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* Alpha
ALPHA ASSAYS
View products & services View resource library
Virtually any target can be quickly and easily measured using Alpha technology,
an improved alternative to quantitative ELISA assays and ELISA reagents. Such
targets include enzymes, receptor-ligand interactions, low-affinity
interactions, second messenger levels, DNA, RNA, proteins, protein-protein
interactions, peptides, sugars and small molecules.
* Ease-of-use: homogeneous assay with no wash requirement and no separation
steps
* Fast: results in 3 hours for most applications
* High sensitivity: detect down to femtogram levels of target
* Large dynamic range: 4-5 logs
* Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF),
tissue extracts, cell culture supernatants, cell lysates, and more
Choose the homogenous assay right for you:
* AlphaLISA™ – when running large numbers of ELISA assays from the same sample
or working with a difficult matrix, serum or plasma
* AlphaLISA SureFire® Ultra™ – an excellent choice for studying pathways or
phosphorylated proteins in whole cells, or studying endogenous receptors
* AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein
interactions, and for testing cAMP, phosphorylated peptides or samples in a
simple matrix
* Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative
ELISA assay, the toolbox gives you everything you need to create your own
For research use only. Not for use in diagnostic procedures.
Shop now
BIOMARKER/CYTOKINE DETECTION
Detection and quantitation of molecular targets in serum, plasma, culture
supernatants, cellular lysates, and other sample types.
Detection and quantitation of molecular targets in serum, plasma, culture
supernatants, cellular lysates, and other sample types.
Convert troublesome traditional ELISAs to a far superior alternative - Revvity's
highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines.
Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy
automation for analysis of biomarkers, cytokines and other analytes. With
AlphaLISA you can also shorten the time it takes you to run assays to less than
3 hours and benefit from small sample volumes (1-5 µL) to conserve precious
sample.
CELL SIGNALING
AlphaLISA SureFire Ultra and Alpha SureFire Multiplex assay kits to measure
protein phosphorylation in cell signaling pathways.
AlphaLISA SureFire Ultra and Alpha SureFire Multiplex assay kits to measure
protein phosphorylation in cell signaling pathways.
* All-in-one-well alternative to Western Blotting
* Flexibility to use cultured cell lines, stem cells and primary cells
* Cost-effective and flexible – a single plate can accommodate any assay for
any target
* Great sensitivity and dynamic range
* Easy automation
* No harmful chemicals and non-radioactive, eliminating special reagent
handling and disposal
PROTEIN-PROTEIN INTERACTIONS
Handle large biomolecular interactions with ease.
Handle large biomolecular interactions with ease.
* Ease-of-use: few addition steps, fast assay development
* Broad range of affinities: detect strong or weak interactions, from pM to mM
affinity
* Distance: measure very large protein or antibody complexes – spanning up to
200 nm or more
* High avidity: multiple binding sites on each bead enables use of nanomolar
concentrations of antibodies or proteins, as well as use of low affinity
binders
* Conserve reagents: robust assays using nM concentrations of
proteins/antibodies
EPIGENETICS
Epigenetics is the analysis of heritable traits that are not transmitted by
sequence information but rather through modification of the DNA methylation
state and Histone methylation/acetylation driven by environmental factors such
as lifestyle, toxins and stress.
Epigenetics is the analysis of heritable traits that are not transmitted by
sequence information but rather through modification of the DNA methylation
state and Histone methylation/acetylation driven by environmental factors such
as lifestyle, toxins and stress.
Revvity made available for epigenetics research a range of biochemical and
cellular assays. AlphaLISA® epigenetics toolbox reagents were designed for the
detection of specific methylation and acetylation marks on histone peptide
substrates. AlphaLISA epigenetic cellular detection kits were designed to detect
endogenous epigenetic modifications on Histone 3 in a no-wash, all-in-one well
format. In addition to the acetylation and methylation assays mentioned above,
other types of post-translational modification detection assays have been
created using Alpha reagents to detect specific epigenetic marks on both peptide
and full-length histone and protein substrates.
GPCR RESEARCH
Measure cAMP and downstream phosphorylation of proteins for functional GPCR
assays.
Measure cAMP and downstream phosphorylation of proteins for functional GPCR
assays.
FEATURED RESOURCES
APPLICATION NOTE
Detection of MAPK activation to evaluate the efficacy and potency of KRAS-SOS1
inhibitors by AlphaLISA and HTRF technologies
Learn more
EBOOK
Autophagy regulation eBook
Learn more
LITERATURE - PUBLICATION REVIEW
Novel drugs for cancer therapy development with PROTAC
Learn more
APPLICATION NOTE
Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures
in a Prostate Cancer
Learn more
APPLICATION NOTE
Developing novel tetraubiquitin substrates and AlphaLISA technology to provide
next generation deubiquitinase assays
Learn more
APPLICATION NOTE
cAMP assay provides flexibility and stable pharmacology
Learn more
PUBLICATION REVIEW
Key signaling pathways in liquid cancer: therapeutic targets, screening tools,
and future developments
Learn more
PUBLICATION REVIEW
HTRF and AlphaLISA assays bring clarity to extracellular matrix related fibrosis
development
Learn more
View all resources
Products & Services
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1 - 25 OF 1698 PRODUCTS AND SERVICES
New
AlphaLISA Human Neurofilament L (NF-L) Detection Kit
The AlphaLISA™ Human Neurofilament Light (NF-L) Kit is designed for the simple
and rapid quantification of human NF-L in cell supernatants.
Part Number: AL3199HV, AL3199C, AL3199F
EUR 949.00 - 13,848.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Human and Mouse Phospho-Rb (Thr821) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-Rb (Thr821) assay is a
sandwich immunoassay for quantitative detection of phospho-Rb in cellular
lysates using Alpha Technology.
Part Number: ALSU-PRB-D-HV, ALSU-PRB-D500, ALSU-PRB-D10K, ALSU-PRB-D50K
EUR 751.00 - 49,943.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Mouse Total RIPK1 Detection Kit
The AlphaLISA™ SureFire® Ultra™ Mouse Total RIPK1 assay is a sandwich
immunoassay for quantitative detection of total RIPK1 in cellular lysates using
Alpha Technology.
Part Number: ALSU-TRIPK1-B-HV, ALSU-TRIPK1-B500, ALSU-TRIPK1-B10K,
ALSU-TRIPK1-B50K
EUR 751.00 - 49,943.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Human and Mouse Phospho-PRAS40 (Thr246) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-PRAS40 (Thr246) assay is
a sandwich immunoassay for quantitative detection of phospho-PRAS40 in cellular
lysates using Alpha Technology.
Part Number: ALSU-PPRAS-A-HV, ALSU-PPRAS-A500, ALSU-PPRAS-A10K, ALSU-PPRAS-A50K
EUR 751.00 - 49,943.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Human Phospho-eNOS (Ser1177) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human Phospho-eNOS (Ser1177) assay is a sandwich
immunoassay for quantitative detection of phospho-eNOS in cellular lysates using
Alpha Technology.
Part Number: ALSU-PENOS-A-HV, ALSU-PENOS-A500, ALSU-PENOS-A10K, ALSU-PENOS-A50K
EUR 751.00 - 49,943.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Human Total p16 INK4A Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human Total p16 INK4A assay is a sandwich
immunoassay for quantitative detection of total p16 INK4A in cellular lysates
using Alpha Technology.
Part Number: ALSU-TP16INK4-A-HV, ALSU-TP16INK4-A500, ALSU-TP16INK4-A10K,
ALSU-TP16INK4-A50K
EUR 751.00 - 49,943.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Human Phospho-PERK (Thr982) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human Phospho-PERK (Thr982) assay is a sandwich
immunoassay for quantitative detection of phospho-PERK in cellular lysates using
Alpha Technology.
Part Number: ALSU-PPERK-B-HV, ALSU-PPERK-B500, ALSU-PPERK-B10K, ALSU-PPERK-B50K
EUR 751.00 - 49,943.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Human Total Progesterone Receptor Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human Total Progesterone Receptor assay is a
sandwich immunoassay for quantitative detection of total Progesterone Receptor
in cellular lysates using Alpha Technology.
Part Number: ALSU-TPROGR-A-HV, ALSU-TPROGR-A500, ALSU-TPROGR-A10K,
ALSU-TPROGR-A50K
EUR 751.00 - 49,943.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Human Total PI3K p85 Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human Total PI3K p85 assay is a sandwich
immunoassay for quantitative detection of total PI3K p85 in cellular lysates
using Alpha Technology.
Part Number: ALSU-TPI3K-B-HV, ALSU-TPI3K-B500, ALSU-TPI3K-B10K, ALSU-TPI3K-B50K
EUR 751.00 - 49,943.00
View Details
Add to cart
New
AlphaLISA Hepatitis B Virus e Antigen (HBeAg) Detection Kit
The AlphaLISA™ Detection Kit for Hepatitis B Virus Surface antigen (HBeAg)
enables the quantitative determination of HBeAg in cell culture media and buffer
using a homogeneous AlphaLISA assay (no wash steps).
Part Number: AL3201HV, AL3201C, AL3201F
EUR 1,213.00 - 23,956.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Mouse Phospho-RIPK1 (Ser321) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Mouse Phospho-RIPK1 (Ser321) assay is a sandwich
immunoassay for quantitative detection of phospho-RIPK1 in cellular lysates
using Alpha Technology.
Part Number: ALSU-PRIPK1-C-HV, ALSU-PRIPK1-C500, ALSU-PRIPK1-C10K,
ALSU-PRIPK1-C50K
EUR 751.00 - 49,943.00
View Details
Add to cart
New
AlphaLISA SureFire Ultra Human and Mouse Total MEK2 Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total MEK2 assay is a sandwich
immunoassay for quantitative detection of total MEK2 in cellular lysates using
Alpha Technology.
Part Number: ALSU-TMEK2-A-HV, ALSU-TMEK2-A500, ALSU-TMEK2-A10K, ALSU-TMEK2-A50K
EUR 751.00 - 49,943.00
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New
AlphaLISA FCGR1/CD64 Binding Kit
This kit is designed for the detection of binding between FCGR1 (CD64) and human
IgG Fc fragment using a homogeneous AlphaLISA™ assay (no wash steps). This assay
can facilitate the design and development of antibody therapetics by using
competitive binding.
Part Number: AL3200C, AL3200F
EUR 3,434.00 - 17,141.00
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New
AlphaLISA SureFire Ultra Human Phospho-PI3K p85 (Tyr467) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human Phospho-PI3K p85 (Tyr467) assay is a
sandwich immunoassay for quantitative detection of phospho-PI3K p85 in cellular
lysates using Alpha Technology.
Part Number: ALSU-PPI3K-B-HV, ALSU-PPI3K-B500, ALSU-PPI3K-B10K, ALSU-PPI3K-B50K
EUR 751.00 - 49,943.00
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AlphaLISA SureFire Ultra Human and Mouse Total NFκB2 p100 Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total NFκB2 p100 assay is a
sandwich immunoassay for quantitative detection of total NFκB2 p100 in cellular
lysates using Alpha Technology.
Part Number: ALSU-TNFKB2-B-HV, ALSU-TNFKB2-B500, ALSU-TNFKB2-B10K,
ALSU-TNFKB2-B50K
EUR 751.00 - 49,943.00
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AlphaLISA SureFire Ultra Human and Mouse Phospho-MEK2 (Ser217/221) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-MEK2 (Ser217/221) assay
is a sandwich immunoassay for quantitative detection of phospho-MEK2 in cellular
lysates using Alpha Technology.
Part Number: ALSU-PMEK2-A-HV, ALSU-PMEK2-A500, ALSU-PMEK2-A10K, ALSU-PMEK2-A50K
EUR 751.00 - 49,943.00
View Details
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New
AlphaLISA SureFire Ultra Human Phospho-RIPK1 (Ser320) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human Phospho-RIPK1 (Ser320) assay is a sandwich
immunoassay for quantitative detection of phospho-RIPK1 in cellular lysates
using Alpha Technology.
Part Number: ALSU-PRIPK1-B-HV, ALSU-PRIPK1-B500, ALSU-PRIPK1-B10K,
ALSU-PRIPK1-B50K
EUR 751.00 - 49,943.00
View Details
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New
AlphaLISA SureFire Ultra Human and Mouse Total NFκB2 p100/p52 Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total NFκB2 p100/p52 assay is a
sandwich immunoassay for quantitative detection of total NFκB2 p100/p52 in
cellular lysates using Alpha Technology.
Part Number: ALSU-TNFKB2-A-HV, ALSU-TNFKB2-A500, ALSU-TNFKB2-A10K,
ALSU-TNFKB2-A50K
EUR 751.00 - 49,943.00
View Details
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New
AlphaLISA SureFire Ultra Human and Mouse Total PRAS40 Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total PRAS40 assay is a sandwich
immunoassay for quantitative detection of total PRAS40 in cellular lysates using
Alpha Technology.
Part Number: ALSU-TPRAS-A-HV, ALSU-TPRAS-A500, ALSU-TPRAS-A10K, ALSU-TPRAS-A50K
EUR 751.00 - 49,943.00
View Details
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New
AlphaLISA SureFire Ultra Human Phospho-eNOS (Thr495) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human Phospho-eNOS (Thr495) assay is a sandwich
immunoassay for quantitative detection of phospho-eNOS in cellular lysates using
Alpha Technology.
Part Number: ALSU-PENOS-B-HV, ALSU-PENOS-B500, ALSU-PENOS-B10K, ALSU-PENOS-B50K
EUR 751.00 - 49,943.00
View Details
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New
AlphaLISA SureFire Ultra Human and Mouse Total TRAF3 Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total TRAF3 assay is a sandwich
immunoassay for quantitative detection of total TRAF3 in cellular lysates using
Alpha Technology.
Part Number: ALSU-TTRAF3-A-HV, ALSU-TTRAF3-A500, ALSU-TTRAF3-A10K,
ALSU-TTRAF3-A50K
EUR 751.00 - 49,943.00
View Details
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New
AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human Total p21 CiP1 assay is a sandwich
immunoassay for quantitative detection of total p21 CiP1 in cellular lysates
using Alpha Technology.
Part Number: ALSU-TP21CIP-A-HV, ALSU-TP21CIP-A500, ALSU-TP21CIP-A10K,
ALSU-TP21CIP-A50K
EUR 751.00 - 49,943.00
View Details
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New
AlphaLISA SureFire Ultra Human and Mouse Phospho-Rb (Thr826) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-Rb (Thr826) assay is a
sandwich immunoassay for quantitative detection of phospho-Rb in cellular
lysates using Alpha Technology.
Part Number: ALSU-PRB-E-HV, ALSU-PRB-E500, ALSU-PRB-E10K, ALSU-PRB-E50K
EUR 751.00 - 49,943.00
View Details
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New
AlphaLISA SureFire Ultra Human and Mouse Total BAD Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total BAD assay is a sandwich
immunoassay for quantitative detection of total BAD in cellular lysates using
Alpha Technology.
Part Number: ALSU-TBAD-A-HV, ALSU-TBAD-A500, ALSU-TBAD-A10K, ALSU-TBAD-A50K
EUR 751.00 - 49,943.00
View Details
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New
AlphaLISA SureFire Ultra Human and Mouse Phospho-BAD (Ser136) Detection Kit
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-BAD (Ser136) assay is a
sandwich immunoassay for quantitative detection of phospho-BAD in cellular
lysates using Alpha Technology.
Part Number: ALSU-PBAD-B-HV, ALSU-PBAD-B500, ALSU-PBAD-B10K, ALSU-PBAD-B50K
EUR 751.00 - 49,943.00
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* Technical Note (43)
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1 - 25 OF 152 RESOURCES
Application Note
A COMPARATIVE STUDY OF TWO IMMUNOASSAY PLATFORMS TO DETERMINE LENTIVIRUS TITER
FOR CAR-T DEVELOPMENT
The main vectors of gene therapy in research are viruses. The most popular tool
for gene delivery is a genetically modified lentivirus. Modified lentivirus
(HIV-1) vectors retain their ability to infect undivided cells, thereby
increasing their ability to transduce a wide variety of cells, including those
that are difficult to transduce. This advantage enables the stable long-term
expression of a transgene. In immunotherapy, CAR-T cells are manufactured by
transducing the CAR gene with an HIV-1 vector in T cells to express a specific
chimeric p24 protein on their surface. This allows them to recognize cancer
cells and destroy them. These CAR-T cells must be generated individually to
treat each patient. This application note demonstrates a comparative
quantification of the p24 titer in a lentiviral GFP control sample using
Alliance HIV-1 p24 Antigen ELISA and p24 AlphaLISA immunoassay platforms. Check
out the different sections of this application note: A lentiviral vector that
encodes the CAR construct Determination of the efficiency of transient
co-transfection by measuring viral titer Detection of the presence of a p24
HIV-1 specific antibody in the sample Quantification of targets present in the
sample
Learn More
Application Note
A FAST AND SIMPLE CHEMILUMINESCENT ASSAY FOR MONITORING OF DNA-PROTEIN
INTERACTIONS
Electrophoretic Mobility Shift Assay (EMSA) is a standard technique used to
study protein-DNA interactions. It is a radioactive assay, suitable only for
low-throughput applications due to the need for a gel-based separation step.
AlphaLISA ™ is a bead-based chemiluminescent technology, as a faster, simpler,
and non-radioactive alternative to EMSA. AlphaLISA ™ is highly sensitive,
requiring less nuclear extract compared to EMSA, highly reproducible, and
suitable for higher throughput applications. Revvity developed an AlphaLISA
immunoassay to monitor the presence of specific DNA-binding proteins in nuclear
extracts. In this application note, we use HepG2 nuclear extracts to demonstrate
the binding of Sp1 and HNF1 transcription factors to tagged oligonucleotides
containing required cognate response elements. Read this note to see the results
and access detailed data compared to EMSA.
Learn More
Application Note
AAV CAPSID DETECTION AND CHARACTERIZATION WITH NEW ALPHALISA ASSAY KITS
Reliable, Quantitative, and No-Wash Assay Platform for AAV Capsid Detection AAVs
are widely used as viral vectors in human gene therapy implementing recombinant
DNA technology. Measurement of AAV concentration in complex biological matrices
is essential for and effective manufacturing of AAV gene therapies. Revvity has
developed and manufactured a line of immunoassays that detect and quantify AAV
capsid with AlphaLISA™ technology. These assays can detect AAV1, AAV2, AAV3B,
AAV5, AAV6, AAV8, and AAV9 serotypes, and measure AAV particles present in cell
culture media, lysis buffer, and cell lysate. Get your application note to:
Learn more about AAV capsid detection kits Access data on detection limit, assay
reproducibility, variability, specificity, and recovery from multiple analyte
matrices through explained diagrams See details on experimental conditions
Learn More
Whitepaper
ADDRESSING THE CHALLENGES OF BISPECIFIC ANTIBODY CHARACTERIZATION WITH
HIGH-THROUGHPUT PLATFORMS
Bispecific antibodies (bsAbs) have emerged as an attractive class of therapeutic
agents with the potential to revolutionize the treatment of various disorders.
While the concept of bsAbs has been around for several decades, it has taken
time for the technology and understanding of antibody engineering to advance to
a point where its practical application has become feasible. The development of
bsAbs demands intricate screening processes to identify candidates with desired
binding properties, ensuring they induce specific biological activities.
Additionally, achieving optimal tissue tropism and potency requires careful
consideration of factors such as antibody size, affinity, and the implementation
of innovative characterization strategies. In this context, this whitepaper
explores novel approaches contributing to bsAb characterization, offering
real-world examples that highlight their practical applications.
Learn More
Whitepaper
ADENO-ASSOCIATED VIRUS (AAV) VECTORS MANUFACTURING
What Are the Challenges and Solutions? All across the globe, AAVs are getting
the attention of scientists and companies working in gene therapy, as they
provide the right combination of characteristics that make them one of the most
promising vector today. In 2021 only, the global gene therapy market was 4.1
billion USD, and AAV vector therapies made up more than 43% of that market
value! Designing and manufacturing AAV vectors is complex, and to be successful,
certain challenges must be addressed. This implies monitoring and optimtizing
production for a thorough quality control process, including reliable ongoing
characterization of process intermediates and the final product. Key features
include: Overview and data on the gene therapy market Description of AAV
vectors’ genomes and transfer Challenges associated with AAV vectors’ design and
manufacturing Analytical methods for AAV vector quality control with a
description of innovative no-wash AlphaLISA™ assays
Learn More
Flyer
ADVANCE YOUR BIOLOGICS RESEARCH WITH REVVITY SOLUTIONS
In the dynamic landscape of pharmaceuticals, biotherapeutics research stands out
as the fastest-growing sector. Scientists worldwide are on a quest for ever more
efficient tools to develop therapeutic proteins. From lead selection to
bioprocessing qualification, rigorous analytical characterization is essential.
That’s where Revvity steps in. Our comprehensive line of no-wash tools
revolutionizes biologics screening, mechanism-of-action studies, and
biomanufacturing. With robustness and convenience at the forefront, we empower
researchers to accelerate discoveries and drive innovation. Welcome to a new era
of precision and efficiency in biotherapeutics research. For research use only.
Not for use in diagnostic procedures.
Learn More
Application Note
ADVANTAGES OF USING OUR AUTOMATABLE AND NO-WASH HTRF AND ALPHALISA KITS FOR CHO
HCP DETECTION
Detecting and quantifying HCPs with automatable homogeneous immunoassays During
biotherapeutic manufacturing and production, the host cells - a great majority
of them being Chinese Hamster Ovary (CHO) cells – produce protein impurities
that are called Host Cell Proteins (HCPs). Even if more than 99% of them are
removed from the final product, the residual CHO HCPs can induce immunogenicity
in individuals or reduce the potency, stability, or effectiveness of a drug.
Therefore, to meet regulatory organizations’ guidelines (such as FDA or EMA) on
CHO HCP levels, biopharmaceutical companies spend significant amounts of money
on tools and strategies for their detection. Illustrated with robust results,
this Application Note explains the many ways in which HTRF™ and AlphaLISA™ kits
can improve the workflow for CHO HCP detection: Wide antibody coverage
Compatibility with most commonly used buffers No cross-reactivity between CHO
HCP detection and drug substance Good dilutional linearity and antigen spike
recovery
Learn More
Brochure
ALPHA ASSAYS AND REAGENTS CATALOG
Alpha technolgy enables the rapid and straightforward mesaure of virtually any
target. This includes enzymes, receptor-ligand interactions, low-affinity
interactions, second messenger levels, DNA, RNA, proteins, protein-protein
interactions, peptides, sugars and small molecules. Explore the vast selection
of homogenous Alpha assays and reagents, including AlphaLISA ™ , AlphaLISA ™
SureFire ® Ultra ™ , AlphaScreen ™ , and Alpha Toolbox. For research use only.
Not for use in diagnostic procedures.
Learn More
Guide
ALPHA PROTEIN-PROTEIN INTERACTION QUICK START GUIDE
Alpha has been used to study a wide variety of interactions, including
protein:protein, protein:peptide, protein:DNA, protein:RNA,
protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear
receptor:ligand interactions. Both cell-based and biochemical interactions have
been monitored, and applications such as phage display, ELISA, and EMSA
(electrophoretic mobility shift assay) have been adapted to Alpha.
Learn More
Brochure
ALPHA SUREFIRE ULTRA NO-WASH IMMUNOASSAY CATALOG
Discover Alpha SureFire ® Ultra ™ assays, the no-wash cellular kinase assays
leveraging Revvity's exclusive bead-based technology and sandwich immunoassays
for detecting phosphorylated proteins in cells. Offering a quantitative
alternative to Western Blotting, Alpha SureFire assays are automation-friendly,
easily miniaturized, and proficient in detecting both endogenous and recombinant
proteins. Explore our comprehensive portfolio of SureFire Assays, designed to
help you elevate and expedite your drug discovery journey.
Learn More
Whitepaper
ALPHA TECHNOLOGIES FOR ANTIBODY DETECTION AND CHARACTERIZATION
Many laboratories developing and producing antibodies still rely on traditional
enzyme-linked immunosorbent assay (ELISA) to perform clonal selection and
characterization. Whilst well established, ELISAs can lack sensitivity and
reproducibility, and are difficult to automate. In this white paper, you will
learn how Alpha Technologies can provide a faster and simpler workflow for
antibody detection and characterization. It will take you through key steps such
as antibody clonal selection, measuring antibody affinity and characterizing
antibodies, providing examples and demonstrating the advantages Alpha technology
can provide as an alternative to ELISA. Download the white paper to discover how
to advance your antibody detection and characterization.
Learn More
Application Note
ALPHA TECHNOLOGY: A FAST AND SENSITIVE ORTHOGONAL APPROACH TO CELL-BASED POTENCY
ASSAYS
Orthogonal systems to cell-based assays are a key requirement in EMA/FDA
guidelines for potency estimations and require cross-validation with
complementary approaches to prove and strengthen the reliability of results. In
this application note published in collaboration with IBR Inc., you will learn:
Why Alpha technology represents an ideal cell-free orthogonal system for potency
assays How AlphaLISA assays can be used to determine Bevacizumab/VEGF165 potency
An example of how to run an AlphaLISA potency assay and the type of data that
can be generated Why it is suitable for assessing lot-to-lot consistency and
equivalence of Bevacizumab and biosimilars
Learn More
Application Note
ALPHA TERBIUM SUREFIRE ULTRA MULTIPLEX: SIMULTANEOUS DUAL PHOSPHOPROTEIN TARGET
ANALYSIS
Evaluating pharmaceutical compound efficacy and safety in regulating cell
behavior can involve the study of multiple signal transduction pathways and
measuring more than one cellular target can provide greater confidence of
positive compound hits on the cellular target of interest. Protein
phosphorylation has been identified as a useful readout of cellular activation
or inhibition, and these pathways are commonly targeted for therapeutic
modulation of disease. Numerous assay technologies are available to look at
protein phosphorylation for drug development, including the AlphaLISA ™ SureFire
® Ultra which provides a highly sensitive, fully homogenous option for the
analysis of signaling pathways. This application note details the implementation
of multiplexing with the AlphaLISA SureFire Ultra Multiplex assay, which offers
a homogenous and high throughput approach to multiplexing.
Learn More
Technical Note
ALPHALISA ACETYL‑HISTONE H3 LYSINE 27 (H3K27AC) CELLULAR DETECTION KIT
Quantifying H3K27ac Levels with AlphaLISA Assay In this technical note, you will
discover how AlphaLISA immunodetection assay easily detects alterations in
acetylated histone H3 lysine 27 (H3K27ac) levels within cellular extracts.
Learn More
Technical Note
ALPHALISA ACETYL‑HISTONE H3 LYSINE 9 (H3K9AC) CELLULAR ASSAY
Quantifying H3K9ac in Cellular Extracts with AlphaLISA This technical note gives
you details about how AlphaLISA immunodetection assay monitors changes in the
levels of acetylated histone H3 lysine 9 (H3K9ac) in cellular extracts.
Learn More
Guide
ALPHALISA ASSAY DEVELOPMENT GUIDE
The AlphaLISA technology allows the detection of molecules of interest in serum,
plasma, cell culture supernatants or cell lysates in a very sensitive,
quantitative, reproducible, and user-friendly assay. Almost, any sandwich assay
can be developed to detect an analyte of interest. Discover how to simply your
immunoassay workflow with this guide! For research use only. Not for use in
diagnostic procedures.
Learn More
Technical Note
ALPHALISA ASSAYS FOR IMMUNE CHECKPOINT DETECTION AND QUANTITATION
Quantifying Immune Checkpoints: AlphaLISA Biomarker Detection Kits This
technical note focuses on the role of checkpoint molecules in cancer
immunotherapies. Checkpoint molecules are molecular markers that can regulate an
immune system attack on cancer cells. They have gained prominence as promising
candidates for immunotherapies. AlphaLISA detection kits are designed to detect
and quantify the levels of these molecules in cell culture media, serum, and
cell lysates.
Learn More
Technical Note
ALPHALISA DI-METHYL-HISTONE H3 LYSINE 4 (H3K4ME2) CELLULAR ASSAY
Decoding Histone Modifications: A Targeted Assay for H3K4me2 In this study, you
will find details about an optimized assay designed to quantify changes in
H3K4me2 levels following treatment with sodium butyrate and Trichostatin A
(TSA), both of which are non-selective histone deacetylase (HDAC) inhibitors.
Using a standardized histone extraction procedure, we detect the target mark by
employing a biotinylated anti-Histone H3 (C-terminus) antibody and AlphaLISA
Acceptor beads conjugated to an antibody specific to the mark.
Learn More
Technical Note
ALPHALISA DI/MONO-METHYL-HISTONE H3 LYSINE 27 (H3K27ME2-1) CELLULAR DETECTION
KIT
Quantification of H3K27me3 Levels Using AlphaLISA Technology In this technical
note discover how AlphaLISA ™ immunodetection assay tracks variations in
cellular extract levels of di-methylated histone H3 lysine 27 (H3K27me2-1).
Learn More
Technical Note
ALPHALISA DOT1L HISTONE H3 LYSINE-N-METHYLTRANSFERASE ASSAY
Unlocking the secrets of epigenetics, our technical not dives into optimizing a
DOT1L enzymatic assay This technical document outlines the optimization of a
DOT1L enzymatic assay using oligonucleosomes as the substrate. Discover how the
assay easily detects the dimethylated product of histone H3 lysine 79 by
leveraging a biotinylated anti-H3K79me2 antibody, which binds to the epigenetic
mark of interest.
Learn More
Technical Note
ALPHALISA EZH2 HISTONE H3-LYSINE 27 N-METHYLTRANSFERASE ASSAY
Lysine 27 Dimethylation Detection with AlphaLISA Technology In this technical
note discover how AlphaLISA immunodetection assay detects dimethylation of a
biotinylated Histone H3 (21-44) peptide at lysine 27. It uses
anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) as the detection antibody.
Learn More
Technical Note
ALPHALISA FOR BIOMARKERS IN URINE: MEASURING THE RENAL TUBULAR INJURY INDICATOR,
Β2-MICROGLOBULIN
AlphaLISA ™ Detects β2-Microglobulin in Urine with High Sensitivity When kidney
tubular function is impaired, elevated levels of β2-microglobulin in urine
indicate renal filtration or reabsorption disorders. Measuring urinary
β2-microglobulin can help assess tubular function, but variability in urine
composition may affect results. In this study, AlphaLISA ™ technology is
demonstrated for the first time in the complex matrix of urine, and is shown to
detect the renal tubular injury marker Beta2-microglobulin (β2-microglobulin),
in urine at a sensitivity of 77 pg/mL.
Learn More
Technical Note
ALPHALISA G9A HISTONE H3-LYSINE N-METHYLTRANSFERASE ASSAY
Quantification of Histone H3 Lysine 9 Di-Methylation Using AlphaLISA Assay In
this technical note discover how The AlphaLISA assay detects di-methylation of a
biotinylated Histone H3 (1-21) peptide at lysine 9. In this assay, a
biotinylated histone H3-derived peptide serves as the substrate, and the
modified substrate is detected using Streptavidin (SA) Alpha Donor beads and
AlphaLISA Acceptor beads conjugated to an antibody specific to the epigenetic
mark of interest.
Learn More
Technical Note
ALPHALISA HDAC1 HISTONE H3-LYSINE 27 DEACETYLASE ASSAY
AlphaLISA Assay Insights: Tracking Deacetylation Dynamics on Histone H3 Peptides
In this technical note discover how this AlphaLISA assay is designed to detect
the removal of acetyl groups from a biotin-tagged peptide derived from Histone
H3, specifically the segment from amino acids 21 to 44, which has been
acetylated at the lysine 27 position. For research use only. Not for use in
diagnostic procedures.
Learn More
Guide
ALPHALISA IMMUNOASSAY CONVERSION QUICK START GUIDE
This quick guide presents a fast proof-of concept experiment to easily convert a
immunoassay to a no-wasy AlphaLISA assay.
Learn More
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