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 * RECOMBINASE POLYMERASE AMPLIFICATION
   
   
   DISCOVER RECOMBINASE POLYMERASE AMPLIFICATION HERE AND FIND THE PERFECT
   SOLUTION FOR YOUR NEEDS
   
   
   
   


 * IT'S TIME FOR RPA
   
   
   
   
   DESCRIPTION
   
   Recombinase Polymerase Amplification (RPA) is an isothermal nucleic acid
   amplification technique that allows for the rapid and efficient amplification
   of DNA or RNA at a constant temperature, typically between 37°C and 42°C.
   Unlike traditional PCR, which requires thermal cycling, RPA uses a
   recombinase enzyme to pair primers with homologous sequences in the target
   DNA, a single-stranded DNA-binding protein (SSB) to stabilize the displaced
   DNA strands, and a strand-displacing polymerase to synthesize new DNA.
   
   
   FEATURES
   
    * Isothermal Process: Operates at a constant temperature, eliminating the
      need for a thermal cycler.
    * Speed: Can achieve detectable levels of amplification within 10-20
      minutes.
    * Versatility: Capable of amplifying both DNA and RNA (with the addition of
      a reverse transcriptase).
    * Simplicity: Requires minimal equipment, making it suitable for
      point-of-care testing (POCT) and field applications.
   
   
   APPLICATIONS
   
    * Point-of-Care Diagnostics: Ideal for rapid, on-site testing in clinical
      and field settings.
    * Pathogen Detection: Used for detecting viral, bacterial, and other
      pathogenic DNA/RNA.
    * Resource-Limited Settings: Suitable for use in areas with limited access
      to laboratory infrastructure


 * CORE ENZYMES
   
   
   
   
   T4 UVSX RECOMBINASE
   
   T4 UvsX recombinase, derived from T4 phage, is a homolog of the RecA/Rad51
   family. RecA/Rad51 recombinase family plays an important role in the repair
   of double-stranded DNA break and the restart of replication forks. T4 UvsX
   recombinase can anchor on single-stranded DNA together with other recombinase
   and activate it to scan for homologous sequences on other double-stranded DNA
   for further strand replacement. The enzyme has no nuclease activity.
   
   Learn More
   
   
   T4 UVSY PROTEIN
   
   T4 UvsY Protein, a phage T4 recombination mediator protein, plays an
   important role in T4 homologous recombination. During the recombination, T4
   UvsX recombinase must compete with the prebound gp32 single-stranded binding
   protein for DNA-binding sites. T4 UvsY protein stimulates this filament
   nucleation event, promoting the combination of T4 UvsX recombinase with
   single-stranded DNA.
   
   
   
   Learn More
   
   
   T4 GENE 32 PROTEIN
   
   T4 Gene 32 Protein is a single-stranded DNA binding protein required for
   bacteriophage T4 replication and repair. It coordinately binds and stabilizes
   the transiently formed ssDNA region and plays an important structural role in
   the process of T4 phage DNA replication. The protein has also been widely
   used to stabilize and label ssDNA regions in order to observe the structure
   of intracellular DNA with electron microscopy.
   
   Learn More
   
   
   BSU DNA POLYMERASE
   
   Bsu DNA Polymerase, derived from Bacillus subtilis, was obtained by
   truncating the first 296 AAs of the Bacillus subtilis DNA polymerase (Bsu) I
   gene. This polymerase retains its 5’→ 3’ DNA polymerase activity with its 5’→
   3’ exonuclease domain removed. Bsu DNA Polymerase naturally lacks the 3’→ 5’
   exonuclease activity, which can be used for recombinase amplification.
   
   Learn More
   
   
   SAU DNA POLYMERASE
   
   Sau DNA polymerase is a DNA polymerase with strand displacement activity,
   which can be used for recombinase isothermal amplification. The protein-DNA
   complex formed by the combination of recombinant enzyme UvsX and primers can
   find homologous sequences in double-stranded DNA. Once the primer locates the
   homologous sequence, a strand displacement reaction will occur. Sau DNA
   polymerase starts DNA synthesis, and exponentially amplifies the target
   region on the template. The replaced DNA strand will bind to SSB to prevent
   further replacement.
   
   Learn More
   
   
   BST DNA/RNA POLYMERASE
   
   Bst DNA/RNA Polymerase is a mixture of Bst polymerase and extremely
   thermostable reverse transcriptase (65°C tolerant), which is suitable for the
   isothermal amplification reaction of RNA. It can detect low-sensitivity RNA
   molecules. This enzyme is recommended in isothermal amplification experiments
   using RNA as a template. In addition, Bst DNA/RNA Polymerase can also perform
   isothermal amplification of DNA templates.
   
   
   
   Learn More
   
   
   EXONUCLEASE III
   
   Exonuclease III was derived from E.coli, with 3 '- 5' exonuclease activity,
   acts on double-stranded DNA, and gradually cuts single nucleotides from the 3
   'OH terminal direction. Each time the enzyme binds to the substrate for
   catalysis, only a few nucleotides are removed, resulting in progressive
   deletion within the DNA molecule group.
   
   Learn More
   
   
   ENDONUCLEASE IV
   
   Endonuclease IV, also known as Nfo, is derived from E.Coli, involved in DNA
   damage repair. The enzyme can recognize the AP site (apurinic/apyrimidinic
   site) on double-stranded DNA, and cleave the first phosphodiester bond at the
   5´ end of the AP site to generate 3´ hydroxyl groups and 5´ deoxyribose
   phosphate terminals.
   
   Learn More


 * DNA-FREE RPA CORE ENZYMES
   
   
   WE ALSO OFFER DNA-FREE RPA CORE ENZYMES, WHICH HAS UNDERGONE A RIGOROUS E.
   COLI DNA REMOVAL PROCESS
   
   


 * EVOLUTION OF RPA SYSTEM
   
   1
   
   
   GEN-1 RPA SYSTEM
   
   Highly stable amplification system
   
   2
   
   
   GEN-2 RPA SYSTEM
   
   Introduction of an additional Sucrose-Sucrose Phosphorylase Energy System,
   making the amplification system even more stable and reliable.
   
   3
   
   
   GEN-3 RPA SYSTEM
   
   Introduction of an additional dUTP-UDG-UGI System to effectively prevent
   aerosol contamination.
   
   4
   
   GEN-4 RPA SYSTEM
   
   Revolutionary Exclusive Multiplex Recombinase Polymerase Amplification (RPA)
   Kit Capable of Completing Direct Amplification of DNA/RNA within 10 to 20
   Minutes.


 * RPA SYSTEM PORTFOLIO
   
   
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 * MORE INFORMATION
   
   
   
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 * CONTACT
   
   
   FOR MORE INFORMATION ABOUT OUR RPA SYSTEM, PLEASE FILL OUT THE PROVIDED FORM.
   
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