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Biochemistry Class notes

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CARDIAC TROPONIN I (CTNI) ASSAY BY IMMUNOCHROMATOGRAPHY METHOD: PRINCIPLE,
PROCEDURE, INTERPRETATION AND RESULTS

🕐 May 24, 2016

Rapid Assay for detection of Human Cardiac Troponin I (cTnl) in Serum/Plasma &
Whole Blood by ImmunoChromatography (adsbygoogle = window.adsbygoogle ||
[]).push({}); Fig. Troponin I kit showing Positive result Principle: This test
utilizes the principle of immunochromatography, with a unique two-site sandwich
immunoassay on a nitrocellulose membrane. The conjugate pad contains two
components - monoclonal anti-cTnl conjugated to colloidal gold and rabbit IgG
conjugated to colloidal gold. A... Read More Visit
http://edusanjalbiochemist.blogspot.com/2016/05/cardiac-troponin-i-ctni-assay-by.html

BCR/ABL1 TESTING: INTRODUCTION, INDICATIONS, METHODS AND INTERPRETATION

🕐 February 28, 2016

(adsbygoogle = window.adsbygoogle || []).push({}); Fig. BCR-ABL1 fusion gene
(Philadelphia chromosome) BCR-ABL1 refers to a gene sequence found in an
abnormal chromosome 22 of some people with certain forms of leukemia. Unlike
most cancers, the cause of chronic myelogenous leukemia (CML) and some other
leukemias can be traced to a single, specific genetic abnormality in one
chromosome. The presence of the gene sequence known as BCR-ABL1 confirm... Read
More Visit
http://edusanjalbiochemist.blogspot.com/2016/02/bcrabl1-testing-introduction.html

ZIKA VIRUS : EPIDEMIOLOGY, SYMPTOMS, DIAGNOSIS, TREATMENT AND PREVENTION

🕐 January 26, 2016

(adsbygoogle = window.adsbygoogle || []).push({}); What is Zika Virus ? Zika
virus falls under member of Flavivirus. The vector for Zika vius is Aedes
mosquito. Zika virus is an emerging mosquito-borne virus that was first
isolated in Zika forest of Uganda in 1947. Since then, it has been observed
mainly in Africa, with small and sporadic outbreaks in Asia. Fig. Zika virus is
transmitted by daytime mosquitoes under the genus Aedes. Credit: Photo f... Read
More Visit
http://edusanjalbiochemist.blogspot.com/2016/01/zika-virus-epidemiology-symptoms.html

PHOTOMETRY: PRINCIPLE, APPLICATIONS AND TYPES

🕐 December 21, 2015

(adsbygoogle = window.adsbygoogle || []).push({}); Photometry Fig. Principle of
colorimetry When light is passed through a coloured solution, certain
wavelengths are selectively absorbed giving a plot of the absorption spectrum of
the compound in solution. The wavelength at which maximum absorption is called
the absorption maximum (λmax) of that compound. The light that is not absorbed
is transmitted through the solution and gives the solution its colour.
Photometric instruments measure... Read More Visit
http://edusanjalbiochemist.blogspot.com/2015/12/photometry-principle-applications-and.html

CEREBROSPINAL FLUID (CSF) : FORMATION, COMPOSITION, FUNCTION, PATHOLOGICAL STATE
AND BIOCHEMICAL ANALYSIS

🕐 December 20, 2015

(adsbygoogle = window.adsbygoogle || []).push({}); A. Formation Cerebrospinal
fluid (CSF) is a clear, colourless fluid filling the ventricles and subarachnoid
space. CSF production is a result of the combined processes of diffusion,
pinocytosis and active transfer. The majority is produced by selective dialysis
of blood plasma by a specialized sponge-like structure called the "choroid
plexus" of third, lateral and fourth ventricles. Fig. CSF collection The anatomy
of the ventricular system... Read More Visit
http://edusanjalbiochemist.blogspot.com/2015/12/cerebrospinal-fluid-csf-formation.html

PROTEIN SEPARATION TECHNIQUES: PROTEIN FRACTIONATION

🕐 December 17, 2015

(adsbygoogle = window.adsbygoogle || []).push({}); Fig. Protein purification
steps Proteins differ in their molecular size and charge. They can be separated
on the basis of their following properties: 1. molecular size 2.
solubility 3. electrical charge 4. adsorption properties 5.
specific bioaffinity. Ma... Read More Visit
http://edusanjalbiochemist.blogspot.com/2015/12/protein-separation-techniques-protein.html

QUALITATIVE AND QUALITATIVE ESTIMATION OF PROTEINS

🕐 December 17, 2015

(adsbygoogle = window.adsbygoogle || []).push({}); Proteins are the most
abundant biomolecules, constituting more than 50% of the dry weight of the cell.
They are known to perform many different biological functions. Qualitative and
quantitative estimation of proteins in biological fluids is based on the
physico-chemical properties of proteins. Denaturation of proteins In each type
of protein, the three dimensional structure is essential for its biological
function. A protein in ... Read More Visit
http://edusanjalbiochemist.blogspot.com/2015/12/qualitative-and-qualitative-estimation.html

TRANSFORMING BLOOD TESTING : ELIZABETH HOLMES INVENTION

🕐 March 31, 2015

(adsbygoogle = window.adsbygoogle || []).push({}); The next time you get a blood
test, you might not have to go to the doctor and watch vials of blood fill up as
the precious fluid is drawn from your arm. No more wondering to yourself, "Ah,
how much more can they take before I pass out?" Instead you might be able to
walk into a Walgreens pharmacy for a reportedly painless finger prick that will
draw just a tiny drop of blood, thanks to Elizabeth Holmes, 30, the youngest
woman and third-youngest billi... Read More Visit
http://edusanjalbiochemist.blogspot.com/2015/03/transforming-blood-testing-elizabeth.html

SWINE FLU: PANIC IN INDIA

🕐 March 01, 2015

Indian Health Minister JP Nadda has urged the public not to panic, as the number
of deaths so far this year from swine flu passed 900 from 16,000 cases.
Rajasthan and Gujarat are among the worst affected states. Officials in
Ahmedabad, Gujarat's largest city, have announced restrictions on public
gatherings as a precautionary measure. The current outbreak, which began in
December last year, is India's deadliest since 2010. Nearly 4,000 people have
been killed in separate outbreaks of the H1N1 ... Read More Visit
http://edusanjalbiochemist.blogspot.com/2015/03/swine-flu-panic-in-india.html

MEGALOBLASTIC ANEMIA: FOLATE AND VITAMIN B12 METABOLISM, CAUSES, DIAGNOSIS AND
THERAPY

🕐 January 31, 2015

Fig. Megaloblastic anemia showing megaloblastics cells MACROCYTOSIS An increased
MCV can be due to a number of reasons but careful review of the patient's
history and blood smear can narrow the diagnostic possibilities. The
differential can be divided into two broad categories based on RBC morphology.
Round macrocytosis-due to abnormal lipid composition of the erythrocyte
membrane. Common etiologies include: 1. Al... Read More Visit
http://edusanjalbiochemist.blogspot.com/2015/01/megaloblastic-anemia-folate-and-vitamin.html

CHRONIC COUGH: MOST COMMON 8 REASONS

🕐 January 21, 2015

(adsbygoogle = window.adsbygoogle || []).push({}); Are you fed up with your
stubborn cough? There are many reasons why a cough may not improve, and that can
be frustrating for people and their doctors alike. Reason 1: Irritated Airways
After a Cold or Flu The most common cause of chronic cough is predictable. It's
the aftermath of a cold or other viral infection, says Norman H. Edelman, MD,
chief medical officer of the American Lung Association. Most cold symptoms may
go away after a few days.... Read More Visit
http://edusanjalbiochemist.blogspot.com/2015/01/chronic-cough-most-common-8-reasons.html

SUBACUTE THYROIDITIS: INTRODUCTION, PATHOGENESIS, SYMPTOMS AND TREATMENT

🕐 December 25, 2014

What Is Subacute Thyroiditis? Thyroiditis refers to an inflamed thyroid.
Your thyroid is a gland in the front of your neck that controls your metabolism
by releasing a variety of hormones. Most types of thyroiditis do not cause pain
in the thyroid gland. However, they typically lead to
either hyperthyroidism (overactive thyroid) or hypothyroidism (underactive
thyroid). Both of these conditions cause symptoms such as weight changes,
anxiety, and fatigue. ... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/12/subacute-thyroiditis-introduction.html

LOW BLOOD SUGAR: WHAT ARE THE EFFECTS IN OUR BODY

🕐 December 16, 2014

Fig. Effects of low blood sugar Every cell in your body needs sugar (glucose) to
function. When your blood sugar levels drop too low, your cells become starved
for energy. Initially, that can cause minor symptoms, but if you don’t get your
blood sugar levels up soon, you’re at risk of serious complications. Effects of
Low Blood Sugar on the Body (adsbygoogle = window.adsbygoogle || []).push({});
When your blood sugar (glucose) levels fall below the normal range, it’s called
hyp... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/12/low-blood-sugar-what-are-effects-in-our.html

BONE METABOLISM : REGULATION (PART 2)

🕐 November 21, 2014

Calcium and phosphate balance Soluble Ca2+, hydroxyapatite and calcitonin As
calcium (Ca2+) is one of the main components of our bones, large amounts are
present in our body. At the same time, comparatively low extracellular
concentrations of Ca2+ are fine-tuned to regulate important functions, not to
speak of even far lower intracellular concentrations. This dichotomy is possible
due to the low solubility product of Ca2+ and phosphate (PO43-): if one ion is
added to a solution of the o... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/11/bone-metabolism-regulation-part-2.html

BONE METABOLISM: BONE FORMATION AND BONE RESORPTION (PART 1)

🕐 November 21, 2014

BONE FORMATION AND BONE RESORPTION Fig. Bone Cells Living bone is constantly
being remodeled. The state of our bones is always close to an equilibrium
between bone formation and bone resorption. In childhood and during the teens,
bone formation is slightly ahead. We reach peak bone mass in the twenties, and
from then onwards, resorption has the upper hand. There are two reasons for the
constant remodeling process. Firstly, it allows our bones to adapt to changes in
load. For example, consider... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/11/bone-metabolism-bone-formation-and-bone.html

CHEMOTHERAPY AND IT'S SIDE EFFECTS

🕐 October 17, 2014

Chemotherapy side effects Chemotherapy drugs kill cancer cells, but they can
also damage healthy cells. This damage to healthy cells causes side effects.
Different cells and tissues in the body tolerate chemotherapy differently.
Chemotherapy drugs have the greatest effect on rapidly dividing cells, such as
blood cells in the bone marrow, cells lining the mouth and gastrointestinal (GI)
tract and hair follicle cells. (adsbygoogle = window.adsbygoogle ||
[]).push({}); What causes side effects? ... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/10/chemotherapy-and-its-side-effects.html

EXPERIENCES FROM EBOLA OUTBREAK ZONE

🕐 October 17, 2014

Interview with Photojournalist Neil Brandvold Q&A: What It’s Like to Cover Ebola
After returning from West Africa, photojournalist Neil Brandvold is isolating
himself as a precaution. Photojournalist Neil Brandvold recently shot video of
patients and caregivers in Liberia and Sierra Leone, at the epicenter of
the Ebola outbreak that has killed more than 4,400 people in recent months. Now
home in Washington, D.C., Brandvold is spending 21 days in self-imposed
isolation... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/10/experiences-from-ebola-outbreak-zone.html

TWITTER, FACEBOOK AND ZINCH MAY HELP YOU TO FIND OUT SCHOLARSHIPS

🕐 October 12, 2014

Going to college can be expensive, especially after the four or five years it
takes you to finish your undergraduate degree. The average American student
graduates from college owing $20,000 in debt. However, there are ways to make
your college experience less of a financial burden, and applying for
scholarships is a fantastic way to do so. Not only will you receive financial
rewards, but winning a scholarship can also help you gain stable employment
after graduation or help you out if you're look... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/10/twitter-facebook-and-zinch-may-help-you.html

THE NOBEL PRIZE WINNERS IN PHYSIOLOGY OR MEDICINE 2014

🕐 October 07, 2014

The Nobel Prize in Physiology or Medicine 2014 Photo: David Bishop, UCL John
O'Keefe Prize share: 1/2 Photo: Kavli Institute, NTNU, CC-BY-SA-3.0 via
Wikimedia Commons May-Britt Moser Prize share: 1/4 Photo: Kavli Institute, NTNU,
CC-BY-SA-3.0 via Wikimedia Commons Edvard I. Moser Prize share: 1/4 The Nobel
Prize in Physiology or Medicine 2014 was divided, one half awarded to John
O'Keefe, the other half jointly to May-Britt Moser and Edvard I. Moser&nb...
Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/10/the-nobel-prize-winners-in-physiology.html

CAREER OPPORTUNITIES IN MEDICAL BIOCHEMISTRY DEGREE

🕐 September 26, 2014

Biochemistry involves studying the chemistry of living things such as
substances, compounds and processes. Biochemists combine the fields of
microbiology, cell biology, genetics, chemistry, cell biology, and physics in
their day-to-day work or experiments. They get into the most, minute
characteristics of organisms, and their biological processes. They may be
identifying the way in which DNA, which carries the genetic information, is
transferred between cells and can be manipulated. Biochemistry... Read More
Visit
http://edusanjalbiochemist.blogspot.com/2014/09/career-opportunities-in-medical.html

BREAST CANCER: INTRODUCTION, PATHOPHYSIOLOGY AND DIAGNOSIS

🕐 September 18, 2014

Breast Cancer: Where It Starts The earlier a breast cancer lump is found,
identified, and treated, the better the outlook. That’s why breast cancer
testing plays such a key role in so many facets of the disease. Unfortunately,
signs of breast cancer are rarely visible from the surface of the skin. Breast
self-exams should be done regularly to detect physically substantial lumps under
the skin. But even self-exams may miss some cases. By moving the slide from left
to right, you will see where ... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/09/breast-cancer-introduction.html

THE THREE MAJOR STRESS HORMONES: ADRENALINE, CORTISOL, NOREPINEPHRINE

🕐 September 09, 2014

Thanks to the work of our sympathetic nervous system, the "fight or flight"
system that takes over when we're stressed, when you see your boss's name in
your inbox late at night, your body reacts like there's a lion on the loose.
Behind the wide range of both physical and mental reactions to stress are a
number of hormones that are in charge of adding fuel to the fire. Adrenaline
What It Is: Commonly known as the fight or flight hormone, it is produced by the
adrenal glands after re... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/09/the-three-major-stress-hormones.html

DIABETES COULD BE CURED: A STUDY FROM SCIENTISTS AT MANCHESTER UNIVERSITY

🕐 August 21, 2014

(adsbygoogle = window.adsbygoogle || []).push({}); Scientists at Manchester
University have discovered that the hormone amylin can clump together and stop
cells producing insulin Fig. Diabetes could be cured with medication after
scientists discovered that clumps of amylin may be stopping cells producing
insulin (Photo courtesy: Alamy) Diabetes could be cured after scientists
discovered that toxic clumps of a hormone stop cells producing insulin.
Scientists at Manchester University have fo... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/08/diabetes-could-be-cured-study-from.html

EBOLA VIRUS OUTBREAK IN WEST AFRICA AND ITS RISK ASSESSMENT

🕐 August 10, 2014

(adsbygoogle = window.adsbygoogle || []).push({}); The current Ebola Virus
outbreak began in Guinea in December 2013. This outbreak now involves
transmission in Guinea, Liberia, Nigeria, and Sierra Leone. As of 4 August 2014,
countries have reported 1 711 cases (1 070 confirmed, 436 probable, 205
suspect), including 932 deaths. This is currently the largest EVD outbreak ever
recorded. And on 8 August 2014, WHO declared the Ebola outbreak in West Africa a
Public Health Emergency of International Co... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/08/ebola-virus-outbreak-in-west-africa-and.html

PREANALYTICAL VARIABLES : CONTROLLABLE OR UNCONTROLLABLE (PART 3)

🕐 August 05, 2014

Standardization of specimen collection practices help reduce errors due to
preanalytical variables. Major physiological variables include posture,
prolonged bed rest, exercise, physical training, circadian variation and travel.
Other physiological variables include diet, lifestyle, stimulants, drugs, herbal
preparations, recreational drug ingestions etc. Controllable Parameters Posture
– Changes water and plasma protein distribution. Many hormones’ values are
affected, some of them dras... Read More Visit
http://edusanjalbiochemist.blogspot.com/2014/08/preanalytical-variables-controllable-or.html
Biochemistry Class notes
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