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Search job by email or one or more job IDs, job IDs should be separated by
commas.

 * Home
 * WGBS
 * RRBS
 * DMR
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Welcome to WBSA
This is a free web service for analysis of Whole-Genome Bisulfite-Sequencing
(WGBS) and Genome-wide Reduced Representation Bisulfite Sequencing (RRBS) data.
WBSA not only focuses on CpG methylation, but also allows CHG and CHH analysis.
BWA is incorporated as its mapping software. WBSA can be applied to DNA
methylation researches for animals and plants and it provides advanced analysis
for both WGBS and RRBS. It can also identify differently methylated regions
(DMRs) in different strings. WBSA includes six modules: Home, WGBS, RRBS, DMR,
Documents and Downloads, and provides the executable package for downloads and
local installation.
WGBS and RRBS modules include four main steps: pre-processing of reads and
reference, alignment to the reference, identification of methylcytosines and
annotation. DMR module includes DMRs identification and annotation of the
correlative genes.
A concise workflow is shown in the following charts:

In this web service, users can upload the NGS bisulfite sequencing data, fill in
some necessary parameters, and then click the "Submit" button to create a task.
WBSA can handle user's request and execute the task in preset server, which is a
powerful linux cluster server. While beginning and finishing the task, an
auto-email will be sent to user if the email address is available, then users
can check uploaded parameters, monitor the task or view the results.
WBSA provides a simple data processing method (pipeline) and the free
computational resources for users. It will be helpful for the biologists and
bioinformaticians who are working on DNA methylation.
WBSA is available now for both online and offline. Features for researchers
include:
    ♦  Easy to use web-based application
    ♦  Short reads mapping and result report
    ♦  Sequence annotation based on UCSC, TIGR, et al.
    ♦  Data mining on sequence alignments and text annotations
    ♦  Analysis and annotation of methylation level, mC distribution, and ratio
et al. (including CG and non-CG)
    ♦  Correlation analysis between methylation level and gene functional
regions, repeat regions, CpG islands, and DMRs( different methylation regions)
et al.
Contact us
WBSA is developed and maintained by Beijing Institute of Genomics(BIG),Chinese
Academy of Sciences. The network server and related software are only free of
charge for non-commercial users. If you have any feedback or question concerning
the tools or data on this website, please feel free to contact us at
wbsa@big.ac.cn.
What's new in WBSA?
  Fix the algorithm for methylation level statistics.(2013-05-27)
  The FTP module is added to this site to solve big data
transferring.(2013-05-10)
  The executable package can be downloaded from this site freely.(2013-03-22 )
  The DMR module launched. (2013-02-15)
  Genomes of 10 species and its transformed sequences are integrated in this
pipeline. (2012-12-20)
  The WGBS and RRBS modules are launched. (2012-12-20)
  WBSA is released to public, user can use it freely. (2012-12-20)
References
Highly integrated single-base resolution maps of the epigenome in
Arabidopsis.[Cell. 2008]
Human DNA methylomes at base resolution show widespread epigenomic
differences.[Nature. 2009]
Increased methylation variation in epigenetic domains across cancer types. [Nat
Genet. 2011]
Fast and accurate short read alignment with Burrows-Wheeler
transform.[Bioinformatics. 2009]
Reduced representation bisulfite sequencing for comparative high-resolution DNA
methylation analysis.[Nucleic Acids Res. 2005]
Comparison of alignment software for genome-wide bisulphite sequence
data.[Nucleic Acids Res. 2012]
Other BIG tools
   GOBAND: Scaffolding Program
   MeRIP-PF: MeRIP-Seq Peak-finding Pipeline
   wapRNA: RNA-seq Analysis Pipeline
Useful links
   UCSC
   Bwa
   WEBLOGO



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