radio" id="sizeEnz_1000pur" name="categories[1]" value="sizeEnz_1000pur" data-option="2" data-relations=" formatEnz_corekita , formatEnz_corekitb " disabled="disabled">
          <label class="product-variant-selector__label" for="sizeEnz_1000pur">
            <strong>1000 purifications</strong>
            <!-- <span>Additional info if required</span> -->
          </label>
        </div>
        <div class="product-variant-selector__option">
          <input class="product-variant-selector__input" type="radio" id="sizeEnz_10000pur" name="categories[1]" value="sizeEnz_10000pur" data-option="2" data-relations=" formatEnz_corekita , formatEnz_corekitb " disabled="disabled">
          <label class="product-variant-selector__label" for="sizeEnz_10000pur">
            <strong>10000 purifications</strong>
            <!-- <span>Additional info if required</span> -->
          </label>
        </div>
      </div>
      <div class="alert alert-danger alert-no-border u-m-0" role="alert" style="display: none;"> Please select a Size</div>
    </fieldset>
  </div>
  <div>
    <input type="hidden" name="CSRFToken" value="2264fb88-1ea9-4a14-a6c7-ada9f79a822d">
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</form>

POST /cart/add

<form id="addToCartForm" class="add_to_cart_form" action="/cart/add" method="post" novalidate="novalidate"><input type="hidden" name="qty" class="js-qty-selector-input-hidden" value="1">
  <input type="hidden" name="productCodePost" value="NAP40-031-01">
  <input type="hidden" name="productBaseProduct" value="SBXLCK">
  <input type="hidden" name="productBaseProductName" value="sbeadex Lightning Nucleic Acid Purification Kit">
  <button id="addToCartButton" type="submit" class="btn btn-primary btn-block js-add-to-cart js-enable-btn" data-is-scheduled-delivery-cart="false" data-price="" data-should-keep-disabled="false" data-free-item-qty-limit="false"> Add to
    basket</button>
  <div>
    <input type="hidden" name="CSRFToken" value="2264fb88-1ea9-4a14-a6c7-ada9f79a822d">
  </div>
</form>

Name: accessiblityForm —

<form name="accessiblityForm" novalidate="novalidate">
  <input type="hidden" id="accesibility_refreshScreenReaderBufferField" name="accesibility_refreshScreenReaderBufferField" value="">
</form>

/_c/country

<form class="country-select__form js-country-select-form" action="/_c/country" novalidate="novalidate">
  <select name="isoCode" class="form-control">
    <option value="">Please select</option>
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    <option value="DZ">Algeria</option>
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    <option value="BO">Bolivia</option>
    <option value="BQ">Bonaire, Saint Eustatius and Saba</option>
    <option value="BA">Bosnia and Herzegovina</option>
    <option value="BW">Botswana</option>
    <option value="BR">Brazil</option>
    <option value="IO">British Indian Ocean Territory</option>
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    <option value="BG">Bulgaria</option>
    <option value="BF">Burkina Faso</option>
    <option value="BI">Burundi</option>
    <option value="KH">Cambodia</option>
    <option value="CM">Cameroon</option>
    <option value="CA">Canada</option>
    <option value="IC">Canary Islands</option>
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    <option value="KY">Cayman Islands</option>
    <option value="CF">Central African Republic</option>
    <option value="TD">Chad</option>
    <option value="CL">Chile</option>
    <option value="CN">China</option>
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    <option value="CC">Cocos (Keeling) Islands</option>
    <option value="CO">Colombia</option>
    <option value="KM">Comoros</option>
    <option value="CG">Congo</option>
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    <option value="CK">Cook Islands</option>
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    <option value="HR">Croatia</option>
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    <option value="CY">Cyprus</option>
    <option value="CZ">Czech Republic</option>
    <option value="DK">Denmark</option>
    <option value="DJ">Djibouti</option>
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    <option value="DO">Dominican Republic</option>
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    <option value="ER">Eritrea</option>
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    <option value="GE">Georgia</option>
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    <option value="GH">Ghana</option>
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    <option value="GR">Greece</option>
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    <option value="ZM">Zambia</option>
    <option value="ZW">Zimbabwe</option>
    <option value="XI"></option>
  </select>
  <div class="country-select__validation help-block"> Please select your country</div>
  <div class="country-select__btn">
    <button class="btn btn-default" type="submit">Continue</button>
  </div>
</form>

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Quick Order
 1. Home
 2. Nucleic Acid Sample Preparation
 3. DNA and RNA Purification Kits and Reagents
 4. sbeadex Lightning Nucleic Acid Purification Kit





SBEADEX LIGHTNING NUCLEIC ACID PURIFICATION KIT




PRODUCT DETAILS

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The sbeadex Lightning Nucleic Acid Purification Kit comes as a modular system to
tailor your optimal kit. Choose from two versions of sbeadex Lightning, Core Kit
A and Core Kit B. 

Each kit contains:

 * sbeadex Lightning binding buffer (LP in Kit A and LU in Kit B)
 * sbeadex particles suspension
 * elution buffer (AMP)

Core kits can be complemented with a range of individual components (e.g. lysis
buffers, Protease K solution, debris capture beads) that can be purchased
separately. Just buy what you need to establish your optimal purification
method!

We highly recommend starting with our comprehensive starter kit to evaluate
performance and optimise workflow before progressing to one of our core kits and
lysis buffers, especially for those unfamiliar with our product line.  

 


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SBEADEX LIGHTNING NUCLEIC ACID PURIFICATION KIT




SBEADEX LIGHTNING NUCLEIC ACID PURIFICATION CORE KIT B, 100 PURIFICATIONS

The sbeadex Lightning Nucleic Acid Purification Kit is a game-changing
innovation in nucleic acid isolation - as it delivers high-quality DNA in high
yield with a simple, ultrafast 3-step/5-minute protocol, saving time and costs.
It comes as a modular system allowing to compile a kit optimised for a specific
sample type.


KEY FEATURES

Show Hide
 * Save time and increase sample throughput: Superfast and convenient protocols
   that adapt seamlessly to automated high-throughput workflows
 * Flexibility: Optimise nucleic acid purification from different sample types
   using our self-assembly kit solutions (2 core kits, 6 different lysis buffers
   and other optional components). Unsure which to choose? Start with our
   sbeadex Lightning Nucleic Acid Purification Starter Kit
 * Cost-savings: Benefit from a 3-step/5-minute protocol that reduces overall
   lab costs (e.g. plastic consumables) and ensures reliable and reproducible
   results
 * High DNA quality and yield: Attain highly purified DNA in high yields
   suitable for a wide range of downstream applications
 * Convenience and stability: Ready-to-use reagents with high stability at room
   temperature
 * Sustainability and eco-friendliness: New, flexible kit format and shortened
   protocol reduce components to what is actually needed - reducing plastic,
   liquid and packaging waste

Option 1: Select a Format
Core Kit A
Core Kit B
Please select a Format
Option 2: Select a Size
100 purifications
1000 purifications
10000 purifications
Please select a Size

Item ID NAP40-031-01
TBD
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SIMILAR PRODUCTS

DNA and RNA Purification Kits and Reagents


SBEADEX LIGHTNING NUCLEIC ACID PURIFICATION STARTER KIT

The sbeadex Lightning Nucleic Acid Purification Starter Kit is intended for
testing purposes, allowing users to evaluate performance and optimise conditions
and workflow for D…
ITEM ID
NAP40-032-00

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 * 1

Added to basket
 * Product info
 * Specs & Usage
 * Documentation
 * Complete set
 * FAQs


PRODUCT INFORMATION

Explore the versatility of sbeadex Lightning chemistry

Discover the potential with our two core kit options, Core kit A and Core kit B.
Each kit contains everything you need to follow our lightning protocol designed
for efficient 3-step purification of high-quality DNA from plant and animal
tissue lysates in as little as typically 5 minutes. You can complete the Core
Kits by ordering individual components such as recommended lysis buffers,
Protease K or Debris capture beads. Before ordering a Core Kit, we recommend
using our Starter Kit to evaluate and determine the optimal composition of Core
kit and individual components.

Core Kit options

Core Kit A: Recommended for most sample varieties and particularly effective for
plant samples.

Core Kit B: Optimized for specific sample types, especially mammalian tissue
samples.

We highly recommend starting with our comprehensive Starter Kit to evaluate
optimal lysis conditions and performance before progressing to one of our core
kits.

Both kits embrace an environmentally friendly approach that reduces plastics,
packaging, shipping costs, and hazardous waste.

To complete your sbeadex Lightning kit we offer a range of individual components
that allow a flexible workflow optimization tailored to your specific sample
types and needs.

Revolutionary lightning technology

Our patent pending sbeadex Lightning product line brings a new era of nucleic
acid isolation with a superfast and straightforward 3-step protocol. Purify
nucleic acids in just typically 5 minutes from a variety of plant and animal
sample materials. This innovative technology cuts time by requiring only a
binding buffer, our proprietary sbeadex particles, and an elution buffer.
Despite that simplified and shortened protocol, sbeadex Lightning delivers
highly pure DNA in high yields that easily draws level with popular purification
kits. 

Applications

DNA purification: Ideal for downstream applications such as PCR/qPCR,
restriction analysis/cloning or sequencing/NGS.

Wide sample compatibility: Applicable to a diverse range of sample types,
including plant and animal tissues (see product details).

The sbeadex Lightning technology

This kit leverages the power of sbeadex magnetic bead technology. These
superparamagnetic particles, coated with sbeadex surface chemistry, employ a
novel one-step binding mechanism that ensures effective removal of impurities
from the sample matrix. The recommended but optional wash step is performed with
water, preventing organic/alcoholic traces in the eluate that might inhibit
enzymatic downstream applications. This eliminates the need for a drying step
before elution, further enhancing DNA quality.

Read more


PRODUCT SPECIFICATIONS AND USAGE

Automation of sbeadex Lightning 

oKtopure

The sbeadex Lightning DNA purification protocol can be automated using our fully
automated nucleic acid isolation platform, the oKtopure™ platform.

For experienced oKtopure users, the following two links to zip files contain a
run file and three advanced mixing routines, one for each step of the protocol
(bind, wash, elute). These are provided for both 100 µL and 200 µL of lysate
input volume.

The table below provides summary information about the available oKtopure run
files, including the total run time of each.

  100 µL input volume 200 µL input volume Run file sbxlightning_100V_Elu50
sbxlightning_200V_Elu100 Run time (8 plates) 1 hour 36 minutes 1 hour 55 minutes

When establishing sbeadex Lightning on your oKtopure, we recommend liaising with
our automation experts to ensure that all instrument settings are appropriate
for your sample types. Biosearch Technologies offer pilot projects to determine
feasibility for challenging sample types and to address parameters such as
customised sample input amounts and elution volumes.

KingFisher Flex

Automation can also be achieved using the KingFisher Flex platform; we provide
standard protocols that you can access below.

This zip file contains 12 different BindIt files (.bdz) and 12 corresponding
report files (.pdf) that contain general information about the run file, the
reagent information and details regarding the steps of the automated protocol.

To determine the most appropriate BindIt file (.bdz) for your application, you
need to choose:

 1. the relevant input volume – either 100 µL or 200 µL of lysate per well.

 2. the desired elution time – 90 seconds, 300 seconds, or 600 seconds.
    Different plant species and sample types react differently to the elution
    time. In general, longer elution times guarantee that all available DNA is
    eluted, but for some sample types (particularly plants) the shorter elution
    times have shown to be sufficient.

 3. the number of plates to be processed simultaneously – either one plate or
    two plates, depending on your laboratory setup.

The table below provides summary information about the available BindIt files,
including the total run time of each.



 

Alternative liquid handlers can also be used to automate the sbeadex Lightning
chemistry; please contact technical support (techsupport@lgcgroup.com) if you
require any guidance.  

Please note that manually optimised protocols may require further optimisation
when transferring to an automated platform.

 

Read more


DOCUMENTATION


SDS

 * MSDS EN Binding buffer LP
 * MSDS EN Binding buffer LU
 * MSDS EN sbeadex particle suspension
 * MSDS EN Elutions Buffer AMP


PROTOCOLS

 * sbeadex Lightning protocol for plant and livestock tissue DNA purification


MANUALS AND USER GUIDES

 * sbeadex Lightning manual
 * sbeadex Lightning buffer recommendations guide
 * sbeadex Lightning Starter Kit quick guide
 * sbeadex Lightning Hamilton manual


PRODUCT INFORMATION SHEETS

 * sbeadex Lightning data sheet


COMPLETE SET


DO YOU NEED THESE TO COMPLETE YOUR SET?


SBEADEX LIGHTNING NUCLEIC ACID PURIFICATION CORE KIT B IS USUALLY BOUGHT WITH
THESE ITEMS:




FAQS


FAQS




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